Agilent Technologies Products & Reviews

The Agilent MassHunter METLIN Metabolomics Database and Library enable streamlined metabolite identification to facilitate understanding of global metabolic changes that can occur in a biological system. Available as a comprehensive database of metabolites (PCD) that optionally includes MS/MS spectra (PCDL).

The 1260 Infinity II Preparative Fraction Collector is optimized for laboratories performing semipreparative-scale purification of milligram to low gram quantities with typical flow rates between 4 and 100 mL/min. The module features the full set of collection modes and is compatible with a wide variety of trigger sources. SBS plates and four different tube sizes with various lengths are supported.

The 1260 Infinity III Manual Injector is an HPLC valve for manual injection of samples at pressures up to 600 bar. It is supplied with a baseplate, which is dimensioned to match the footprint of all 1260 Infinity modules. The 1260 Infinity III Manual Injector is a compact, affordable solution for sample injection.

The diode array design of the 1260 Infinity III Multiple Wavelength Detector offers very low detector noise (less than ±7 μAU) for precise quantification of trace levels, regardless of the number of signals recorded. Over a wavelength range from 190 to 950 nm, simultaneous detection of up to eight compound-specific wavelengths for optimum selectivity is provided.

The 1260 Infinity II Preparative Binary Pump is an affordable high-pressure gradient pump for LC purification. The pump offers a dynamic flow rate range from 1 to 50 mL/min at up to 420 bar, allowing workflows on columns from 9.4 to 30 mm inner diameter.

The 1260 Infinity III Variable Wavelength Detector enables highest productivity with fast analysis at up to 120 Hz data rates. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.

The 2100 Expert Software offers powerful data evaluation tools for all available assays, allowing you to get the most out of your digital data.

The Agilent Protein 80 and Protein 230 kits provide a reliable way to assess protein concentration, identity, and purity. It eliminates handling of SDS-PAGE slab gels, staining or imaging steps.

The Agilent DNA Fish ID solution uses advanced software to achieve more accurate results – quickly and easily. Key to the process is the Agilent 2100 Bioanalyzer and RFLP Decoder Software performs RFLP pattern matching, which eliminates the need for manual calling

The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin.

BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.

The pET E. coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.

The VariFlex protein expression system offers vectors with unique tags that enhance the utility of E. coli as an expression host. Included in our tag offering are three different SET tags which increase protein solubility and the SBP purification tag for high-yield, pure proteins.

Allows in vivo excision of cloned fragments in the phagemid vector Screening with both antibodies and nucleic acid probes Each premade library comes with necessary strains and helper phage  

This collection of competent cells provides customers with classic  E. coli  strains that have been engineered to become competent for use in cloning experiments.

Identify fish species quickly, easily, and accurately. Whether your samples are fresh, frozen, dried, salted, or minced, Agilent’s DNA Fish Species Identification solution provides reliable, robust answers within hours. And, the method is far more accurate than looking at a fish and guessing.

The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector.  In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed.  In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of i…

The single-tube format of Gigapack III packaging extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. The ability of Gigapack III packaging extracts to preserve methylated DNA makes it ideal for use in epigenetic studies where DNA methylation may be associated with heritable changes in gene expression or cellular phenotype.

The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor recognition element that modulates expression of your gene of interest.  

The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression.

Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.

Method completed in less than one day versus 2 days with restriction enzymes

The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).

StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible. The resin is a non-toxic, non-combustible slurry of hydroxylated silica particles that is used to clean up DNA or concentrate dilute protein mixtures.

The StrataClone Mammalian Expression Vectors were designed for high-level expression of epitope-tagged proteins in mammalian cells. Use of StrataClone technology for the cloning steps allows rapid cloning of blunt-ended PCR products, produced using high-fidelity PCR enzymes, into the epitope tagging/expression vectors.