Rat/Mouse Growth Hormone ELISA
MerckGrowth Hormone (GH) is a 23 kDa circulating pituitary hormone, Rat GH shares approximately 97% homology with mouse GH and 65% homology with human GH in its protein sequence.
Growth Hormone (GH) is a 23 kDa circulating pituitary hormone, Rat GH shares approximately 97% homology with mouse GH and 65% homology with human GH in its protein sequence.
GIP, also known as gastric inhibitory polypeptide, or glucose-dependent insulinotropic polypeptide, is a 42 amino acid peptide hormone synthesized in and secreted from K cells in the intestinal epithelium.
Insulin, a 5.8 kDa hormone, is secreted by the islet beta cells in the pancreas.
The OxyELISA™ Oxidized Protein Quantitation Kit provides a sensitive methodology for detection and quantitation of proteins modified by oxygen free radicals and other reactive species. Oxygen-derived free radicals have been implicated in numerous physiological functions such as apoptosis, cancer, aging, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and cardiovascular diseases (for reviews, see…
TMB, (3,3',5,5'-tetramethybenzidine) has been shown to be a safe-sensitive substrate for the assay of HRP. Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum of 653 nm. Further reaction with HRP/H2O2 or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing…
Sterivac vacuum-driven filter units are ideal for bottle-top filtering directly into receiver flasks at flow rates as fast as 1.5 L/min. The unique "start & stop" filtration action makes setting up, filtering and transferring the unit to another receiver fast and easy. Push a button to stop filtering, move the device to an empty bottle and restart
• Concentrating and desaltingchromatography column eluates• Concentrating monoclonal antibodies• Concentrating proteins or viruses from culture supernatants• Clarifying tissue homogenates and cell lysates• Buffer exchange or diafiltration
High recovery Durapore (PVDF) and hydrophilic PTFE membranesFive different pore sizes from 0.1 to 5.0 µmPre-sterilized units also availableFast filtration and highly reproducible performanceUse in fixed-angle rotors for 15 mL tubes
Easy, pre-sterilized, centrifugal sample clarification units for either 0.5 mL (MC) or 2 mL (CL) maximum volumes.High recovery Durapore (PVDF) membraneFast filtration and highly reproducible performanceUse in fixed-angle rotors for 1.5 mL tubes (MC) or 15 mL tubes (CL)
Montage Antibody Purification Kits are designed for fast and easy antibody purification from serum, ascites or cell culture supernatants. The kits include spin columns pre-packed with high capacity PROSEP-A or G media. There are no pumps to setup. No columns to pack. All you need is a centrifuge to purify 10–20 mg in less than 60 minutes.The protocol is easy to follow and, unlike traditional gravity flow methods, the use of ce…
Concentrates 2 mL down to 15-70 µL 98% sample recovery Fast processing time - 10 to 30 minute spin times Engineered dead stop Reverse spin capability provides consistent recoveries
Automation compatible filter units from EMD Millipore are specially designed to provide efficient filtration for dissolution and other automated sample preparation applications.Choice of Filter Media: Automation compatible filter units are available in a variety of membrane types and pore sizes, including units with prefilters for particulate-laden samples.Automation Compatible Packaging: The 200 pack comes preassembled in 8 t…
Millex filter units with hydrophobic Fluoropore membrane are ideal for sterilizing gases, venting sterile containers, and sterilizing or clarifying organic solutions. Specialized filter units protect hemodialysis transducers from blood and moisture. 50 mm Millex filter units are also used for vacuum line protection. All units are bi-directional
This 13 mm syringe filter with a Hydrophilic Polyethersulfone membrane, for non-sterile filtration, has a 0.45 micron pore size used in preparation of aqueous & mild organic solutions prior to chromatography or other instrument analyses.
Millex-GP Filter for Samplicity G2, 0.22 µm, PES 33 mm, non-sterile
The Luminata ELISA HRP chemiluminescent substrates provide three major advantages:High sensitivity for detecting as little as 0.1 pg of proteinBroad dynamic detection range (up to 2,500-fold), enabling a wide range of quantitation.Flexibility to adjust ELISA signal strength
Calbiochem® supplies a wide variety of products for the study of apoptosis, includingActivators and Inhibitors Antibodies and Blocking Peptides Assay Kits Caspase-related products Mitochondrial Permeability-Transition Reagents and Related Products Poly(ADP-Ribose) Glycohydrolase (PARG) Inhibitors Poly(ADP-Ribose) Polymerase (PARP) Inhibitors Telomerase Products View the online interactive pathways to easily locate the product…
The FlowCellect™ Human NK Cell Characterization Kit includes three directly conjugated antibodies, Anti-CD3-APC, Anti-CD56-PE, and Anti-CD16 FITC. This three color kit is designed to characterize NK within mixed cell populations. The antibodies provided have been carefully titrated to ensure the ability to accurately measure the expression of all markers simultaneously. Sufficient reagents are provided to perform 25 three-colo…
Millipore’s FlowCellect™ DNA Damage (Histone H2A.X) and Cell Cycle Analysis Kit is designed to identify the linkage between DNA damage in relation to cell cycle checkpoints in flow cytometry applications. The kit includes a directly conjugated Anti-phospho-Histone H2A.X (Ser139) Alexa Fluor® 488 antibody plus a DNA dye (Propidium Iodide) which allows the researcher to perform bivariate analysis to identify where DNA damage ta…
Millipore’s FlowCellect™ DNA Damage (ATM) and Cell Cycle Analysis Kit is designed to identify the linkage between DNA damage in relation to cell cycle checkpoints in flow cytometry applications. The kit includes a directly conjugated Anti-phospho-ATM (Ser1981) Alexa Fluor® 488 conjugate plus a DNA dye (Propidium Iodide) which allows the researcher to perform bivariate analysis to identify where DNA damage takes place relativ…
Millipore’s FlowCellect™ Bivariate Cell Cycle Kit for DNA Replication Analysis is designed to investigate cells undergoing replication in S-phase of the cell cycle with high accuracy and confidence in flow cytometry applications. The kit includes a directly conjugated Anti-BrdU Alexa Fluor® 488 conjugate plus a DNA dye (Propidium Iodide) which allows the researcher to perform bivariate analysis to distinguish cells in two dim…
Millipore’s FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis is designed to allow the researcher to easily identify, discriminate, and quantitate cells between the G2 and M-phases of the cell cycle. The kit can be used to perform bivariate analysis to measure cell cycle distributions by discrimination of cell cycle phases without the need of sophisticated software modules or algorithms. Having the ability to distingui…
Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit is designed to enable a researcher a quick and easy way to detect the phosphorylation state of ATM, SMC1 and Histone H2A.X by flow cytometry. Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit was developed and tested using the DNA damaging reagent, Etoposide, in HeLa cells as a model system but the kit can be used with other human cell lines to determine…