Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
Corning® T-Cell Culture Supplement with ConA (IL-2 culture supplement), rat, 100 mL, used to promote proliferation and activation of T-cells and support high-titer HIV production by leukocytes. 1/pack, formerly Cat. #40115StorageShelf life: >= 3 months from date of shipment at 2-8°C
Corning® Bovine Pituitary Extract (BPE), 15mg, is a broadly used supplement to culture a variety of epithelial and endothelial cells. BPE contains growth factors (e.g., basic FGF) and hormones. 1/packSourceBovine pituitary glandsQuality Tested for ability to promote proliferation of Fetal Bovine Heart Endothelial Cells (FBHEC) in medium containing 10% newborn calf serum Filtered (0.2 µm membrane) and tested and found negativ…
This is suggested replacement for 354141.Corning® BioCoat™ Angiogenesis System for Endothelial Cell Invasion comprises a 3.0 µm Corning FluoroBlok™ PET Membrane 24-well Permeable Support System pre-coated with Corning® Matrigel®. Packaged ready to use with Falcon® Companion Plate, 1/case Allows rapid data collection Eliminates need for manual cell scraping and counting Increases throughput and productivityShelf life: >= 3 m…
This is suggested replacement for 354143.HTS insert plates are arrays of individual cell culture inserts connected by a rigid, robotics-friendly holder. This single-unit design makes insert plates ideal for running automated, high throughput drug transport (Caco-2 cells) cell toxicity studies or cell migration and invasion studies.• Predictive in vitro model for screening pro- and anti-angiogenic compounds Optimized human f…
Highly reproducible assay system Ideal for screening anti-angiogenic agents Rapid image acquisition and data quantification
Highly reproducible assay system Ideal for screening anti-angiogenic agents Rapid image acquisition and data quantification
Corning® Poly-D-Lysine, 20mg, is used to culture a wide variety of cell types, particularly neurons, glial cells, and transfected cells.SourceSyntheticQualityShown to promote cell attachment and spreading of rat cerebellar granule cellsFound negative for bacteria, fungi, and mycoplasmaMolecular Weight500-550 kDPreparation and StorageStable for three months at 2° to 8°C (lyophilized) or three months at -20°C (solubilizedHandlin…
The fluorescent dye calcein acetoxymethylester (calcein AM) can be used with Corning FluoroBlok Cell Permeable Supports and Insert Systems to label cells when performing analyses such as tumor cell invasion, endothelial cell migration, endothelial cell tubulogenesis, and other cell-based assays.The cell viability indicator calcein AM is a non-fluorescent, cell permeant compound that is hydrolyzed by intracellular esterases int…
The fluorescent dye calcein acetoxymethylester (calcein AM) can be used with Corning FluoroBlok Cell Permeable Supports and Insert Systems to label cells when performing analyses such as tumor cell invasion, endothelial cell migration, endothelial cell tubulogenesis, and other cell-based assays.The cell viability indicator calcein AM is a non-fluorescent, cell permeant compound that is hydrolyzed by intracellular esterases int…
The fluorescent dyeDiIC12(3) can be used with Corning FluoroBlok Cell Permeable Supports and Insert Systems to label cells when performing analyses such as tumor cell invasion, endothelial cell migration, endothelial cell tubulogenesis, and other cell-based assays.DiIC12(3) is a lipophilic neuronal tracer that is commonly used for labeling neuronal projections as well as lipid bilayers in other cell types. Because DiIC12(3) ex…
Corning® rLaminin-521, Human, 20μg Vial Corning rLaminin-521 (Human) is a heterotrimer composed of α5, β2, and γ1 chains expressed in a mammalian cell culture system. rLaminin-521 (Human) supports long-term self-renewal of human pluripotent stem cells (hPSC), including embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) in defined and xeno-free environments. rLaminin-521 provides additional benefits, includin…
Corning® rLaminin-521, Human, 100μg Vial Corning rLaminin-521 (Human) is a heterotrimer composed of α5, β2, and γ1 chains expressed in a mammalian cell culture system. rLaminin-521 (Human) supports long-term self-renewal of human pluripotent stem cells (hPSC), including embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) in defined and xeno-free environments. rLaminin-521 provides additional benefits, includi…
Corning® Collagen I, bovine, 30mg, is used either as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function. (Same as Cat. #40231)SourceBovine dermisQualityPurity > 95% by SDS-PAGEShown to promote attachment and spreading of HT-1080 human fibrosarcoma cellsTested for formation of a firm gel by exposure to ammonia vapors fr…
Corning® Laminin, mouse, 1mg, is used as a thin coating on tissue-culture surfaces or as a soluble additive to culture medium. Laminin is a major component of basement membranes. It has numerous biological activities including promotion of cell adhesion, migration, chemotaxis, growth, and differentiation, including neurite outgrowth.QualityPurity >90% by SDS-PAGE (contains entactin)Tested for ability to initiate neurite outgro…
Corning® Collagen IV, mouse, 1mg, is used as a thin coating on tissue-culture surfaces to promote cell attachment and proliferation and to study the collagen's effects on cell behavior.SourceSource material tested for HBsAG, for anti-HIV-1 and anti-HIV-2, and anti-HCV virus-1 and virus-2.QualityElectrophoretically homogeneous (SDS-PAGE)Tested and found negative for bacteria, fungi, and mycoplasmaPreparation and StorageStable f…
Corning® Collagen I, rat tail, 100mg, is used either as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function.SourceRat Tail TendonQualityPurity = 90% by SDS-PAGETested for: ability to promote attachment and spreading of HT-1080 human fibrosarcoma cellsForms firm gel up to a dilution of 1:10; further dilution will decreas…
Corning® Ultrapure Laminin, mouse, 1mg, is entactic free and is used as a thin coating on tissue-culture surfaces or as a soluble additive to culture medium. Laminin is a major component of basement membranes. It has numerous biological activities including promotion of cell adhesion, migration, chemotaxis, growth, and differentiation, including neurite outgrowth.QualityPurity > 95% by SDS-PAGETested for ability to initiate ne…
Corning® Collagen I, human, 0.25mg, is used either as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function. (Same as Cat. #40243)SourceHuman PlacentaQualityElectrophoretically homogeneous (SDS-PAGE)Shown to promote attachment and spreading of HT-1080 human fibrosarcoma cellsTested and found negative for bacteria, fungi,…
Corning® Collagen IV, human, 0.25mg, is used as a thin coating on tissue-culture surfaces to promote cell attachment and proliferation and to study the collagen's effects on cell behavior.SourceHuman placenta. NOTE: Source material tested for HBsAG, for anti-HIV-1 and anti-HIV-2, and anti-HCV virus-1 and virus-2.QualityElectrophoretically homogeneous (SDS-PAGE)Tested and found negative for bacteria, fungi, and mycoplasmaPrepar…
Corning® Cell Recovery Solution, 100 mL, enables the recovery of cells cultured on Corning® Matrigel® Basement Membrane Matrix for subsequent biochemical analyses.QualityTested for ability to depolymerize a 1mm thick layer of gelled Matrigel Matrix after one hour at 2° to 8°CFiltered (0.2 µm membrane) and tested for bacteria, fungi, and mycoplasmaPreparation and StorageStable for at least three months at 2° to 8°C.HandlingCorn…
Laminin is a major component of basement membranes. It has numerous biological activities including promotion of cell adhesion, migration, chemotaxis, growth, and differentiation, including neurite outgrowth.Molecular Weight900 kD (unreduced)SourceEngelbreth-Holm-Swarm (EHS) mouse tumorQualityPurity >/ 90% by SDS-PAGETested for ability to initiate neurite outgrowth of NG-108 Mouse neuroblastoma cellsFound negative for the pres…
Corning® Collagen I, human, 10mg, is effective as a gel or thin coating at a recommended concentration of 0.2-2µg/cm2 of growth surface depending on cell type (guidelines for coating included with product).FormulationFrozen in 2mM HClSourceHuman placenta. Source material tested for hepatitis B antigen and HIV-1 antibody.QualityElectrophoretically homogeneous (SDS-PAGE)Shown to promote attachment and spreading of HT-1080 human…
Corning® ITS Premix Universal Culture Supplement, 5 mL, contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media. The concentrated supplement stimulates cell proliferation of a variety of cells under serum-reduced conditions. 5 mL (5L equivalent), 1/packThe ITS Premix (354350 and 354231) contains the following when used at the recommended dilution: Insulin…
Corning® ITS + Premix Universal Culture Supplement, 20 mL, contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media. The concentrated supplement stimulates cell proliferation of a variety of cells under serum-reduced conditions. 20 mL (2L equivalent), 1/packFormulationCorning ITS+ Premix: As an aqueous solution containing human recombinant insulin, human t…
Corning® BioCoat™ Collagen I 6-well Clear Multiwell Plate is comprised of clear TC-treated polystyrene with a uniform application of rat tail collagen type I. Flat bottom, 5/case
