The 1 kb DNA Ladder contains 13 discrete DNA fragments ranging in size from 300 bp to 10,000 bp. This marker is ideal for the size determination of digested DNA. Two Ready-to-Use DNA Size Markers for Gel Electrophoresis. The 100 bp Ladder and 1 Kb Ladder represent the two most popular markers used for DNA fragment size determination. These markers are formulated to run accurately and to provide crips band patterns. The two m…
The 100 bp DNA Ladder contains 12 discrete DNA fragments ranging in size from 100 bp to 3,000 bp. This marker is ideal for the size determination of PCR products. Two Ready-to-Use DNA Size Markers for Gel Electrophoresis. The 100 bp Ladder and 1 Kb Ladder represent the two most popular markers used for DNA fragment size determination. These markers are formulated to run accurately and to provide crisp band patterns. The two…
AxyPrep Bacterial Genomic DNA Miniprep Kit - Suitable for the rapid isolation of up to 20 mg of genomic DNA from 1.0×109 bacterial cells. Following lysis, separation of the genomic DNA from proteins, polysaccharides and lipids with the AxyPrep Plasmid MiniPrep Kit is achieved by a unique phase-partition step. The genomic DNA in the lower phase is selectively bound to a special Axygen AxyPrep column for further purification a…
Format of the AxyPrep™ Multisource Total RNA Maxiprep Kit: • Maxi column AxyPrep™ Multisource Total RNA Maxiprep Kit Starting Materials: • Animal tissues • Plant tissue • Cultured cells • Bacteria • Yeast • Filamentous fungi Features of the AxyPrep™ Multisource Total RNA Maxiprep Kit: • Rapid spin and vacuum protocols • No alcohol precipitation • No column clogging • Dramatic reduction in DNA carryover • Higher yields, higher…
This kit is based upon a modified SDS-alkaline lysis of bacterial cells in combination with lysate filtration and selective binding of the plasmid DNA to special AxyPrep purification columns. The yield of plasmid for kit is up to 500 mg respectively. This kit can also be used to isolate large constructs, such as BAC, PAC and P1. The protocols provide a simple, rapid and efficient method for the isolation of highly purified p…
This kit provides a rapid and economical method for the purification of plasmid DNA in a convenient mini column format. Each AxyPrep column has a binding capacity of at least 20 mg of plasmid DNA. AxyPrep columns are designed for use in either a spin or vacuum format. Each column can process up to 4 ml of bacterial culture grown in LB or up to 2 ml of culture grown in rich broth. The entire procedure can be completed within…
AxyPrep™ Multisource Genomic DNA Miniprep Kit is designed to purify genomic DNA from animal and plant tissues, cultured cells and yeast. Proteins, pigments, carbohydrates and lipids are efficiently segregated from the genomic DNA by a unique two-phase partition of the AxyPrep™ Multisource Genomic DNA Miniprep Kit. The genomic DNA is bound to an AxyPrep™ Genomic DNA column, where residual impurities and salt are removed. Geno…
Axygen DNA Markers for Gel Electrophoresis - Two Ready-to-Use DNA Size Markers for Gel Electrophoresis. The 100 bp Ladder and 1 Kb Ladder represent the two most popular markers used for DNA fragment size determination. These DNA Markers for Gel Electrophoresis are formulated to run accurately and to provide crips band patterns. The two Axygen DNA Markers for Gel Electrophoresis require no additional preparation and contain b…
Axygen X-Gal IPTG spray is a convenient ready-to-use solution of optimal quantities of IPTG, X-Gal and stabilizers presented in a spray bottle for easy, accurate and consistent screening of Blue/White colonies. Advantages of Axygen X-Gal-IPTG Spray include:• Easy, accurate and convenient. • Sprays deliver optimal concentration and volume consistently for consistent and accurate results. • No need to weigh X-Gal and IPTG and…
Smart Ampicillin™ from Axygen Biosciences is a specially formulated Ampicillin antibiotic that is optimized for expression studies. Smart Ampicillin is superior to common antibiotics like Ampicillin, Carbenicillin and Methicillin. These regular antibiotics are degraded by enzymes secreted by bacteria over time and the result is the undesirable appearance of satellite or non-recombinant colonies. Smart Ampicillin is specially…
Agarose LE from Axygen Bioscience is a high quality molecular biology grade Agarose suitable for analytical and preparative electrophoresis of nucleic acids. Nucleic acid separation with Agarose LE is between 0.2–15 kbp depending on the concentration of Agarose LE.
Agarose MS is a molecular screening agarose for improved resolution of DNA fragments with 500 bp or less, especially primer–sized fragments. At a 3 concentration, Agarose MS gives a resolution of DNA fragments similar to gels made with polyacrylamide at concentrations of 8 .
Agarose LM, low melting gels allows for the recovery of undamaged nucleic acids below their denaturation temperature. The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where In-Gel manipulations can be performed without prior extraction of the DNA from the gel slice. Low Melting (LM) Agarose is derivatized by organic synthesis which generates methoxylate groups from the bas…
The AxyPrep™-96 Plasmid Purification Kit represents a true high-throughput system for the purification of plasmid or cosmid DNA in a 96-well format. The AxyPrep™-96 Plasmid Purification Kit is designed to produce up to 20 mg of highly purified plasmid or cosmid DNA (per well) from up to 1.3 ml of bacterial culture in 96-well format. With the AxyPrep™-96 Plasmid Purification Kit two plates rapidly and efficiently process the…
AxyPrep™ Easy-96 Plasmid Purification Kit employs a modified alkaline lysis method in combination with an optimized 96-well lysate filtration plate and an alcohol precipitation step to purify plasmid and cosmid DNA from bacterial cultures. The AxyPrep™ Easy-96 Plasmid Purification Kit is designed to process 1.3 ml aliquots of bacterial culture, grown in a 96-well format. Plasmid or cosmid DNA prepared by the Axyprep Easy-96…
This kit is based upon a modified SDS-alkaline lysis of bacterial cells in combination with lysate filtration and selective binding of the plasmid DNA to special AxyPrep purification columns. The yield of plasmid for this kit is up to 100 mg. This kits can also be used to isolate large constructs, such as BAC, PAC and P1. The protocols provide a simple, rapid and efficient method for the isolation of highly purified plasmid…
AxyPrep™ DNA Gel Extraction Kit employs optimized reagents in combination with a convenient spin column to purify DNA fragments from either TAE or TBE agarose gels (regular and low-melt). Each AxyPrep column in the AxyPrep DNA Gel Extraction Kit will bind up to 8 µg of DNA. DNA fragments in a size range of 75 bp up to 10 kb can be efficiently recovered with the AxyPrep DNA Gel Extraction Kit. Depending upon the length of the…
AxyPrep Blood Genomic DNA Miniprep Kit - Suitable for purifying up to 12 mg of genomic DNA from 250 ml of anti-coagulated whole blood. Blood genomic DNA is isolated directly from the white blood cell (WBC) component of whole blood, without the need to remove the red blood cells (RBCs) in advance. The isolation of genomic DNA from blood using the AxyPrep Miniprep Kit is based on the efficient release of genomic DNA from by a…
The AxyPrep™ Blood Genomic DNA Midiprep kits are designed to produce up to 100 mg and 500 mg of high molecular weight genomic DNA respectively from anticoagulated human or animal blood. Purification with the AxyPrep Blood Genomic DNA Midiprep Kit is based upon a unique phase-partitioning technology in combination with the selective binding of the genomic DNA to a special AxyPrep column. The purified genomic DNA is greater th…
The AxyPrep™ Blood Genomic DNA Maxiprep kits are designed to produce up to 100 mg and 500 mg of high molecular weight genomic DNA respectively from anticoagulated human or animal blood. DNA Purification with the AxyPrep Blod Genomic DNA Maxi Prep Kit is based upon a unique phase-partitioning technology in combination with the selective binding of the genomic DNA to a special AxyPrep column. The purified genomic DNA is greate…
AxyPrep™ Body Fluid Viral DNA/RNA Miniprep Kit provides a rapid and highly reproducible way to purify viral nucleic acid from 250 ml of body fluid, including plasma, serum, ascites, cerebrospinal fluid, urine, cell culture supernatant, etc. With the AxyPrep™ Miniprep Kit, during lysis, proteins and PCR inhibitors are removed by denaturation and precipitation. Viral nucleic acids remain soluble in the supernatant and are puri…
The AxyPrep Multisource Total RNA Miniprep Kit from Axygen allows the purification of total cellular RNA from a diverse array of starting materials. The AxyPrep Multisource Total RNA Miniprep Kit represents a new approach to RNA purification, and is designed to eliminate the problems of column clogging and incomplete purification associated with other products. The optimized protocol of the AxyPrep Multisource Total RNA Mini…
The AxyPrep Blood Total RNA Miniprep Kit is designed to process up to 400 µl of anticoagulated whole blood per prep. RBCs are removed using a special lysis buffer. Following WBC lysis, genomic DNA and protein are removed via precipitation, which significantly improves the yield and purity of the total RNA sample. The carryover of genomic DNA is greatly reduced and the opportunity for column clogging is eliminated, ensuring o…
AxyPrep™ Multisource Total RNA Midiprep Kit from Axygen Scientific Format of the AxyPrep™ Multisource Total RNA Midiprep Kit from Axygen: • Midi column AxyPrep™ Multisource Total RNA Midiprep Kit Starting Materials: • Animal tissues • Plant tissue • Cultured cells • Bacteria • Yeast • Filamentous fungi Features of the Axygen AxyPrep™ Multisource Total RNA Midiprep Kit: • Rapid spin and vacuum protocols • No alcohol precipitat…
