Zero Blunt™ PCR Cloning Kit
The Zero Blunt PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proof-reading, thermostable DNA polymerases.
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
My life would have been much harder without this kit.
Cloning
I have been using the ZeroBlunt cloning kit for over 7 years and I can confidently say that it is one of the most efficient and convenient intermediate vectors for cloning. The advantages include the compatibility with the high fidelity phusion polymerase (which is my first choice when it comes to PCR due to the high efficiency and low incidence of mistakes), ease of use and high efficiency. One more bonus is that, unlike the majority of the vectors (including the PCR2.1 cloning kit), ZeroBlunt vector has Kanamycin resistance, which makes cross-contamination during subcloning to the final expression vector nearly impossible.bOverall, I highly recommend this kit for everyone who needs to clone any DNA fragment.
Review Date: 11 Jun 2021 | Thermo Fisher Scientific
Description
The Zero Blunt PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proof-reading, thermostable DNA polymerases. The Zero Blunt PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step.
Features of the Zero Blunt Cloning Kit with pCR™-Blunt vector:
• Fast & convenient—5-minute, room-temperature ligation
• Efficient—ccdB gene for positive selection results in >80% clones with correct insert
• Flexible—choice of kanamycin or Zeocin™ resistance for flexible antibiotic selection
The pCR™-Blunt vector provides:
• EcoR I sites flanking the PCR product insertion site for excision of inserts
• T7 promoter/primer site for in vitro RNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing or PCR screening
