Diagenode has revolutionized immunoprecipitation with a novel, rigorous antibody validation system. We have partnered with epigenetics experts including the BLUEPRINT Epigenome consortium to develop the absolute best epigenetics antibodies with the highest specificity and sensitivity available. These Diagenode Validated Antibodies are categorized into three different classes (Premium, Classic, and Pioneer) dependent on their…
Chromatin immunoprecipitation and DNA methylation assays followed by next generation sequencing is a powerful technique for generating genome-wide profiles of epigenetic modifications and transcription factor binding sites. The SX-8G IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental conditions. Samples generated on the system are…
Introducing Diagenode’s latest innovation – a superior method for extracting DNA from FFPE samples. The FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin, digest tissues, and purify DNA with high yields and low sample degradation. The DNA can then be analyzed by tradtional methods or can be sheared with the Bioruptor® Pico ultrasonicator for downstream next g…
Diagenode's High Resolution Library Preparation Kit significantly enhances the resolution of transcription factor binding sites to single nucleotide levels.Using an optimized, proprietary protocol using an exonuclease to digest protein-bound DNA from the 5' end to within a few nucleotides of the protein binding site, the precise location of transcription factor-bound DNA can be determined. The High Resolution Library Preparati…
The iDeal Library Preparation Kit reliably converts DNA into indexed libraries for next-generation sequencing, with input amounts down to 5 ng.Our kit offers a simple and fast workflow, high yields, and ready-to-sequence DNA on the Illumina platform. Combine this kit with our iDeal ChIP-seq kit for optimal ChIP-seq results. For library preparation of DNA inputs down to 50 pg, our MicroPlex Library Preparation Kit is an optimal…
A breakthrough in genome engineering. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate up- or downregulation of specific endogenous genes or to alter hi…
The ChIPettor™ System has everything you need for high-quality, fast, and easy chromatin immunoprecipitation for either histones or transcription factor ChIP. Contains a full set of reagents for start-to-finish ChIP starting with 100,000 to 1,000,000 cells (ideal for histone pull down), purification reagents, and a semi-automated pipettor with special resin pipet tips. The ChIPettor™ is designed to stand independently on a 9…
Diagenode’s Premium RRBS Kit for reduced representation bisulfite sequencing.
ChIP, ChIP-seq, and methyl antibodies for stem cell studies. In recent years, studies suggest that the mammalian genome undergoes various epigenetic modifications during development and embryonic stem cell (ESC) differentiation. Understanding the mechanisms that control these epigenetic signals is essential in deciphering stem cell biology and the patterns of gene expression during cellular differentiation. ESC gene express…
The iDeal ChIP Kit for Transcription Factors. Diagenode’s iDeal ChIP-seq Kit for Transcriptions Factors is a highly specialized solution for robust ChIP-seq results transcription factors. Unlike competing solutions, our kit utilizes a highly optimized protocol and is backed by validation with a broad number and range of transcription factors. The kit provides high yields with excellent specificity and sensitivity. Adv…
