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The Mammalian Cell Transfection Kit is an optimized system suitable for the introduction of DNA into mammalian cells. DNA is introduced to the cells as a calcium phosphate precipitate. Transfected cells integrate and express the exogenous DNA at a higher efficiency, producing stable clones of the specifically altered genotype and phenotype. This system has been used to introduce various plasmids into a broad range of cell line…

Enzyme-Free Cell Dissociation Solution is a Hank's based formulation of chelating agents and agents to stabilize their activity on cells. This reagent does NOT contain porcine trypsin or any other protein. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cyto…

Enzyme-Free Cell Dissociation Solution is a PBS based formulation of chelating agents and agents to stabilize their activity on cells. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cytotoxic effects sometimes associated with 0.5 mM EDTA. It can be used to…

ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell VM was derived from the ventral mesencephalon region of human fetal brain. Immortalized by retroviral transduction with the v-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell VM retain…

EndoGRO Human Umbilical Vein Endothelial Cells (HUVEC) are a commonly studied human cell type extracted from human neonatal umbilical cords. They are relatively easy to culture, and provide a valuable cell model for many vascular biology research applications, including inflammation, angiogenesis, atherosclerosis, blood clotting, vasoconstriction, and vasodilation. EndoGRO™ HUVEC are provided at passage 1, and have been qualit…

ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture and expansion of ENStem-A Human Neural Progenitor Cells. When used in conjunction with L-Glutamine (not provided) and FGF-2 (provided), the expansion medium will allow for the maintenance and proliferation of ENStem-ATM Human Neural Progenitor Cells.

ReNcell NSC Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will…

ReNcell Neural Stem Cell Freezing Medium is qualified for use with ReNcell immortalized human neural progenitor cell lines, CX and VM cultured in serum-free conditions with ReNcell NSC Maintenance Medium. The optimized formulation allows for consistent cryopreservation and high viability upon thawing and plating.

ENStem-A™ Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A™ human neural progenitor cells to a neuronal lineage. The medium has been extensively validated on ENStem-A™ Human Neural Progenitor Cells. Using ENStem-A™ Neuronal Differentiation Medium, relatively pure populations of neuronal cells (90% bIII-tubulin positive) are obtained. Very low levels of…