Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
The Neural Stem Cell Basal Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of neural stem cells derived from rodents. When used in conjunction with bFGF or bFGF, EGF and heparin, the basal medium will allow for the proliferation of rat and mouse neural stem cells, respectively. Withdrawal of the growth factors from the Neural Stem Cell Basal Medium w…
The Mouse Neural Stem Cell Expansion Medium allows for the growth and proliferation of mouse neural stem cells. This product includes the Neural Stem Cell Basal Medium and the supplements bFGF, EGF, and Heparin. Withdrawal of the growth factors and heparin from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of mouse neural stem cells.
MilliTrace Mouse Embryonic Stem Cell Expansion Medium is a complete ready-to-use expansion medium optimized for the culture of MilliTrace GFP Reporter Mouse Embryonic Stem Cells. Puromycin Solution is provided to help maintain the expression of the green fluorescent protein (GFP) labeled transgene.
The Cardiac Stem Cell Maintenance Medium has been optimized and qualified for the growth and expansion of cardiac stem cells derived from rodent origins. Cells expanded in Cardiac Stem Cell Maintenance Medium express the correct cardiac stem cell markers and are furthermore capable of differentiating into cardiomyocytes.
ESGRO Complete™ Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF and BMP, and during routine washing steps when passaging cells.
ESGRO Complete™ Accutase™ is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete™ Clonal Grade Medium (Cat. No. SF001-500). Accutase™ does not contain mammalian or bacterial derived products.
Embryonic Stem Cell Lines derived from murine strain C57/BL6J selected for high targeting efficiency and proven germ line transmission. Cells are supplied as 2.5 x 106 ES cells per vial (approximately 2.5 population doublings per passage, shipped frozen on dry ice) and are supplied at passage 12. This ES cell line has been pre-adapted to serum-free and feeder-free cell culture conditions and is intended for use with the ESGRO…
A highly efficient method of gene trasfer into mammalian cells is through infection with retroviral vectors. The efficiency of retroviral infection is enhanced significantly, 100 to 1,000 fold in some cells, by including polybrene during the infection. Polybrene with DMSO shock is also used to mediate DNA transfer into a variety of cell types, such as: CHO, chicken embryo fibroblases, NIH3T3 cells, and myeloid cells.
Upstate's new Nitrotyrosine Assay Kit, Chemiluminescence Detection is a competitive ELISA for the quantitation of tyrosine nitration. The kit includes all reagents needed for measurement of tyrosine nitration, including white high binding 96-well plates, nitrated BSA standard, a Nitrotyrosine antibody, LumiGLO® chemiluminescent detection substrate, and wash buffers. The assay has a wide dynamic range and high precision, making…
Upstate®'s cGMP HTS Immunoassay kit is a competitive immunoassay for highly sensitive and rapid chemiluminescent quantitation of cyclic GMP (cGMP, Guanosine 3', 5'-cyclic monophosphate) from extracts, lysates or supernatants of cells of any species. The kit comprises a specific anti-cGMP antibody which recognizes all species, an alkaline phosphatase (AP)-labeled cGMP conjugate, two 96 well microplates pre-coated with ananti-Ra…
