ZORBAX StableBond
ZORBAX 80Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1–6 range for a wide variety of phases (SB-Aq, SB-C3, SB-CN, SB-Phenyl, SB-C8, and SB-C18). Excellent peak shape is expected for many basic compounds because…
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Method Development
We found this column to be extremely robust and good for very low pH range than some of the other columns we tried. As we are developing a method for use with acidic mobile phases, this column's stability and robustness operating at low pH gives us good peak shapes for our sample(s) under evaluation.
Review Date: 16 May 2014 | Agilent Technologies
HPLC Method development
We had a very positive experience with this column! It gives slim peaks, good selectivity and is very stable. Ideal for HPLC and UPLC.
Review Date: 2 Apr 2013 | Agilent Technologies
Tobacco specific nitrosamines analysis by LC-MS/MS
Good peak shape and long life time for low pH mobile phase LC applications.
Review Date: 28 Mar 2013 | Agilent Technologies
ZORBAX 80Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1–6 range for a wide variety of phases (SB-Aq, SB-C3, SB-CN, SB-Phenyl, SB-C8, and SB-C18). Excellent peak shape is expected for many basic compounds because of the ultrapure Rx-SIL support.
ZORBAX SB-Aq is an HPLC alkyl reversed-phase bonded phase designed to retain hydrophilic and other compounds when using highly aqueous mobile phases, including 100% water. The SB-Aq surface chemistry is designed to allow the alkyl phase to remain fully accessible in highly aqueous mobile phases, preventing "phase collapse.” Because of this, separations on SB-Aq are reproducible from run to run over long column lifetimes. By contrast, many conventional C18 columns—and even some columns with claimed aqueous compatibility—may exhibit phase collapse, and thus show reduced retention or selectivity changes over time, leading to irreproducible results.
StableBond columns are available in 1.8, 3.5, 5 and 7 μm particle sizes for high speed, high resolution, analytical and prep scale separations.
Features:
- Longest column lifetime and best reproducibility for low pH separations—down to pH 1
- Patented stable column chemistry allows use at high temperature and low pH without degradation
- Six different bonded phases provide broad selectivity – SB-C18, SB-C8, SB-CN, SB-Phenyl, SB-C3, and SB-Aq
- Different selectivity compared to conventional end-capped chemistries
- High purity (Type B) silica for good peak shape
- SB-Aq offers reproducible retention of polar analytes in aqueous mobile phases
