Drug discovery > Target Discovery Products & Reviews

This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.

The pCMV-Script PCR cloning kit is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. In the ligation reaction, PCR products are incubated with the predigested pCMV-Script mammalian expression vector, the restriction enzyme Srf I, and T4 DNA ligase.  Cl…

BL21-Gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated. 

The XL1-Blue strains are an all-prupose line of competent cells that are ideal for routine cloning needs. 

Most E. coli hosts contain both DNA adenine methylation (dam) and DNA cytosine methylation (dcm) genes. These genes code for proteins that methylate specific sequences when DNA is propagated, making subsequent digestion with methylation-sensitive restriction enzymes impossible. Agilent offers the SCS110 and JM110 strains, which lack both dam and dcm activity. 

This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.

High efficiency competent cells in convenient packaging designed to satisfy higher throughput requirements. For ultimate convenience we offer custom configurations, formulations, and packaging options.

High efficiency competent cells in convenient packaging designed to satisfy higher throughput requirements. For ultimate convenience we offer custom configurations, formulations, and packaging options.

Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems. The SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryoti…

Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems. The SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryoti…