Wheat Germ Agglutinin (WGA) Conjugates
A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes, biotin, or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine and stains yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells.
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Wheat germ agglutinin (WGA) is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram bacteria and mammalian cells.
- Stain yeast bud scars as well as the cell membrane of gram bacteria and mammalian cells or tissues
- Suitable for Western Blot, ELISA, immunohistochemistry and other standard immunoassay applications
- A retrograde or anterograde neuronal tracer
- Withstands fixation and permeabilization
- Choice of 14 CF® Dyes from UV to near-infrared, biotin, or HRP
- Superior CF® Dyes are bright, photostable, and water-soluble
WGA is commonly used to label glycoproteins for imaging of the plasma membrane in live or fixed cells, for staining of tissue sections, or for western blotting. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae.
