LT2 Immortalized Pancreatic Mesenchymal Cell Line
This cell line arose from a primary culture of dispersed human fetal pancreatic cells. A single pancreas gland at 18 weeks gestation was dispersed by micro-dissection and collagenase digestion (2 mg/ml for 50 minutes at 37°C). Following washout of enzyme, dispersed cells were incubated with magnetic beads coupled to monoclonal antibodies specific to CD90 though a DNA linkage. Magnetic isolation and the detachment of the magnet…
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This cell line arose from a primary culture of dispersed human fetal pancreatic cells. A single pancreas gland at 18 weeks gestation was dispersed by micro-dissection and collagenase digestion (2 mg/ml for 50 minutes at 37°C). Following washout of enzyme, dispersed cells were incubated with magnetic beads coupled to monoclonal antibodies specific to CD90 though a DNA linkage. Magnetic isolation and the detachment of the magnetic beads by DNAse treatment resulted in the separation of fibroblasts from epithelial cells. The CD90 positive cells were then cultured in Pancreatic Cell Culture Supplement (SCR015) and transfected with an expression vector containing the large T antigen sequence. Antibiotic selection was used to select transfectants.
