COMET™

The COMET™ is a walk-away automation system to scale-up spatial biology assays with unmatched throughput. It can perform proteomics, transcriptomics and same-section multiomics applications. For protein detection, COMET™ works with standard, off-the-shelf, label-free primary antibodies and performs sequential immunofluorescence (seqIF™) assays that consist of sequential cycles of staining, imaging, and elution. In each cycle, protocol conditions can be finetuned to stain and image up to 4 antibodies. Runs of 40 markers can be performed fully automated in just a few hours on the same tissue sample and without user intervention.

For multiomics application, detection of RNA and protein is performed simultaneously on the same tissue section at single-cell level. The system integrates the entire staining or target probe hybridization, imaging and image pre-processing workflow without the need for any user intervention. The workflow includes an optimized target retrieval step and a protease-free pretreatment to maximize RNA and protein target detection as well as epitope unmasking while preserving tissue morphology. The use of RNAscope™ HiPlex and seqIF™ allows fully flexible RNA and protein target selection to build your panel.