ZymoBIOMICS™ Microbial Community DNA Standard
Assess bias and errors in NGS-based microbial composition profiling workflows
ZymoBIOMICS Community DNA Standard
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Useful in a wide variety of experimental contexts
Assess variability between sequencing runs.
We've used this standard on several projects to help determine how differences in library prep protocols effect the overall microbial community composition. It is very helpful to have a consistent, known quantity of DNA in each reaction plate, and running these standards have revealed which protocol steps have the largest impact on our results. Finally, I think it has good taxonomic breadth to be useful in a wide range of applications. In my opinion, the purchase price is worth the time and effort it would take to produce a comparable reagent in our lab.
Review Date: 9 Feb 2023 | Zymo Research
Consistent and reliable results to validate 16S ribotyping data
Microbiome research
I am using this product as a control for various 16S ribotyping projects. This has been incredibly effective at normalizing experimental data. The community diversity results I am seeing are precisely what Zymo states it should be.
Review Date: 25 Jan 2022 | Zymo Research
Easy to use
Used to validate a new in-house methodology to assess microbial composition in human stool samples
Easy to use. Good as a standar to validate your techniques.
Review Date: 25 Jan 2022 | Zymo Research
Very useful.
Plant microbial community analysis
Very easy to use, easy to find sequences online to integrate into analytical workflow. Very useful tool to evaluate success of 16S sequencing experiment! I appreciate that zymo products are carefully designed for reproducible microbiome studies.
Review Date: 16 Dec 2021 | Zymo Research
Improved confidence in microbiome data and the downstream analysis.
Microbiomics
The product was easy to use, easy to calculate how to spike in to samples, and added a significant level of confidence to the microbiome data we have been analyzing.
Review Date: 17 Nov 2021 | Zymo Research
Useful for 16S amplicon sequencing work.
Used as a standard for 16S amplicon sequencing work
I found this product really useful as a positive control for 16S rRNA amplicon sequencing work.
Review Date: 28 Jul 2021 | Zymo Research
Great product, consistent results!
Sequencing control
Great product, consistently works well and we include it in all our V4 16S amplicon library preps for Illumina sequencing during PCR. We typically include at least 1 Zymo mock community per plate of samples.
Review Date: 16 Mar 2021 | Zymo Research
Great for bacteria, not so much for fungi, but reliable.
Control for microbiome studies
This product contains only two fungal strains, which is a big bummer for me, but overall is a reliable product that we count on daily in our lab.
Review Date: 4 Dec 2020 | Zymo Research
Very recommendable to assess microbiome workflows from 'bench-to-bedside'.
Quality control during microbiome analysis
I use the ZymoBIOMICS Microbial DNA Standard as a separate sample during my 16S rRNA gene library preparation and sequencing experiments. The DNA standard contains a known amount of eight bacterial and two fungal species genomes to assess the performance of the microbiome pipelines from the very beginning until the data analysis step. It is very straightforward to use, and I have obtained accurate and reproducible results so far. I have also used this for optimizing PCR experiments, obtaining good results as well. Just a 'heads-up' for new users: when performing taxonomic assignation of the amplicon sequence variants obtained from the DNA Standard sample, double-check your results with more than a single 16S rRNA gene database in case you are missing any of the microbial species contained in the DNA Standard. I was using the Human Oral Microbiome Database (HOMD) for assigning taxonomy and I was missing Salmonella enterica on the DNA Standard sample. I double-checked the non-assigned (NA) amplicon sequence variants using standard nucleotide blast against the nucleotide collection database from the National Center for Biotechnology Information (NCBI) and, indeed, they were assigned to S. enterica.
Review Date: 11 Dec 2019 | Zymo Research
Great mock for inferring community composition with NGS.
Next Generation Sequencing
The community mock is always retrieved in the expected relative abundances. It provides a lot of certainty for the composition of my samples and the quality of sequencing. Examining the accuracy of the mock provides information on issues such as over- or under-representation of certain sequences and guides the subsequent filtering steps I need to take in order to accurately analyze my own samples.
Review Date: 6 Jun 2019 | Zymo Research
ZymoBIOMICS™ Microbial Community DNA Standard is a mixture of genomic DNA extracted from pure cultures of eight bacterial and two fungal strains. Genomic DNA from each pure culture was extracted and quantified before mixing. The GC content of the containing genomes covers a range from 15% to 85%. The microbial standard is accurately characterized and contains negligible impurities ( This standard is also ideal to help users construct and optimize workflows, e.g. controlling PCR chimera rate and noise in the library preparation of 16S rRNA gene targeted sequencing, and assessing GC bias in sequencing coverage of shotgun metagenomic sequencing.
