Vybrant DyeCycle™ Green and Orange Stains
DNA-selective, cell membrane-permeant, and nonfluorescent stains for DNA content analysis in living cells.
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Great result for cell cycle analysis!
Cell cycle by flow cytometry
A very easy and efficient way of cell cycle staining.
Review Date: 10 Feb 2020 | Thermo Fisher Scientific
Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of physical, chemical, or biological means, to monitor apoptosis, and to study tumor behavior and suppressor gene mechanisms. In a given population, cells are distributed among three major phases of cell cycle: G0/G1 phase (one st of paired chromosomes per cell), S phase (DNA synthesis with variable amount of DNA), and G2/M phase (two sets of paired chromosomes per cell, prior to cell division). DNA content can be measured using fluorescent, DNA-selective stains that exhibit emission signals proportional to DNA mass. Flow cytometric analysis of these stained populations is then used to produce a frequency histogram that reveals the various cell cycle phases. This analysis is typically performed on permeabilized or fixed cells using a cell-impermeant nucleic acid stain, but is also possible using live cells and a cell-permeant nucleic acid stain. While the choices for fixed cell staining are varied, there are only a few examples of useful cell-permeant nucleic acid stains.
The Vybrant® DyeCycle™ Green and Orange stains are DNA-selective, cell membrane-permeant, and nonfluorescent stains for DNA content analysis in living cells. The Vybrant® DyeCycle™ Green and Orange stains are fluorescent upon binding to double-stranded DNA. These stains take advantage of the commonly available 488 nm excitation source, placing cell cycle studies on live cells within reach of all flow cytometrists. Vybrant® DyeCycle™ Green stain is excited at 488 nm with emission ~520 nm. Vybrant® DyeCycle™ Orange stain is excited using both 488 nm and 532 nm laser lines with emission ~570 nm.
The staining protocol is simple and includes incubating suspended cells in the presence of Vybrant® DyeCycle™ stain and directly measuring the fluorescence without the need for any additional treatment or centrifugation steps. This live cell stain allows the simultaneous co-staining of the cell population for other parameters, and allows for the possibility of cell sorting based on DNA content.
