The OxyBlot™ Protein Oxidation Detection Kit provides the chemical and immunological reagents necessary to perform the immunoblot detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation.
OxyBlot™ Protein Standard is composed of five proteins (phosphorylase B, BSA, ovalbumin, carbonic anhydrase, trypsin inhibitor) containing 1-3 dinitrophenylhydrazone (DNP) residues per protein. These attached DNP moieties permit the visualization of the proteins by the detection system of the OxyBlot™ Protein Oxidation Detection Kit. OxyBlot™ Protein Standard serves as an internal control for the electrophoresis, western trans…
Streptavidin isolated from Streptomyces avidinii covalently bound to agarose.
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been…
The IHC Select® Immunoperoxidase Secondary Detection System is intended for use with the CHEMICON IHC Select® Prediluted Primary Antibody reagents, which contain rabbit and mouse IgG for the qualitative identification of antigens by light microscopy in paraffin-embedded tissues. The IHC Select® Immunoperoxidase Secondary Detection System may also be used with other rabbit or mouse primary antibodies. The IHC Select® Immunopero…
Live cell tracking of single cell or cell population migrationEstablishes a stable and linear concentration gradient that lasts ≥ 48 hoursHelps distinguish chemotaxis from random movementAllows for multiparametric analysis, including cell directionality and velocityEnhanced optical imaging of slow- and fast-migrating cellsAnalyze 3 chemoattractants in parallel for increased throughput and flexibilityNo need to spend time or mo…
ECGS is mitogenic for many types of cells under reduced or serum-free conditions, such as mammalian, avian and human endothelial cells, smooth muscle cells, keratinocytes, melanocytes and hybridomas; ECGS often fully substitutes for feeder layers in culture of fastidious cells.
EmbryoMax® 1X Dulbecco's Phosphate Buffered Saline w/o Ca++ & Mg++
EmbryoMax® 10X Dulbecco's Phosphate Buffered Saline w/o Ca++ & Mg++
Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. Xu, et al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proli…
The Mammalian Cell Transfection Kit is an optimized system suitable for the introduction of DNA into mammalian cells. DNA is introduced to the cells as a calcium phosphate precipitate. Transfected cells integrate and express the exogenous DNA at a higher efficiency, producing stable clones of the specifically altered genotype and phenotype. This system has been used to introduce various plasmids into a broad range of cell line…
Enzyme-Free Cell Dissociation Solution is a Hank's based formulation of chelating agents and agents to stabilize their activity on cells. This reagent does NOT contain porcine trypsin or any other protein. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cyto…
Enzyme-Free Cell Dissociation Solution is a PBS based formulation of chelating agents and agents to stabilize their activity on cells. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cytotoxic effects sometimes associated with 0.5 mM EDTA. It can be used to…
ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell VM was derived from the ventral mesencephalon region of human fetal brain. Immortalized by retroviral transduction with the v-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell VM retain…
EndoGRO Human Umbilical Vein Endothelial Cells (HUVEC) are a commonly studied human cell type extracted from human neonatal umbilical cords. They are relatively easy to culture, and provide a valuable cell model for many vascular biology research applications, including inflammation, angiogenesis, atherosclerosis, blood clotting, vasoconstriction, and vasodilation. EndoGRO™ HUVEC are provided at passage 1, and have been qualit…
ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture and expansion of ENStem-A Human Neural Progenitor Cells. When used in conjunction with L-Glutamine (not provided) and FGF-2 (provided), the expansion medium will allow for the maintenance and proliferation of ENStem-ATM Human Neural Progenitor Cells.
ReNcell NSC Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will…
ReNcell Neural Stem Cell Freezing Medium is qualified for use with ReNcell immortalized human neural progenitor cell lines, CX and VM cultured in serum-free conditions with ReNcell NSC Maintenance Medium. The optimized formulation allows for consistent cryopreservation and high viability upon thawing and plating.
ENStem-A™ Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A™ human neural progenitor cells to a neuronal lineage. The medium has been extensively validated on ENStem-A™ Human Neural Progenitor Cells. Using ENStem-A™ Neuronal Differentiation Medium, relatively pure populations of neuronal cells (90% bIII-tubulin positive) are obtained. Very low levels of…
HEScGRO hES cell medium is the first, animal-component free medium that is specially formulated to meet the unique requirements of human embryonic stem cell culture. No other hES cell media is available as a serum-free, ready-to-use, and complete formulation. HEScGRO has been extensively tested and proven to maintain the pluripotential nature of several hES cell lines including: MEL-1, MEL-2 and H1. This medium is fully-define…
A cell detachment solution of proteolytic, collagenolytic and DNAse enzymes.ACCUMAX™ is tested for Sterility, enzymatic activity and cell detachment from tissue culture plastic. ACCUMAX™ has been used successfully on numerous cell types including: CHO, BHK, 293, COS, Sf9, and catfish primary hepatocyte cells.
This cell line arose from a primary culture of dispersed human fetal pancreatic cells. A single pancreas gland at 18 weeks gestation was dispersed by micro-dissection and collagenase digestion (2 mg/ml for 50 minutes at 37°C). Following washout of enzyme, dispersed cells were incubated with magnetic beads coupled to monoclonal antibodies specific to CD90 though a DNA linkage. Magnetic isolation and the detachment of the magnet…
Embryonic Stem Cell Lines derived from murine 129/SVEV selected for high targeting efficiency and proven germ line transmission. Cells are supplied as 2.5 x 106 ES cells per vial (approximately 2.5 population doublings per passage, shipped frozen on dry ice) and are supplied at passage 11.
Embryonic Stem Cell Lines derived from murine strain C57/BL6J selected for high targeting efficiency and proven germ line transmission. Cells are supplied as 2.5 x 106 ES cells per vial (approximately 2.5 population doublings per passage, shipped frozen on dry ice) and are supplied at passage 11.
