The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. An excellent device for shearing chromatin or DNA, cell and tissue disruption and many other applications. Diagenode’s Bioruptor® Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and othe…
The Diagenode’s LowCell# ChIP kit is the only complete magnetic bead based ChIP (Chromatin Immunoprecipitation) kit that can be used with as low as 1000 - 10 000 cells per IP. Ideal for Stem cells, embryos and biopsies. Use from 1,000 - 100,000 cells per IP Improved handling and reproducibility by the use of magnetic beads and special Magnetic rack (0.2 ml tubes) Optimized purification and de-crosslinking step One…
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Its conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.
pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay.
The Bioruptor® Pico is the new next-generation all-in-one shearing device, highly optimized for DNA shearing for next-generation sequencing, chromatin shearing for ChIP-seq applications, RNA shearing, protein extraction, and cell/tissue lysis. The Pico is the shearing device of choice for sequencing applications, providing optimal yields, lengths, and consistency and excels in shearing different fragment size ranges, which…
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This microChIP-seq assay has been validated with the new generation IP-Star® Compact Automated Workstation. Furthermore Diagenode's high quality ChIP-seq grade antibodies have been used. Features: Revolutionary: Only 10.000 cells needed for complete ChIP-seq procedure Relia…
The easiest NGS Library preparation you will ever find! The MicroPlex Library Preparation™ kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the True MicrochIP kit, it allows for performing ChIP-seq on as few as 10,000 cells. Studies showed a perfect match between the top 40% of peaks and the reference ChIP-…
Diagenode’s ultimate solution for long fragment library preparation between 2kb and 75kb– the Megaruptor® fragmentation system. A number of applications for massively parallel sequencing, also known as Next-Generation Sequencing, require libraries of long DNA segments, but few methods have been available for reliably generating fragments greater than 2 kb. The Megaruptor, which has been used by several sequencing centers w…
The iDeal ChIP Kit for Transcription Factors.Diagenode’s iDeal ChIP-seq Kit for Transcriptions Factors is a highly specialized solution for robust ChIP-seq results transcription factors. Unlike competing solutions, our kit utilizes a highly optimized protocol and is backed by validation with a broad number and range of transcription factors. The kit provides high yields with excellent specificity and sensitivity. Advantages ov…
The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methylbinding domain) protein . This procedure has first been optimized on the SX-8G IP-Star Automated System ([page product SX-8G IP-Star]. Afterwards, this method has been adapted to a manual protocol, which has been inspired by the same reproducibility standards provided by the automated MethylCap assay. Allows for fractionati…
Diagenode has revolutionized immunoprecipitation with a novel, rigorous antibody validation system. We have partnered with epigenetics experts including the BLUEPRINT Epigenome consortium to develop the absolute best epigenetics antibodies with the highest specificity and sensitivity available. These Diagenode Validated Antibodies are categorized into three different classes (Premium, Classic, and Pioneer) dependent on their…
Chromatin immunoprecipitation and DNA methylation assays followed by next generation sequencing is a powerful technique for generating genome-wide profiles of epigenetic modifications and transcription factor binding sites. The SX-8G IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental conditions. Samples generated on the system are…
Introducing Diagenode’s latest innovation – a superior method for extracting DNA from FFPE samples. The FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin, digest tissues, and purify DNA with high yields and low sample degradation. The DNA can then be analyzed by tradtional methods or can be sheared with the Bioruptor® Pico ultrasonicator for downstream next g…
Diagenode's High Resolution Library Preparation Kit significantly enhances the resolution of transcription factor binding sites to single nucleotide levels.Using an optimized, proprietary protocol using an exonuclease to digest protein-bound DNA from the 5' end to within a few nucleotides of the protein binding site, the precise location of transcription factor-bound DNA can be determined. The High Resolution Library Preparati…
The iDeal Library Preparation Kit reliably converts DNA into indexed libraries for next-generation sequencing, with input amounts down to 5 ng.Our kit offers a simple and fast workflow, high yields, and ready-to-sequence DNA on the Illumina platform. Combine this kit with our iDeal ChIP-seq kit for optimal ChIP-seq results. For library preparation of DNA inputs down to 50 pg, our MicroPlex Library Preparation Kit is an optimal…
A breakthrough in genome engineering. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate up- or downregulation of specific endogenous genes or to alter hi…
The ChIPettor™ System has everything you need for high-quality, fast, and easy chromatin immunoprecipitation for either histones or transcription factor ChIP. Contains a full set of reagents for start-to-finish ChIP starting with 100,000 to 1,000,000 cells (ideal for histone pull down), purification reagents, and a semi-automated pipettor with special resin pipet tips. The ChIPettor™ is designed to stand independently on a 9…
Diagenode’s Premium RRBS Kit for reduced representation bisulfite sequencing.
ChIP, ChIP-seq, and methyl antibodies for stem cell studies. In recent years, studies suggest that the mammalian genome undergoes various epigenetic modifications during development and embryonic stem cell (ESC) differentiation. Understanding the mechanisms that control these epigenetic signals is essential in deciphering stem cell biology and the patterns of gene expression during cellular differentiation. ESC gene express…
The iDeal ChIP Kit for Transcription Factors. Diagenode’s iDeal ChIP-seq Kit for Transcriptions Factors is a highly specialized solution for robust ChIP-seq results transcription factors. Unlike competing solutions, our kit utilizes a highly optimized protocol and is backed by validation with a broad number and range of transcription factors. The kit provides high yields with excellent specificity and sensitivity. Adv…
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit for Histones has everything you need for a successful start-to-finish ChIP of histones prior to Next-Generation Sequencing. The complete kit contains all buffers and reagents for cell lysis, chromatin shearing, immunoprecipitation and DNA purification. In addition, unlike competing solutions, the kit contains positive and ne…
Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The TAG Kit for ChIPmentation has been developed for researchers who would like to perform Ch…
Cas9 Nuclease protein NLS, in complex with guide RNA, offers benefits for CRISPR/Cas9 technology over plasmid or mRNA transfection: Off-target mutations rarely occur Prolonged expression of Cas9 increases off-target editing but RNP delivery is naturally transient Transfection/injection is easy Cas9-NLS bypasses transcription and translation by delivering the protein directly The efficiency of the guide RNA in vit…
Perform MeDIP (Methylated DNA Immunoprecipitation) followed by qPCR to estimate DNA methylation status of your sample using 5-methylcytosine antibody. Our kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol. Characteristics Generate highly consistent results with internal controls in 24h Minimize error with many reagents in 1 tube Optimized pu…
Perform MeDIP (Methylated DNA Immunoprecipitation) followed by sequencing to get DNA methylation status of your sample using 5-methylcytosine antibody and magnetic beads. Our kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol. This package contains everything you need from gDNA extraction to library preparation and quality check. It allows you to perfo…
