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Vericheck ddPCR Replication Competent Lentivirus Kit

Bio-RadAvailable: Worldwide

Lentiviruses are commonly used as viral vectors in cell and gene therapy production, delivering and steadily integrating desired genes into the genome of the host cell. However, they may also present health hazards to humans due to integration-mediated transformation and the creation of a RCL that can infect nontarget cells. While the current regulatory guidelines recommend a cell culture–based method for RCL testing, it is cu…

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Lentiviruses are commonly used as viral vectors in cell and gene therapy production, delivering and steadily integrating desired genes into the genome of the host cell. However, they may also present health hazards to humans due to integration-mediated transformation and the creation of a RCL that can infect nontarget cells. While the current regulatory guidelines recommend a cell culture–based method for RCL testing, it is cumbersome and takes 30–45 days from sample to results. Bio-Rad’s Vericheck ddPCR™ RCL Kit is fast, cost-effective, specific, and a fully validated solution for lentivirus testing.

Features and benefits

  • Fast turnaround time: Results in as little as 8 hours
  • High sensitivity: LOD of 0.35 copies/µl
  • Reproducible results: Low CV of <10% between lots, users, plates and instruments
  • Automated data analysis: Simplified workflow with positive controls included in the kit to aid automated results
  • Comprehensive ddPCR workflow: Supports and provides flexibility to run multiple samples per run

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