For standard cloning experiments competent cells are available in two formats: high efficiency at greater than 108cfu/μg for cloning (JM109, HB101), and subcloning efficiency at greater than 107cfu/μg (HB101). JM109 cells have the additional feature of blue/white screening for recombinants. For protein expression, JM109(DE3), BL21(DE3) or KRX competent cells can be used. The Single Step (KRX) Competent Cells are designed fo…
Diamond™ Nucleic Acid Dye is a safe, sensitive fluorescent dye that binds to single-stranded DNA, double-stranded DNA and RNA, and can be used to stain and visualize nucleic acids in gels. Diamond™ Nucleic Acid Dye is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system. Significantly less mutagenic than traditional ethidium bromide, as proven by Ames…
The BenchTop DNA Markers, supplied in a stabilizing solution of 1X Blue/Orange Loading Dye, come in a variety of different sizes to meet your DNA electrophoresis needs.
Promega has partnered with ATCC to offer unparalleled STR testing service for human cell line authentication. This service provides easy access to proven and comprehensive validation techniques. Promega's proprietary PowerPlex® and GenePrint® STR technology coupled with ATCC's considerable experience profiling human cell lines and extensive internal assets around STR profiling data interpretation is particularly r…
High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling. Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dNTPs Features & Benefits: Dependable - PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzyme…
GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polyemrase in a specially formulated mixture with a proprietary thermostable proofreading polyemrase. This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches and a highly processive polyemrase allows the polyemras…
QuantiFluor® ONE system maximizes sensitivity and efficiency for dsDNA quantitation. The QuantiFluor® ONE dsDNA System contains a fluorescent double-stranded DNA-binding dye (504nmEx/531nmEm) developed for use in an “add-and-read” format for dye and standard, making sample quantitation easy. This system enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA). The QuantiFluor® ONE dsDNA Sy…
The Maxwell® Clinical Sample Concentrator (CSC) automated nucleic acid extraction system is designed for clinical laboratories needing to process multiple samples for critical downstream assays. This easy-to-use, cGMP-compliant system provides consistent, high-quality results with every run, allowing you to manage your workflow and gain efficiency. The purification methods use sample lysis and binding to paramagnetic par…
Designed for use with the Maxwell Clinical Sample Concentrator and the Maxwell CSC blood purification method as an IVD medical device for automated isolation of human genomic DNA from whole blood. Because the Maxwell® CSC Blood DNA Kit is manufactured to such strict specifications, you can count on consistent, high quality DNA suitable for use in downstream amplification assays. Furthermore the intuitive interface…
The Maxwell® CSC DNA FFPE Kit is used in combination with the Maxwell® CSC Instrument to provide an easy method for efficient, automated purification of genomic DNA (gDNA) from human FFPE breast, lung or colon tissue samples. The Maxwell® CSC DNA FFPE (formalin fixed, paraffin embedded) kit is intended for use, in combination with the Maxwell® CSC Instrument and the Maxwell® CSC DNA FFPE purification method, as an…
The QuantiFluor® ONE dsDNA Dye is a fluorescent double-stranded DNA-binding dye (504nmEx/531nmEm) prepared for an “add-and-read” format making sample quantitation easy. This dye enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA). The QuantiFluor® ONE dsDNA Dye is intended for use as a general purpose reagent in the US and is suitable for use by clinical laboratories as a component of la…
The Maxwell® 16 LEV RNA FFPE Purification Kit is designed to extract RNA rapidly from FFPE samples using the Maxwell® 16 Instrument (Cat.# AS2000 and AS3000). The FFPE kit is specifically designed for optimal purification of RNA from 5μm thin sections of formalin-fixed, paraffin-embedded (FFPE) tissue samples. The Maxwell® 16 LEV RNA FFPE Purification Kit is optimized to yield a final RNA concentration that maximi…
The Glucose-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glucose in biological samples.
The Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamate in biological samples.
The Glutamine/Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples.
The Succinate-Glo™ JmjC Demethylase/Hydroxylase Assay is a bioluminescent assay for detecting the activity of JumonjiC (JmjC) histone demethylases and many Fe(II)/α-ketoglutarate-dependent dioxygenases that use α-ketoglutarate as substrate and release succinate as a product.
Count on minimal interference from test compounds when screening your compound library using luminescent, ATP-based kinase assays. We offer the ADP-Glo™ Kinase Assay, Kinase Selectivity Profiling Systems and hundreds of Kinase Enzyme Systems. These sensitive, reliable assays are easily scaled to meet your throughput needs and can be used with virtually any kinase and kinase substrate combination.
IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It is an engineered recombinant protease overexpressed in E. coli that cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments. The protocol for a standard reaction is to add the IdeS Protease to the IgG sample, add 1 unit of IdeS Protease per 1µg of IgG to be digested…
IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus. It is an engineered recombinant protease overexpressed in E. coli. Like IdeS Protease, IdeZ Protease specifically cleaves IgG molecules below the hinge region to yield F(ab′)2 and Fc fragments. However, IdeZ Protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS Protease.
Automated Purification of High-Quality Total RNA with an Enhanced miRNA Enrichment
Preformulated PCR Master Mix Components to Determine Optimal PCR Amplification Conditions
Standardized Screening Panel Amplify key functional regions associated w/Azoospermia Factor (AZF)
Automated ccfDNA Purification with a Simple Three-Step Protocol
