ZORBAX 300StableBond
Agilent ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phas…
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Great separation and customer service.
Large molecule characterization
Great separation for large molecule virus therapeutics. Agilent is very helpful with method development suggestions and returns were easy.
Review Date: 26 Mar 2019 | Agilent Technologies
Exceptional resolution!
Rapidly analyze oxidative species via UPLC
The Zorbax RRHD diphenyl column works as advertised! We've successfully resolved varying oxidative species using this column, at 6 minutes per run. This will be a workhorse moving forward
Review Date: 23 Jun 2016 | Agilent Technologies
Analytical R&D
High efficiency and better resolution.
Review Date: 17 Jun 2014 | Agilent Technologies
Bioanalysis
One excellent point from Agilent is the booklet inside the box and with all information regarding the column is clearly described. In terms of capacity of separation, this column is really excellent but the biggest problem of this column is its’ lifetime. Only after less than 1000 of injections of clean samples (plasma or buffers extracted by SPE for example), the peak shape deteriorates very quickly i.e a lot of tailing. This happens several times even when using an in-line frit before the column. Even after a thorough cleaning of the column, you will not be able to get back a good peak shape. By comparison with columns from the competitors, similar columns from Waters (Acquity) and from Phenomenex (Kinetex) are much better alternatives, especially if you just check the price. For the same amount of money, you will be able to inject 3-5 times more on Waters and Phenomenex columns.
Review Date: 29 Jan 2014 | Agilent Technologies
Agilent ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers.
These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations.
Features:
- Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins
- Exceptional stability and long lifetime at low pH with TFA containing mobile phases
- Excellent reproducibility
- Available in capillary and nano column dimensions for proteomics and other LC/MS applications
