DNA PrepMate™ - M is used to extract genomic DNA for PCR from microorganisms with tough cell walls (such as Mycobacterium tuberculosis complex and Mycoplasma pneumoniae) using a standard microwave oven. DNA preparation is accomplished quickly and efficiently. Source materials can be sputum, CSF, urine, pleural fluid or tissue.
DNA PrepMate™ II uses an optimal binding buffer and silica matrix to effectively isolate DNA from bacterial cells, PCR products, gel fragments and enzymatic reaction mixtures. Examples include the purification of DNA fragments or plasmid DNA from low-melting, TAE and TBE agarose gel, high salt solutions, and lysed bacterial cells (using alkaline lysis). DNA preparation is accomplished quickly and efficiently. The size range fo…
Phosphoramidites for DNA oligonucleotide synthesis, gene synthesis, and more. Features of DNA Phosphoramidites: Amine functions are protected by either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. The recommended procedure for cleavage and deprotection for standard oligonucleotide synthesis is incubation in a concentrated ammonia solution for 8 hours at 55 °C. The purities of manufactured nucleoside phosphora…
Phosphoramidites for RNA oligonucleotides synthesis and more.Features of RNA Phosphoramidites: Amine groups are protected by “either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. Cleavage and deprotection procedures for RNA synthesis is similar to DNA synthesis, with just an additional step to remove the 2'-OH protecting group. The 2'-OH group is protected by a tert-butyldimethylsilyl (TBDMS) group to prevent de…
Amino linker phosphoramidites for the functionalization of the 5'-end during oligo synthesisFeatures• Amino linker products are coupled with standard synthesis protocols, identical to the coupling of DNA monomer phosphoramidites.• 5'-Amino phosphoramidite is used to add a functional amine group at the 5'- or 3'- end of the oligonucleotides. The primary amino group can be used for subsequent conjugation of a variety of repo…
Spacer phosphoramidites for oligonucleotide synthesis. Wide range of lengthsA wide selection of linker lengths available: C3, C4, C6 and C12Features• The spacer phosphoramidite are used to put spacer arms into oligonucleotides.C3, C6 and C12 spacer phosphoramidite contain an aliphatic linker which can be added to oligo requiring longer spacer arms. In general terms, spacer phosphoramidites are used during oligonucleotide s…
Phosphoramidites for nucleic acid labeling, microarray labeling, and moreFeatures• Oligonucleotides modified with fluorescein derivatives can be used for genomic research, molecular diagnostics and molecular biology.• t-butylamine/H2O/MeOH is recommended for cleavage and deprotection steps of TAMRA modified oligos.• dI-phosphoramidite can perform as an universal base as it hybridizes with any of the four bases.Technical Info…
High load CPG prepackaged Universal solid support for DNA SynthesisControlled Pore Glass (CPG) is the gold standard for solid phase oligonucleotide synthesis, with features such as high surface area, tightly controlled pore size and chemical inertness.Our High load CPG for DNA synthesis is available with or without phosphoramidites and can be purchased for standard synthesis sizes or for long-mer synthesis.Features• Utilizes C…
dNTP's from Bioneer are cost effective and hightly pure. Bioneer is one of the few manufacturers that actually produces our own phosphoramidites. Our dNTP's are made at our state-of-the-art manufacturing facilities, and Bioneer controls quality every step of the way in order to provide you with the best value for your research dollar.
Top DNA polymerase is a novel thermostable DNA polymerase that is more processive than Taq DNA polymerase. In fact, the extension rate of Top DNA Polymerase is > 3 x that of Taq DNA Polymerase! Top DNA Polymerase can be used for a variety of PCR applications (including TA cloning) and is a robust enzyme for everyday PCR. It contains no proofreading or 5’-3’ Exonuclease activity.Features and Benefits• Fast: More than three tim…
Get more cDNA in less time with CycleScript Reverse Transcriptase. CycleScript is a versatile reverse transcriptase - applicable to both conventional Reverse Transcription and Cyclic Reverse Transcription (Cyclic RT, patent pending – for cDNA amplification). It features high activity across a wide range of temperatures from 37 to 55°C; therefore, reverse transcription is carried out like PCR. The Cyclic RT reaction is composed…
Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase. This enzyme is able to use an RNA molecule as a template and synthesize a double-strand DNA. M-MLV Reverse Transcriptase is isolated from a E.coli strain containing a recombinant clone. It is ideal for use of first-strand synthesis cDNA from RNA molecules and for cDNA synthesis for Reverse Transcriptase PCR and qRT-PCR.
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will ligate DNA from blunt-end and cohesive-end termini (Including sticky ends for TA Cloning) as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids. T4 DNA Ligase is isolated from a recombinant E.coli strain.
Bioneer is pleased to offer our Custom Gene synthesis service to help customers save time and money while improving research results. Gene synthesis is the most cost effective way to enhance your research. In as little as 2 weeks, you can have your Gene cloned in hand and 100% sequence guaranteed. And with Bioneer’s great pricing it can cost less to order a synthetic gene from us than to buy all the kits and reagents necessary…
Gene cloning and subcloning of genes into any cloning vector you want - 100% sequence guaranteed.
The fastest and most cost effective way to construct a mutant library for protein structure and function research or to increase enzyme function is to use our Point Mutation Service combined with our Gene Synthesis Service. Site-directed mutation such as Point Mutation as well as deletion mutation services are available. For unsurpassed quality and value pricing, partner with Bioneer today!
25/100 bp Mixed DNA Ladder is designed for determining the size of double stranded DNA from 25 to 2,000 bp. The ladder consists of 17 double stranded DNA fragments ranging in size from 25 to 200 bp in 25 bp increments, from 200 to 1,000 bp in 100 bp increments, and an additional fragment of 2,000 bp. The DNA marker fragments at 150, 500, 1,000 and 2,000 bp bands are two times brighter for easy identification.
25/100 bp Mixed DNA Ladder is designed for determining the size of double stranded DNA from 25 to 2,000 bp. The ladder consists of 17 double stranded DNA fragments ranging in size from 25 to 200 bp in 25 bp increments, from 200 to 1,000 bp in 100 bp increments, and an additional fragment of 2,000 bp. The DNA marker fragments at 150, 500, 1,000 and 2,000 bp bands are two times brighter for easy identification.
100 bp DNA Ladder was designed to determine the size of double stranded DNA fragments from 100 to 2,000 bp. The 100 bp DNA Ladder consists of 13 double stranded molecular weight markers ranging in sizes from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000 bp. The 500, 1,000 and 2,000 bp bands are two times brighter for easy identification.
100 bp DNA Ladder was designed to determine the size of double stranded DNA fragments from 100 to 2,000 bp. The 100 bp DNA Ladder consists of 13 double stranded molecular weight markers ranging in sizes from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000 bp. The 500, 1,000 and 2,000 bp bands are two times brighter for easy identification.
100 bp Plus DNA Ladder was designed to determine the size of double stranded DNA fragments from 100 to 10,200 bp. The 100 bp Plus DNA Ladder consists of 19 double stranded molecular weight markers, ranging in size from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000, 2,961, 4,000, 5,007, 5,991, 8,000, 10,200 bp. The 200, 1,000 and 2,000 bp bands are two times brighter for easy identificati…
100 bp Plus DNA Ladder was designed to determine the size of double stranded DNA fragments from 100 to 10,200 bp. The 100 bp Plus DNA Ladder consists of 19 double stranded molecular weight markers, ranging in size from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000, 2,961, 4,000, 5,007, 5,991, 8,000, 10,200 bp. The 200, 1,000 and 2,000 bp bands are two times brighter for easy identificati…
1 kb DNA Ladder is designed for determining the size of double stranded DNA ranging between 500 to 10,000 bp. The 1 kb Ladder consists of DNA fragments 0.5, 1.0, 1.6, 2.0, 3 (2.961), 4, 5 (5.007), 6 (5.991), 8 and 10 (10.2) kb. The 2.961 kb band is approximately two times brighter for easy identification.
1 kb DNA Ladder is designed for determining the size of double stranded DNA ranging between 500 to 10,000 bp. The 1 kb Ladder consists of DNA fragments 0.5, 1.0, 1.6, 2.0, 3 (2.961), 4, 5 (5.007), 6 (5.991), 8 and 10 (10.2) kb. The 2.961 kb band is approximately two times brighter for easy identification.
Lambda DNA/EcoR I Marker is created by digesting lambda DNA with the restriction enzyme EcoR I. The resulting DNA ladder is then purified and buffer and dye are added so the DNA marker is ready to load. Lambda DNA/EcoR I Marker consist of 6 double stranded lambda DNA fragments ranging in size from 3,530 to 21,226 bp.
