Cell biology is reliant on the investigation of unmanipulated naturally occurring cells. State-of-the-art receptor specific isolation procedures, however, leave binding reagents on the isolated cells which is likely to induce unwanted cell modifying signalling events. This can now be prevented by using a novel purification approach called Streptamer® technology. Mild purification conditions at low temperatures along with a qui…
The Strep-tag® / Strep-Tactin® purification system allows the isolation of above 95% pure and functional proteins after one chromatographical step. The Strep-tag system is based on the highly selective and easily controllable interaction between the Strep-tag II peptide and the specially engineered streptavidin called Strep-Tactin. The binding affinity of Strep-tag II to Strep-Tactin is nearly 100 times higher than to Stre…
FasL-Strep, a stable homotrimer, efficiently induces the CD95 pathway of apoptosis and is highly suited for evaluation of CD95 (Fas) receptor-ligand interaction. Human or murine FasL-Strep (CD95L-T4) is an engineered human or murine Fas (CD95) ligand. C-terminal fusion of the T4-FOLDON to the extracellular FasL (CD95L) receptor-binding domain (E142-L281) results in bioactive, conformationally stabilized, well defined trimers…
The recently launched Strep-Tactin® Superflow® high capacity resin offers improved binding capacity but identical high protein purity compared to standard Strep-Tactin® Superflow®. Thus, it is the perfect solution for Strep-tag® protein purification. Both Strep-Tactin® Superflow® resins • combine outstanding flow characteristics with superior mechanical stability • are suitable for increased flow rates and useful for purificat…
Cell biology is reliant on the investigation of unmanipulated naturally occurring cells. State-of-the-art receptor specific isolation procedures, however, leave binding reagents on the isolated cells which is likely to induce unwanted cell modifying signalling events. This can now be prevented by using a novel purification approach called Streptamer® technology. Mild purification conditions at low temperatures along with a q…
Strep-Tactin Superflow combines superior mechanical stability and outstanding flow characteristics with high dynamic binding capacity. It is suitable for increased flow rates and is also useful for the purification of large protein complexes. The Strep-tag® / Strep-Tactin® purification system allows the isolation of above 95% pure and functional proteins after one chromatographical step. The Strep-tag system is based on the…
Strep-tag Protein Purification Buffer Set; convenient buffer set for the purification of recombinant Strep-tag fusion proteins on immobilized Strep-Tactin and its regeneration. Buffer W (washing buffer, 10x, 100 ml), Buffer E (elution buffer, 10x, 25 ml), Buffer R (regeneration buffer, 10x, 100 ml).
MATra-si Reagent for efficient transfection of siRNA/miRNA as well as Antisense Oligos (ODN) using IBA's Magnet Assisted Transfection technology (MATra). MATra is a very gentle, easy-to-handle, fast and highly efficient technology to transfect cells in culture. Data from a variety of species using cell lines or primary tissue culture have accumulated including human, monkey, mouse, rat, xenopus, pig, cat or fish. Applications…
MATra-A Reagent for efficient transfection of plasmid DNA using IBA's Magnet Assisted Transfection technology (MATra). MATra is a very gentle, easy-to-handle, fast and highly efficient technology to transfect cells in culture. All types of nucleic acids from plasmid DNA or siRNA to oligonucleotides can be used with the MATra approach. Data from a variety of species using cell lines or primary tissue culture have accumulated in…
Test set for comparison of Magnet Assisted Transfection versus Magnet Assisted Lipofection. Including a one-month-rent of a universal magnet plate. Optimized for siRNA transfections. Magnet Assisted Transfection (MATra) is a new, easy-to-handle, very fast and highly efficient technology to transfect cells in culture. Data from a variety of species using cell lines or primary tissue culture have accumulated including human, mon…
