Promega Corp. Products & Reviews

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase.   The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary scre…

The Maxwell® 16 LEV simplyRNA Kits are for use with the Maxwell® 16 Instrument configured with the LEV High Strength Magnetic Rod and Plunger Bar Adaptor.   This RNA purification procedure is a simple method with minimal lysate handling before automated purification on the Maxwell® 16 Instrument. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such…

The mass spectrometry-compatible yeast and human protein extracts are designed specifically for mass spectrometry applications.   The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spectrometry (LC/MS) analysis. Both the yeast and human extracts also are available in an intact undigested form to provide a test material for optimizing mass spec protei…

Switch Seamlessly from Your Current Dual-Luciferase® Assay!

The GloMax® Explorer System is the newest member of the GloMax® System detection instruments. This multimode reader allows you to get up and running quickly, generating the data you need from your experiments. Simply unpack it, plug it in, and begin your experiments.

NanoLuc® Binary Technology (NanoBiT) is a two-subunit system based on NanoLuc® luciferase that can be applied to the intracellular detection of protein:protein interactions (PPIs) in live cells. The NanoBiT® system is composed of two small subunits, Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acid peptide), that are expressed as fusions to target proteins of interest. The LgBiT and SmBiT subunits have been independ…

The CellTiter-Glo® Luminescent Cell Viability Assay(a,b) is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure in…

The CellTiter-Glo® 2.0 Assay provides a homogeneous method for determining the number of viable cells in culture by measuring the amount of ATP present, which indicates the presence of metabolically active cells. The CellTiter-Glo® 2.0 Assay is based on the original CellTiter-Glo® Assay chemistry but with improved storage convenience for easy implementation. The CellTiter-Glo® 2.0 Assay is provided as a single, ready-to-use re…

To shorten the time required to prepare samples for LC-MS/MS analysis, we have developed an optimized protocol in which rapid and efficient trypsin digestion is achieved at temperatures as high as 70°C. Using this approach, proteolytis reaction times are dramatically shortened, no chemical denaturants are added and off-line sample cleanup is unnecessary, leading to shorter preparation times and diminished sample losses.

The Lactate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of L-lactate in biological samples. Lactate is produced by glycolysis, a major metabolic pathway responsible for glucose homeostasis and energy production (1,2). Once considered merely a byproduct of glycolysis, lactate is now considered an important regulatory molecule of intermediate metabolism involved in cancer development, diabet…

Automated RNA Extraction from FFPE Tissue Sections

Automated RNA Purification from Cells using a Low Elution Volume

Automated Nucleic Acid Purification with Integrated Quantitation

Easy-to-Use, Dedicated Microplate Developed and optimized with our cell and gene reporter assays

Ultrasensitive Single Tube Luminometer Two fluorescence modules available

Process swabs and storage card punches for direct amplification.

An affordable benchtop instrument for Sanger sequencing and fragment analysis.

Ethanol-Free Purification for High-Quality gDNA Every Time

High-throughput extraction of gDNA from a range of sample types in 24-or 96-well format

High-throughput, high-purity gDNA from blood

A Fluorometric Method for Estimating the Number of Nonviable Cells Present in Multiwell Plates

Extracts RNA from Tissues, Cultured Cells and White Blood Cells

Measure Kinase-Ligand Affinity in Live Cells The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay measures compound binding at select target kinases in intact cells. The assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET™ TE Assay measures the apparent affinity of test compounds by competitive displacement of the NanoBRET™ tra…

Characterize MSI Status of Your Research Samples

A Complete Solution for Monitoring LC and MS Instrument Quality Control and Performance. In combination with an accompanying software tool, the 6 × 5 LC-MS/MS Peptide Reference Mix provides a convenient way to assess LC column performance and MS instrument parameters, including sensitivity and dynamic range. Consists of 30 peptides; 6 sets of 5 isotopologues of the same peptide sequence, differing only in the number o…