Drug discovery > Basic Research Products & Reviews

High-Throughput Extraction of DNA from FFPE Samples Robust, precipitation-free protocol, no chance of lost pellets High yields of pure DNA from FFPE samples without using xylene or other hazardous chemicals Ideal for use in downstream applications including qPCR and next-generation sequencing (NGS)

The Wizard MagneSil Tfx™ System provides a simple, rapid method for isolating transfection-quality plasmid DNA in a 96-well, high-throughput format. The use of paramagnetic particles for lysate clearing as well as DNA capture circumvents the need for centrifugation or vacuum manifolds, allowing DNA purification to be completely automated.

The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:…

The Wizard ® SV 96 Genomic DNA Purification System provides a high-throughput, membrane-based technique for consistent preparation of genomic DNA from cultured cells and tissue, including mouse tails. Amplifiable genomic DNA can be isolated from up to 5 × 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step.

Column-based method for rapid concentration and cleanup of DNA.

Column-based method for rapid concentration and cleanup of RNA.

Isolate High-Molecular-Weight DNA for Long-Read-Sequencing applications.

LDH-Glo™ Cytotoxicity Assay detects cell death from a small number of cells, including 3D cell cultures, based on the release of lactate dehydrogenase.

The RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay provides a no-wash, one-step Annexin V assay that allows continual monitoring of cell state to determine apoptotic onset using a plate-reading luminometer.

The GSH/GSSG-Glo™ Assay is a bioluminescent assay to detect and quantitate total glutathione ratios.

The Caspase-Glo ® 1 Inflammasome Assay  directly measures inflammasome activation with a simple add-mix-measure protocol.

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism of action of antibodies through which virus-infected or other diseased cells are targeted for destruntion by components of the cell-mediated.

The TNT® Coupled Reticulocyte Lysate Systems offer researchers an alternative for eukaryotic cell-free protein expression: a single-tube, coupled transcription/translation system. The TNT® Lysate Systems greatly simplify the process and reduce the time required to obtain in vitro translation results.

Wizard® Plus Megapreps DNA Purification System provides a simple and rapid method for large-scale purifications of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. The system yields greater than one milligram of high-copy-number plasmid DNA (200–20,000bp) from a 1,000ml culture in less than th…

ProteaseMax™ Surfactant is a high-quality reagent for use in protein and peptide sample preparation.   ProteaseMax™ Surfactant solubilizes proteins and ensures fast and improved protein digestion with trypsin or chymotrypsin. It is designed to degrade over the course of a digestion reaction, yielding products that are compatible with downstream methods such as mass spectrometry (MS) and liquid chromatography (LC). For…

Predesigned probe assays for gene expression analysis

Custom adapters for multiplex next-generation sequencing

Predesigned primers for gene expression analysis

Specialized purification to ensure high performance

Larger quantities for in vivo, high throughput and commercial projects  

For Mpox qPCR assay development

If you commercialize Molecular Diagnostics (MDx), create Laboratory Developed Tests (LDTs), or conduct preclinical research, then your final kit, assay, or modality must have an extraordinary level of quality to comply with strict regulatory or study standards. In the case of oligonucleotide raw materials available with custom specifications, the only option you should consider is Custom cGMP Oligos.

Modulation of gene expression with modified oligos

Proteinase K from Tritirachium album ELEVATE  

Proteinase K from Tritirachium album ELEVATE