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Human CRISPR Poison Exon Knockout Library
The human, paired guide Poison (pgPoison) library targets 3` splice sites with paired guide RNAs for alternative exon removal (pgRNAs), induce skipping of "poison" cassette exons and corresponding upstream constitutive exons in ultraconserved regions.
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This compact library (targets 963 exons) allows direct comparison between loss of a constitutive coding exon (knockout) with loss of a poison exon.
