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Vericheck ddPCR Replication Competent AAV Kit

Bio-RadAvailable: Worldwide

Adeno-associated virus (AAV) is commonly used as a viral vector in cell and gene therapy production, but there is a risk of it becoming a replication competent virus (RCV) during the production process, especially in the presence of a helper virus, which can raise safety concerns. While the guidelines recommend a cell culture–based method for RCAAV testing, it is cumbersome and takes 30–45 days from sample to results. Verichec…

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Adeno-associated virus (AAV) is commonly used as a viral vector in cell and gene therapy production, but there is a risk of it becoming a replication competent virus (RCV) during the production process, especially in the presence of a helper virus, which can raise safety concerns. While the guidelines recommend a cell culture–based method for RCAAV testing, it is cumbersome and takes 30–45 days from sample to results. Vericheck ddPCR RCAAV Kit is a fast, cost-effective, specific, and fully validated solution for AAV testing.

Features and benefits

  • Fast turnaround time: Results in as little as 8 hours
  • High sensitivity: LOD of 0.50 copies/µl for RCAAV
  • High specificity: 99.9%
  • Novel assay design: Kit validated to be serotype agnostic (AAV1-AAV10) and offers low CV (<10%) for reproducible results
  • Automated data analysis: Simplified workflow with positive controls included in the kit to aid automated results
  • Comprehensive ddPCR workflow: Supports and provides flexibility to run multiple samples per run

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