Products & Reviews

ChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP…

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The quality of sequence obtained from Next Generation sequencing platforms such as Illumina, Inc. requires the accurate determination of input library DNA. Loading too much DNA can lead to overlapping clusters and loss of read counts whereas too little input can lead to poor read lengths and lower sequence output. The PureGenome™ NGS Library Validator Kit measures the absolute amount of adapter-ligated, sequence ready DNA in I…

The PureGenome™ On-Spot Tissue DNA kit allows for convenient isolation of genomic DNA in any targeted microscopic tissue area on a slide. The system combines a powerful area-specific tissue sampling technology with a one-step DNA extraction method and makes targeted tissue genomic DNA isolation easy and simple. The kit efficiently recovers genomic DNA from paraffin archives or fresh tissue sections for PCR analysis. Unlike a c…

The PureGenome™ Tissue DNA Extraction Kit is a simple procedure for the rapid isolation of total DNA (e.g. genomic, mitochondrial, parasitic, microbial, viral) from a variety of soft and solid tissues, cells and a range of biological liquids. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is also compatible with buffy coat, bone marrow, cells from culture, whole blood (fres…

A sterile, closed, disposable system for sampling fluids from sterile and aseptic processes The unique NovaSeptum sampling system is ideal for general fluid and cell culture sampling from aseptic and sterile processes. Both systems can be configured for high purity or autoclavable sampling applications and as single or multiple sampling units. A wide range of holders and connectors are also available. •  Closed sampling elimin…

GeneJuice® Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity. This transfection reagent is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, and complex formation with DEAE-dextran. Whereas many available transfection reagents…

Pellet Paint® NF Co-Precipitant* is a non-fluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye™ Terminators (PE Corp.) during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with centrifugation times of 10 minutes. The easily visualized carrier provides a simple confirmation that precipitation has o…

Rapid, quantitative precipitation of DNA and RNA, including PCR clean-upPellet Paint® Co-Precipitant* is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids (1, 2). The two minute precipitation requires no low temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easil…

Overnight Express™ Instant TB Medium is a complete granulated culture medium for high-level protein production without the need to monitor cell growth in the pET and other IPTG-inducible bacterial expression systems. Cell mass and target protein yield are often increased several-fold as compared with induction using IPTG. The method is based on media components that are metabolized differentially to promote growth to high dens…

White solid. Aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3¡ä-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Intro…

Light brown lyophilized solid. Working concentration 50 mg/ml - 1 mg/ml for mammalian cell selection. Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isol…

The ProteoPlex™ 16-Well Murine Cytokine Array Kit is designed for multiplex detection and measurement of 10 important mouse cytokines in parallel from up to 15 experimental samples. The 10 analytes measured in the kit are pro- and anti- inflammatory cytokines important for the study of immune system regulation and diseases such as asthma and arthritis. Each well on the slide contains a microarray of spotted antibodies with fou…

Novel protein markers for visible tracking & accurate sizing on any Western blotTrail Mix™ Western Markers consist of the Perfect Protein™ Western Markers supplemented with a group of three prestained indicator proteins to allow direct visualization of protein mobility during electrophoresis. Trail Mix Western Markers and Western Blot Kits have all of the features of Perfect Protein Western Markers and Western Blot Kits, plus…

The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of Novagen's expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10 to 8–1 × 10–9 M) enables sensitive, specific detection of His•Tag fusion prote…

Benzonase Nuclease - Effective viscosity reduction and removal of nucleic acids from protein solutions Benzonase Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. Benzonase Nuclease is effective over a wide range of conditions and possesses an exceptionally high specif…

The most common method for transformation of plasmids into E. coli is to use chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility and convenience are achieved using prepared competent cells. Novagen offers the widest selection of competent cells available for protein expression. Our strains offer a variety of options that enable you to maximize the yield an…

Novabiochem® building blocks for routine peptide synthesis applications

Pseudoproline dipeptides are undoubtedly the most powerful tools described to date for enhancing synthetic efficiency in Fmoc SPPS. Novabiochem® now offers 28 pseudoproline dipeptides - representing the widest selection on the market today The routine use of pseudoproline dipeptides has been found to: Avoid costly and unnecessary repeat syntheses of failed sequences Increase purity of crude products Simplify HPLC puri…

Calbiochem® supplies a wide variety of products for the study of apoptosis, includingActivators and Inhibitors Antibodies and Blocking Peptides Assay Kits Caspase-related products Mitochondrial Permeability-Transition Reagents and Related Products Poly(ADP-Ribose) Glycohydrolase (PARG) Inhibitors Poly(ADP-Ribose) Polymerase (PARP) Inhibitors Telomerase Products View the online interactive pathways to easily locate the product…

The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Based on the T7 promoter-driven system, Novagen’s pET System has been used to express thousands of different proteins. pET Vectors are supplied as purified plasmid DNA (10 µg) plus an Induction Control strain (supplied as a glycerol stock). Complete sequences and detailed vector maps are available.

The D-Tube™ Dialyzers can be used for dialysis and electroelution of proteins or nucleic acids from polyacrylamide or agarose gels. The disposable, single-use tubes require no syringes, microcentrifuges, or laborious steps to manipulate small samples volumes. The sample is added and removed using a standard laboratory pipette. Available with molecular weight cut-offs from 3.5 to 14 kDa, the D-Tube Dialyzers are designed in thr…

Quick and Easy Antibody Quantitation:With its simplicity and ease of use, Guava’s new RapidQuantTM IgG Kits have raised the bar for IgG titering assays. Designed to be time-saving, labor-saving and money-saving, Guava’s RapidQuant kits eliminate the most tedious, laborious portions of antibody titering methods with Guava’s signature ease of use. • Robust, rapid and highly reproducible • Relevant quantitative range • Quantifies…