Thermo Fisher Scientific Products & Reviews

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I. Highlights • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides) • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers A…

Thermo ScientificT4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity. Highlights • Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment (see Reference1) • Active in Therm…

Thermo Scientific T4 Polynucleotide Kinase (PNK) is a tetrameric protein composed of four identical subunits. It is widely used in molecular biology to radiolabel nucleic acids and to add a phosphate to the 5'-end of dephosphorylated nucleic acids to facilitate ligations. Features • Phosphorylates 5’ ends • Transfers 32P to DNA and RNA

Thermo Scientific Exonuclease I digests single-stranded DNA 3' to 5' (requires Mg2+ and a free 3'-OH). The nuclease is inactivated by heating at 80°C for 15 minutes.

Thermo Scientific Shrimp Nuclease is an endonuclease that cleaves phosphodiester linkages in DNA to yield di- and oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. The enzyme has a high preference for double-stranded DNA, and can be used to selectively degrade dsDNA, leaving ssDNA and RNA intact. The enzyme is inactivated after 15 minutes at 65 °C.

Thermo Scientific RNase A (ribonuclease A) is supplied as a chromatographically pure freeze-dried powder, salt-free for easy reconstitution in most buffers.

Thermo Scientific Ribonuclease H (RNase H) is an endonuclease that degrades the RNA portion of DNA-RNA hybrids. It hydrolyses phosphodiester bonds of RNA in RNA:DNA heteroduplexes. It produces products with 3’-OH and 5’-phosphate. RNase H will not degrade single or double-stranded DNA or RNA.

Thermo Scientific Shrimp Alkaline Phosphatase (SAP) catalyzes the dephosphorylation of 5´ phosphates from DNA and RNA and is completely and irreversibly inactivated by heating at 65°C for 15 minutes. SAP is active on 5´ overhangs, 5´ recessed and blunt ends.

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indoyl-ß-D-galactopyranoside) is a chromogenic substrate used to identify recombinant plasmids. In the presence of X-Gal and IPTG, lac+ bacterial colonies appear blue, whereas recombinant colonies appear white.

The Thermo Scientific 96-Well Semi-Skirted Plates, Fast Block hold a maximum of 0.1 mL of well volume when used in conjunction with adhesive and heat seals. The plates are directly compatible with all Applied Biosystems thermal cycler fast blocks, no adapters necessary. These semi-skirted low profile thin walled plates feature a raised deck to aid in spill prevention and come with a cut corner at the A1 location. The 96-Well S…

Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications. Random Decamers are truly random oligo deoxyribonucleotides that can be used to create a pool of cDNAs for subsequent applications, thus providing maximum flexibility.

Thermo Scientific Random Hexamers are truly random oligo deoxyribonucleotides. Hexamers are ideal primers for mRNA with or without a poly-A tail. Hexamers bind more often than decamers, giving higher yields and increased sensitivity when amplifying short fragments.

Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications. Magnesium ions (Mg2+) are an important component of PCR reactions where they interact with the DNA template, dNTPs, and Taq DNA Polymerase.

Adhesive film and foils seals for PCR and qPCR plates. Gas permeable seals and crystallography seals. Adhesive PCR Film Seal:Thermo Scientific Adhesive PCR Plate Seals are a reliable method of sealing plates for PCR applications. The high integrity sealing materials give the best protection against evaporation for high efficiency PCR. Highlights • Clear for sample inspection • Strong adhesive prevents evaporation during therm…

Power SYBR® Green RNA-to-CT 2-Step Kit is a flexible kit that enables a two-step protocol for performing reverse transcription and cDNA quantitation by real-time PCR. The kit includes the High Capacity RNA-to-cDNA and the PowerSYBR® Green PCR Master Mix. The High Capacity RNA-to-cDNA Kit is used to first reverse transcribe RNA into cDNA and the resulting cDNA is then quantitated using the PowerSYBR® Green PCR Master Mix for…

Ambion Anti-miR™ miRNA Inhibitors are chemically-modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous miRNA molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs, and enable detailed study of miRNA biological effects.

Ambion Pre-miR™ miRNA Precursor Molecules are small, chemically-modified double-stranded RNA molecules designed to mimic endogenous mature miRNA molecules. These ready-to-use miRNA mimics, which are similar to but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters identical to those used for siRNAs and enable detailed study of miRNA biological effects via gain-of-function e…

Silencer Pre-Designed siRNAs are Ambion-designed siRNAs available for all human, mouse, and rat gene targets in the RefSeq database. These siRNAs are designed for maximum potency and specificity using a highly effective and extensively tested algorithm. Each siRNA is synthesized to the highest quality standards and is provided with full sequence information. Silencer Validated siRNAs are Ambion-designed siRNAs that have been…

Ambion Silencer Select Pre-Designed and Validated siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNA with unrivaled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Ambion Silencer® In Vivo Ready siRNAs undergo the additional purification and testing necessary for introduction of siRNA into animals. After HPLC purification and annealing, each siRNA is dialyzed to remove salt, sterile filtered, and endotoxin tested. All Silencer Pre-Designed and Validated siRNAs are available in this format, as are Custom Synthesized siRNAs.

Abmion Validated GAPDH Positive Control siRNA and non-targeting Negative Control siRNAs are available for human, mouse and rat with the same chemical modifications for enhanced efficacy as in other Ambion Silencer Select siRNAs.

For the first time, you can obtain the benefits of Ambion Silencer Select siRNAs in a convenient 96- or 384-well plate format, plated to your specifications. These siRNA collections enable medium- and high-throughput analysis of phenotypes resulting from inhibition of sets of targets from specific functional classes or your favorite list of targets.

Silencer siRNA Libraries are sets of Ambion-designed siRNAs targeting all of the genes in a genome or a particular gene class, making them ideal for pathway analysis and target identification/validation. Most libraries include three individual siRNAs per gene for enhanced data reliability. Full sequence information is provided with each library.

Applied Biosystems currently offers five TaqMan Copy Number Assays to detect copy number variation. Copy number is an important polymorphism in the human genome associated with genetic diseases such as cancer, immune diseases, and neurological disorders. TaqMan Copy Number Assays were designed to detect CYP2D6, CYP2A6, CYP2E1, GSTT1, and GSTM1.

The TaqMan® Custom Array is a 384-well micro fluidic card that enables a researcher to perform 384 simultaneous real-time PCR reactions without the need to use liquid-handling robots or multichannel pipettors to load. This low- to medium- throughput array allows for 1-8 samples to be run in parallel against 12-384 TaqMan® Gene Expression Assay targets that are pre-loaded into each of the wells on the array. The TaqMan Custom…