The PureGenome™ On-Spot Tissue DNA kit allows for convenient isolation of genomic DNA in any targeted microscopic tissue area on a slide. The system combines a powerful area-specific tissue sampling technology with a one-step DNA extraction method and makes targeted tissue genomic DNA isolation easy and simple. The kit efficiently recovers genomic DNA from paraffin archives or fresh tissue sections for PCR analysis. Unlike a c…
The PureGenome™ Tissue DNA Extraction Kit is a simple procedure for the rapid isolation of total DNA (e.g. genomic, mitochondrial, parasitic, microbial, viral) from a variety of soft and solid tissues, cells and a range of biological liquids. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is also compatible with buffy coat, bone marrow, cells from culture, whole blood (fres…
A sterile, closed, disposable system for sampling fluids from sterile and aseptic processes The unique NovaSeptum sampling system is ideal for general fluid and cell culture sampling from aseptic and sterile processes. Both systems can be configured for high purity or autoclavable sampling applications and as single or multiple sampling units. A wide range of holders and connectors are also available. • Closed sampling elimin…
Containers Make Sense EMD Millipore ReCycler™ Containers guarantee solvent purity and meet the needs of the scientific community by eliminating the need to dispose of large volumes of glass bottles, styrofoam packaging inserts and corrugated cartons. Today, EMD Millipore ReCycler™ containers are available in sizes from 18.9L (5 gallon) to 1250L for the many diverse scientific applications from laboratory to small-scale manufac…
Pure recombinant high-fidelity DNA polymerase from Thermococcus kodakaraensis KOD1KOD HiFi DNA Polymerase* is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase. KOD HiFi is a high fidelity thermostable polymerase that amplifies target DNA up to 6 kbp in length with superior accuracy and yield. The enzyme’s 3′→5′ exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than any ot…
High-performance enzyme blend for long and accurate PCRKOD XL DNA Polymerase* is an optimized blend of KOD HiFi DNA Polymerase and a mutant form of KOD HiFi that is deficient in 3′→5′ exonuclease activity. This enzyme mixture is designed for reliable amplification of long, complex targets with robust yield and high accuracy. It can also be used for incorporation of dITP, dUTP and derivatized dNTPs in PCR amplicons. KOD XL DNA…
GeneJuice® Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity. This transfection reagent is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, and complex formation with DEAE-dextran. Whereas many available transfection reagents…
BugBuster® Master Mix combines BugBuster Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme™ Solution in one convenient reagent. BugBuster Master Mix allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. With the Master Mix, there is no need for dilution or separate addition steps. The two available package sizes provide sufficient reagents for protein extracti…
In H2O. A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with tissue and cell extracts, including extracts containing detergents. Each vial contains 200 mM Imidazole, 100 mM Sodium Fluoride, 115 mM Sodium Molybdate, 100 mM Sodium Orthovanadate, and 400 mM Sodium Tartrate Dihydrate. Dilute 1:100 just prior to use. Pro…
AVOID FREEZE/THAW CYCLES. The ProteoExtract Subcellular Proteome Extraction Kit is a tool for the differential extraction of proteins from adherent or suspension tissue culture cells according to their subcellular localization. The sequential extraction steps yield fractions containing cytosolic proteins, plasma membrane and organelle proteins, soluble nuclear proteins, and finally cytoskeletal and nuclear matrix proteins. To…
PhosphoSafe™ Extraction Buffer extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. The reagent, an enhancement of the CytoBuster™ Protein Extraction reagent, includes four phosphatase inhibitors. PhosphoSafe is compatible with Western blotting, immunoprecipitations, and kinase assays.
Pellet Paint® NF Co-Precipitant* is a non-fluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye™ Terminators (PE Corp.) during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with centrifugation times of 10 minutes. The easily visualized carrier provides a simple confirmation that precipitation has o…
Rapid, quantitative precipitation of DNA and RNA, including PCR clean-upPellet Paint® Co-Precipitant* is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids (1, 2). The two minute precipitation requires no low temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easil…
Overnight Express™ Instant TB Medium is a complete granulated culture medium for high-level protein production without the need to monitor cell growth in the pET and other IPTG-inducible bacterial expression systems. Cell mass and target protein yield are often increased several-fold as compared with induction using IPTG. The method is based on media components that are metabolized differentially to promote growth to high dens…
White solid. Aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3¡ä-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Intro…
Light brown lyophilized solid. Working concentration 50 mg/ml - 1 mg/ml for mammalian cell selection. Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isol…
The ProteoPlex™ 16-Well Murine Cytokine Array Kit is designed for multiplex detection and measurement of 10 important mouse cytokines in parallel from up to 15 experimental samples. The 10 analytes measured in the kit are pro- and anti- inflammatory cytokines important for the study of immune system regulation and diseases such as asthma and arthritis. Each well on the slide contains a microarray of spotted antibodies with fou…
The ProteoPlex™ 16-Well Human Cytokine Array Kit is designed for multiplex detection and measurement of 12 important human cytokines in parallel from up to 15 experimental samples. The combination of advanced surface chemistry, well-characterized capture agents, and a highly-sensitive fluorescence-based detection system provides a robust method for comparing relative cytokine abundance or cytokine quantification in serum sampl…
Novel protein markers for visible tracking & accurate sizing on any Western blotTrail Mix™ Western Markers consist of the Perfect Protein™ Western Markers supplemented with a group of three prestained indicator proteins to allow direct visualization of protein mobility during electrophoresis. Trail Mix Western Markers and Western Blot Kits have all of the features of Perfect Protein Western Markers and Western Blot Kits, plus…
The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of Novagen's expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10 to 8–1 × 10–9 M) enables sensitive, specific detection of His•Tag fusion prote…
Benzonase Nuclease - Effective viscosity reduction and removal of nucleic acids from protein solutions Benzonase Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. Benzonase Nuclease is effective over a wide range of conditions and possesses an exceptionally high specif…
Purification of His•Tag fusion proteins by metal chelation chromatographyThe His•Bind® family of products offers a wide selection of supports designed for rapid one-step purification of proteins containing the His•Tag® sequence by immobilized metal affinity chromatography (IMAC). The His•Tag sequence (6, 8 or 10 consecutive histidine residues) binds to divalent cations (Ni2+) immobilized on NTA*- and IDA-based His•Bind resins.…
The most common method for transformation of plasmids into E. coli is to use chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility and convenience are achieved using prepared competent cells. Novagen offers the widest selection of competent cells available for protein expression. Our strains offer a variety of options that enable you to maximize the yield an…
Pseudoproline dipeptides are undoubtedly the most powerful tools described to date for enhancing synthetic efficiency in Fmoc SPPS. Novabiochem® now offers 28 pseudoproline dipeptides - representing the widest selection on the market today The routine use of pseudoproline dipeptides has been found to: Avoid costly and unnecessary repeat syntheses of failed sequences Increase purity of crude products Simplify HPLC puri…
