The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites. New cDNA synt…
The LumiMax superoxide anion detection kit qualitatively detects the presence of superoxide anions and superoxide dismutase in cell cultures with a higher sensitivity than the standard luminol-based assay. More sensitive than traditional assays (use as few as 10e4 cells) Enhancer is non-cytotoxic and non-denaturing Up to 40-fold signal enhancement for cultured cells Proprietary enhancer which increases signal at lea…
The Mitochondrial membrane potential detection kit uses a unique fluorescent cationic dye, JC-1, to signal the loss of mitochondrial membrane potential. In healthy non-apoptotic cells, the dye stains the mitochondria bright red. Detect the loss in mitochondrial membrane potential - Early indicator of cellular apoptosis Convenient kit format Use with flow cytometer, fluorescence plate reader, fluorescence microscope
pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. CMV promoter for high-level protein expression in mammalian cells Large multiple cloning site contains 15 unique sites Clone PCR products generated with any PCR e…
The Agilent Oligo aCGH Prehybridization Buffer is used to wet and condition arrays for hybridization on a Tecan HS 400 Pro or HS 4800 Pro with compatible hybridization chambers. It provides for better distribution of the sample over the active area, and helps prevent the occurrence of gradients in the signals.
Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.
Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.
The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD). Detects protein-prote…
The InterPlay TAP Purification Buffer Kit is designed to work with the InterPlay Mammalian TAP System and InterPlay Adenoviral TAP System to recover interacting proteins from mammalian cells. The InterPlay TAP Purification Buffer Kit includes the buffers required to perform tandem affinity purification.
Agilent's GeneSpring provides powerful, accessible statistical tools for intuitive data analysis and visualization.
SureCall is a research desktop application combining both novel and widely accepted opensource algorithms for end-to-end NGS data analysis from alignment to categorization and annotation of mutations, supporting Agilent Target Enrichment applications.
The MBS Mammalian Transfection Kit increases transfection efficiencies by up to 100-fold. Cells are incubated with CaPO4/DNA coprecipitate for only 3 hours compared to 6 to 12 hours with traditional CaPO4 methods.
Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.
A collection of classic strains and competent cells.
The ABLE C strain and the ABLE K strain help solve issues associated with toxic clones. These strains reduce the copy number of common cloning vectors by ~4- and 10-fold, enhancing the probability that a toxic clone will be propagated.
The ABLE C strain and the ABLE K strain help solve issues associated with toxic clones. These strains reduce the copy number of common cloning vectors by ~4- and 10-fold, enhancing the probability that a toxic clone will be propagated.
The In Situ beta-Galactosidase Staining Kit provides an easy and rapid method to determine transfection efficiency by indicating beta-Galactosidase activity in individual intact cells. In situ staining of cells directly in dishes without removing or manipulating cells Rapid detection of Ÿ-gal activity in transfected cells Results in 24 hours Maximum signal with low background Deep blue staining reaction Kit in…
Perform high sensitivity protein electrophoresis with the 2100 Bioanalyzer System. The High Sensitivity Protein 250 assay provides a broad dynamic range with sensitivity down to Silver Stain comparable levels. Efficiency is enhanced by eliminating handling of SDS-PAGE slab gels, staining or, imaging steps.
Perform high sensitivity protein electrophoresis with the 2100 Bioanalyzer System. The High Sensitivity Protein 250 assay provides a broad dynamic range with sensitivity down to Silver Stain comparable levels. Efficiency is enhanced by eliminating handling of SDS-PAGE slab gels, staining or, imaging steps.
The Supercoiled DNA Ladder contains fragments from 2 kb to 10 kb at 1-kb increments and an additional band at 12 kb. Each supercoiled band is at a known concentration, with the 7-kb band at a higher concentration than the others. The resulting brighter band in the middle of the ladder acts as a useful reference point. Accurate size determination facilitated by evenly spaced bands Fragments are exact multiples of 1000 bp…
Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.
The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.
The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.
The Just cDNA Double-Stranded cDNA Synthesis Kit generates high-quality, blunt-ended cDNA, perfect for use in non-directional cDNA libraries. Makes blunt double-stranded cDNA Can be used for in vitro transcription Perfect for nondirectional cDNA libraries
MiracleHyb hybridization solution was developed for use in Northern analysis when sensitive detection of low-abundance messages is a priority. A probe preparation buffer is included to make probe handling easier and minimize background problems. Easily detect and analyze rare gene transcripts Delivers a rapid hybridization signal and a high signal-to-noise ratio over time Room-temperature storage; easy to incorporate i…
