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Products, services, reviews and techniques used in lead molecule delivery, formulation and toxicology, using assays and animal models.

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Apo-Direct TUNEL Assay Kit

Merck

The CHEMICON APO-DIRECT™ Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.

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MitoLight® Mitochondrial Apoptosis Detection Kit

Merck

The MitoLight™ Apoptosis Detection Kit utilizes a lipophilic cation, termed as MitoLight™, as a mitochondrial activity marker. MitoLight™ is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. The monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential. MitoLight™ partitions differently i…

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Cell Transformation Detection Assay

Merck

CHEMICON®'s Cell Transformation Detection Assay is an anchorage-independent growth assay in soft agar, which is considered the most stringent assay for detecting malignant transformation of cells. For this assay, cells (pre-treated with carcinogens or carcinogen inhibitors) are cultured with appropriate controls in soft agar medium for 21-28 days. Following this incubation period, formed colonies can either be analyzed morphol…

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Mast Cell Degranulation Assay Kit

Merck

Chemicon's Mast Cell Degranulation Assay Kit provides a simple and convenient means for assaying tryptase activity. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate tosyl-gly-pro-lys-pNA. The free pNA can then be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. A pNA Standard is provided as a comparison control.

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