Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect Cis-Reporting Systems. When PathDetect cis-reporting and expression plasmids encoding your gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element
Determine the direct or indirect involvement of new gene products, growth factors, and drug candidates in specific pathways with our PathDetect Trans-Reporting Systems. Easily investigate whether your protein or compound influences the activation of any of the six transcription factors offered. These systems are available as plasmid vectors with a luciferase reporter for co-transfection with your gene of interest into the c…
The GeneJammer Transfection Reagent is a proprietary formulation of novel polyamines ideal for transient or stable transfections in a wide variety of cell types. This transfection reagent is effective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicity.
Hygromycin B is an antibiotic that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
The LipoTAXI mammalian transfection reagent contains a unique, low-toxicity lipid formulation that has been tested with over 30 cell lines and has been shown to achieve high efficiency transfections with a wide variety of cells. Compared to CaPO4/DEAE-mediated transfection, it provides a 10-fold increase in transient transfection efficiency in many cell lines. This transfection reagent may be used with both adherent cells and…
The MBS Mammalian Transfection kit can increase your transfection efficiencies up to 100-fold. Cells are incubated with CaPO 4 /DNA coprecipitate for only 3 hours, versus 6 to 12 hours with traditional CaPO 4 methods. Additionally, cells can be assayed 12 to 24 hours post-transfection instead of the usual 48 to 72 hours.
The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.
The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.
The AdEasy Virus Purification Kit allows the purification and concentration of Adenovirus (from Ad5 strains) with Sartobind ® syringe filters containing an ion exchange membrane adsorber that selectively binds adenoviral particles. Once bound, viral particles can be further purified by washing away nonspecifically-bound proteins before elution.
The ViraPort retroviral gene expression system is a comprehensive line of gene delivery products providing transduction efficiencies approaching 100% in a wide range of mitotic cells.
The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae. Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter.
These Gilson engineered caps produce a positive pressure seal with the needle in the Gilson ASPEC, ASPEC XL and ASPEC XL4 solid phase extraction systems.
Conversion of cartridge-based methods to an automation friendly 96-well format has never been easier or faster. The same trusted silica-based sorbents in Bond Elut cartridge products are now available in the streamlined Bond Elut 96-well plates. Bond Elut 96 components are specially formulated to offer superior cleanliness, flow reproducibility, and reliability.
Bond Elut 96 Square-well Plates are specially designed to offer superior cleanliness, flow reproducibility, and reliability, ensuring trouble-free, high throughput operation. Conversion of cartridge-based methods to an automation-friendly 96-well plate format has never been easier or faster.
Bond Elut AccuCAT cartridges are mixed bed SPE cartridges consisting of a strong cation exchange (SCX) and a strong anion exchange (SAX) sorbent packed into one bed. AccuCAT is effective for the extraction of acidic, basic, and neutral analytes from urine and other biological samples. AccuCAT is particularly effective for catecholamine extraction from biofluids.
Bond Elut adapters fit on top of any Bond Elut cartridge and contain a female Luer fitting that accommodates the tip of another cartridge.
Alumina, like silica, is an extremely polar sorbent. The alumina surface tends to be slightly more stable under high pH conditions than unfunctionalized silica. The small particle size of the Bond Elut Alumina range ensures high extraction efficiency even when small bed masses are used.
Due to the methyl group and subsequent low carbon load, Bond Elut C1 is the least retentive of all alkyl group bonded phases for nonpolar compounds. However, due to the extensive endcapping of this sorbent to mask polar silanol activity, retention, and elution of polar and multifunctional analytes can still be achieved.
Bond Elut EWP is based upon standard particles size silica but with 500 pores to allow more efficient extraction of large molecules (>15,000 MW), which are typically excluded from standard porosity silica phases.
Bond Elut C18 OH is a nonendcapped version of the octadecyl bonded phases that enables the silanols on the silica surface to be more active. This low-load C18 has well-controlled silanol activity that permits the fractionation of metabolites and enhances retention of basic compounds compared to an endcapped C18. The pore size makes it a good choice for peptide extraction.
Bond Elut C2 is a fairly nonpolar sorbent because of the short chain length of the functional group. C2 is often used during the process of method development if analytes are retained too strongly on a C8 or C18 phase. The polarity of C2 is slightly lower than a cyano phase for polar interactions.
Bond Elut C8 is very similar in property to C18, but is not as retentive for nonpolar compounds due to its shorter hydrocarbon chain and therefore reduced carbon loading. C8 is an excellent replacement for C18 when analytes are too strongly retained for effective elution.
Bond Elut Carbon cartridges are packed with ultrapure graphitized carbon particles that have been optimized for the absorption of pigments in food, fruits and vegetables, and small organic residues in wastewater. The powerful retention mechanisms of these products are appropriate for a broad range of analytes. In addition, careful manufacturing techniques result in lower carbon fines on the wall of the device.
CBA is a midpolarity sorbent and weak cation exchanger (pKa 4.8). It can be used with a wider range of counterions than lower pKa sorbents like SCX, and will demonstrate easier elution of quaternary amine functionalized analytes.
Bond Elut Cellulose columns utilize a pure microgranular cellulose powder that is packed between two, 20 µm polyethylene frits. The cellulose phase is very stable over a wide pH range and contains an extremely low metal content (Fe and CU content less than 5 ppm).
