Competent Cells

The most common method for transformation of plasmids into E. coli is to use chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility and convenience are achieved using prepared competent cells. Novagen offers the widest selection of competent cells available for protein expression.

Our strains offer a variety of options that enable you to maximize the yield and activity of your target proteins, including stringent control over basal expression levels, the ability to form disulfide bonds in the cytoplasm for proper folding and activity, and alleviating codon usage incompatibilities.

Every strain of Novagen's competent cells is verified for phenotype and purity, and transformation efficiency is guaranteed. The standard aliquot is 0.2 ml, which provides enough cells for 10 transformations; pack sizes are 2 × 0.2 ml and 5 × 0.2 ml. Several strains are also available as Singles™ Competent Cells, which are provided in single-use 50-µl aliquots for extra convenience and efficiency. For the ultimate in convenience and throughput, the NovaBlue and BL21(DE3) strains are also available as HT96™ configurations, which consists of pre-dispensed 20-µl aliquots in an automation-compatible 96-well format.

Features

• Guaranteed efficiency for consistent, reliable results
  
• Test Plasmid and SOC Medium included20-µl standard
  
• transformation volume for economy
  
• Available in standard 0.2-ml aliquots
  
• Certain strains available as Singles™ and HT96™ Formats
  
• Widest selection of protein expression strains available