Yeast Two-Hybrid Systems
More than 18,000 studies have been published citing the use of the yeast two-hybrid system to investigate protein-protein interactions, and Clontech’s Matchmaker™ Systems have been by far the most popular. Matchmaker™ Gold Systems are the highest performing incarnations of our Matchmaker product line, due to a combination of a new yeast strain (Y2HGold), stringent reporters, easy to use libraries, high-level expression vectors…
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Good but watch out for extra kits NOT included but needed!
Assessment of protein-protein interactions
Relatively straightforward protocol, yeast can be finicky but that's not new- one frustrating point is that there are various kits not contained here that you need- PCR, Insert verification etc. These should REALLY be in there. If I'm buying a >$1000 kit, I don't want to spend another $500 on stuff I need to run this experiment, but wasn't included.
Review Date: 23 Sept 2022 | Takara Bio
More than 18,000 studies have been published citing the use of the yeast two-hybrid system to investigate protein-protein interactions, and Clontech’s Matchmaker™ Systems have been by far the most popular.
Matchmaker™ Gold Systems are the highest performing incarnations of our Matchmaker product line, due to a combination of a new yeast strain (Y2HGold), stringent reporters, easy to use libraries, high-level expression vectors, and SMART™-based library construction kits.
In a Matchmaker GAL4-based two-hybrid assay, a bait protein is expressed as a fusion to the GAL4 DNA-binding domain (DNA-BD), while libraries of prey proteins are expressed as fusions to the GAL4 activation domain (AD; Fields & Song, 1989; Chien et al., 1991). When bait and library (prey) fusion proteins interact, the DNA-BD and AD are brought into proximity to activate transcription of four independent reporter genes (AUR1-C, ADE2, HIS3, and MEL1).
This technology can be used to:
•Identify novel protein interactions
•Confirm suspected interactions
•Define interacting domains
