Bioneer’s AccuPrep® Genomic DNA Extraction Kit allows the extraction of total genomic DNA from whole blood, lymphocytes, mammalian tissue and cultured cells. Average yield of 6 μg of total genomic DNA from 200 μl of whole human blood and up to 20 μg from 5 x 106 lymphocytes, 25 - 50 mg mammalian tissue, or 104 -108 cultured cells.AccuPrep Genomic DNA Extraction Kit employs glass fibers, fixed in the AccuPrep column, that speci…
Blood RNA PrepMate™ is designed for rapid isolation of high-purity total RNA from Blood or cultured cells based on the guanidinium salt-lysis method. The extracted total RNA from PrepMate can be used for Northern analysis, dot blot hybridization, poly-A (+) selection, RNase protection assays, and molecular cloning, or RT-PCR. PrepMate is an economical RNA kit that provides excellent yields of pure total RNA.
Bioneer’s HotStart DNA polymerase uses an exclusive enzyme-mediated Hot Start PCR method that, unlike most other HotStart PCR chemistries, completely releases all polymerase activity during the first denaturation step. Top DNA polymerase is completely inhibited by pyrophosphate at temperatures below 70°C. However, at temperatures above 70°C, a thermostable pyrophosphatase initiates pyrophosphate hydrolysis and activates the DN…
Bioneer introduces AccuPrep® Nano-Plus Plasmid DNA Extraction Kits - the first in a series of unique and revolutionary nucleic acid purification kits that maximize DNA purity, improve yield and significantly reduce prep time—while minimizing costs. AccuPrep Nano-Plus kits are available in Miniprep, Midiprep and Maxiprep formats. A centrifuge can be used to purify plasmid DNA with our AccuPrep Nano-Plus kits, and, thanks to our…
Bioneer’s core competency in delivering the most rigorously manufactured nucleic acids, includes providing the industry’s highest quality Custom RNA oligos. To cover all of your bases, we manufacture RNA oligos with our patented Bio-RP purification which approaches near HPLC quality. For your most stringent applications, we offer a wide array of modification options and services including bar-code labeling and annealing chimer…
ExiPrep™ 16 Plus is a laboratory automation system able to extract DNA or RNA from various biological samples including tissue, cell culture, whole blood, serum, plasma, swab, Bacteria, yeast, etc. It also features the ability to purify His-tag recombinant proteins. ExiPrep 16 Plus can handle up to 16 samples at once and includes optimized extraction protocols for many sample types so you get the unsurpassed results from your…
Bioneer’s AccuPower® PCR PreMix is a convenient lyophilized PCR master mix containing Top DNA Polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer. AccuPower PreMix PCR master mix includes our super-processive “three times faster than Taq” Top DNA Polymerase for the fastest nucleic acid amplification. While Top DNA Polymerase is ideal for applications where you would normally use Taq, if your needs include…
AccuPower® HotStart PCR PreMix is a PCR master mix containing a thermostable DNA polymerase, thermostable pyrophosphatase, reaction buffer, dNTPs, tracking dye, and patented stabilizer in a ready to use hotstart PCR master mix.Bioneer uses a unique enzyme-mediated HotStart PCR that provides robust and reliable results. Bioneer’s Top DNA Polymerase is inhibited at lower temperatures (
AccuPower® CycleScript RT (available with: dT20, dN12, or dN6 primers) PreMix is an easy to resuspend lyophilized PCR Master Mix of CycleScript Reverse Transcriptase, a primer, and all of the other components for cDNA synthesis conveniently packaged in individual tubes. Simply add template RNA, and water. Then, the reverse transcription is performed by either a Cyclic RT reaction (patent pending) or conventional reverse transc…
The AccuPower® RT PreMix is a master mix for cDNA synthesis that consists of an easy to resuspend, lyophilized mix of M-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, reaction buffer, tracking dye, and patented stabilizer. The master mix kit is used for first strand cDNA synthesis from RNA. Downstream applications include cDNA amplification with reverse transcription PCR. All of the key components are premixed at optimal c…
AccuPower® RT-PCR PreMix contains all the components necessary for sequential cDNA synthesis and amplification in one tube (one-step RT-PCR). This RT-PCR PreMix consists of both M-MLV Reverse Transcriptase, RNA dependent DNA polymerase, and a thermostable DNA polymerase in a lyophilized mix of dNTPs, reaction buffer, RNase inhibitor, tracking dye, and stabilizer. The kit can be used for double stranded cDNA synthesis from low…
Bioneer’s AccuPrep® Plasmid MiniPrep DNA Extraction Kit allows for the extraction of highly-pure plasmid DNA from as E. coli bacterial cultures. A260/A280 ratio greater than 1.8 and Yields of up to 20 μg of high-copy plasmid DNA can be routinely attained using the AccuPrep Plasmid MiniPrep DNA Extraction Kit. The AccuPrep Plasmid MiniPrep DNA Extraction Kit was developed for plasmid DNA purification from cultured bacterial cel…
Bioneer’s AccuPrep® PCR Purification Kit allows rapid purification of DNA fragments (dsDNA and ssDNA) from PCR products and other enzymatic reactions. Purification of up to 10 µg of DNA fragments from PCR and other enzymatic products within 5 minutes with > 90% recovery are routine with the AccuPrep PCR Purification Kit. Size range is 100 bp - 10 kb, so common 20 - 40 mer oligonucleotides are easily removed. Elution volume can…
Bioneer’s AccuPrep® Gel Purification Kit allows rapid DNA gel purification of up to 10 μg DNA fragments or plasmid DNA from low-melting, TAE and TBE agarose gels as well as PCR products and enzymatic reaction mixtures. Fragments 70 bp – 10 kb can be isolated in less than 15 minutes with the AccuPrep Gel Purification Kit Recovery efficiencies are QC’ed to be greater than 70%. Elution volume can be as little as 30 μl when concen…
Bioneer’s AccuPrep® GMO DNA Extraction Kit allows rapid DNA extraction from agricultural and food products to detect genetically modified organisms (GMOs). The AccuPrep GMO DNA Extraction Kit yields large amounts of high-purity DNA; for example, 20~40 μg of plant DNA for 1 g of powdered bean, with an average A260/A280 ratio greater than 1.8. AccuPrep GMO DNA Extraction Kit works on the principle that DNA, In the presence of ch…
Bioneer’s AccuPrep® Stool DNA Extraction Kit allows for the rapid extraction of genomic, bacterial, viral, and parasite DNA from fresh or frozen stool. The AccuPrep Stool DNA Extraction Kit yields large amounts of contaminant free, high quality DNA routinely with average A260/A280 ratio greater than 1.8.The AccuPrep Stool DNA Extraction Kit quickly and conveniently extracts up to 5μg of DNA from 100~200 mg of stool. In the pre…
Bioneer’s AccuPrep® Viral RNA Extraction Kit is the ideal solution for rapid extraction of viral RNA from cell-free samples such as serum, plasma, CSF, urine, and from a variety of RNA virus, such as HIV, HAV, HCV and enteroviruses. The viral RNA extracted with the AccuPrep Viral RNA Extraction Kit can be directly used as a template for RT or RT-PCR. AccuPrep Viral RNA Extraction Kit obtained has an average A260/A280 ratio gre…
Bioneer’s AccuPrep® 96 Plasmid DNA Extraction Kit is used for the high throughput extraction of plasmid DNA from 96 different bacterial culture samples. Bacterial cultures can be grown and lysed in the AccuPrep 96-well blocks provided with the Plasmid Extraction kit. Purification is accomplished with the BioVac 96 Vacuum Manifold. Average A260/A280 ratio greater than 1.8. Maximum yield of 17 μg.The DNA extraction protocol used…
Bioneer’s AccuPrep® 96 Genomic DNA Extraction Kit allows for the simultaneous genomic dna purification from 96 samples of cultured cells, whole blood, buffy coat, lymphocytes, plasma, serum, and other biological fluids. Yield is sample dependent, but is typically 6 μg per 200 µl healthy whole human blood. The AccuPrep 96 Genomic DNA Extraction Kit is based on the principle of DNA binding to a silica membrane in the presence of…
Bioneer’s AccuPrep® 96 PCR Purification Kit is a high throughput PCR purification kit that allows PCR product purification from up to 96 PCR reactions at once. The AccuPrep 96 PCR Purification Kit allows purification of up 10 μg of DNA fragments from PCR and other enzymatic products within 25 minutes. The AccuPrep 96 PCR Purification Kit features recovery efficiencies up to 95%. Size range is 100 bp - 10 kb, thus 20-40 mer oli…
Designed for use with the Bioneer AccuPrep® 96-well plate series - handles 96 samples simultaneously for high throughput DNA extraction. Simply attach to a vacuum pump for easy processing of all AccuPrep 96-well plate DNA extraction kits.
The Tissue RNA PreMate™ kit is designed for total RNA extraction from tissue, cultured cells, leukocytes or serum. The PreMate protocol is based on a Modified guanidinium salt-lysis method to optimally extract total RNA. Extracted total RNA is treated with PreMate’s RNase-free DNase to eliminate any genomic DNA. The RNA Isolated with the Tissue RNA PreMate kit is suitable for Northern analysis, dot blot hybridization, poly A s…
Viral RNA PreMate contains all of the required reagents for easy isolation of total RNA from RNA viruses such as HCV using the guanidinium salt-lysis method. Possible sources include tissue, cultured cells, leukocytes, and serum. The extracted RNA can be used for molecular cloning, RT-PCR, Northern analysis, dot blot hybridization, poly-A (+) selection, RNase protection assays, and other application that require pure RNA. Vira…
DNA PrepMate™ - M is used to extract genomic DNA for PCR from microorganisms with tough cell walls (such as Mycobacterium tuberculosis complex and Mycoplasma pneumoniae) using a standard microwave oven. DNA preparation is accomplished quickly and efficiently. Source materials can be sputum, CSF, urine, pleural fluid or tissue.
DNA PrepMate™ II uses an optimal binding buffer and silica matrix to effectively isolate DNA from bacterial cells, PCR products, gel fragments and enzymatic reaction mixtures. Examples include the purification of DNA fragments or plasmid DNA from low-melting, TAE and TBE agarose gel, high salt solutions, and lysed bacterial cells (using alkaline lysis). DNA preparation is accomplished quickly and efficiently. The size range fo…
