Drug discovery > Basic Research Products & Reviews

The Click-iT Plus EdU Alexa Fluor 350 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed cova…

The Click-iT Plus EdU Alexa Fluor 594 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed cova…

CellTrace CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace CFSE dye. Using this dye, eight or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of dist…

CellTrace Violet Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Violet dye. Using this dye, ten or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of di…

CellTrace Far Red Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Far Red dye. Using this dye, six or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of…

CellTrace Yellow Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Yellow dye. Using this dye, six or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of di…

CellTrace Blue Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Blue dye. Using this dye, five generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of distinct gene…

Hoechst 33342 Ready Flow Reagent is a bright, easy-to-use cell-permeable stain for cell cycle analysis on a flow cytometer. When bound to double-stranded DNA, the dye emits blue fluorescence when excited with UV light, with an emission maximum at 461 nm.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteCell-permeable and effective in fixed and live-cell staining for cell cycl…

FxCycle Violet Ready Flow Reagent is a bright, ready-to-use dsDNA stain for cell cycle analysis in fixed cells using flow cytometry. The cell-impermeable dye binds ds DNA after cells are fixed, eliminating RNase treatment. It is excitable by both UV and 405 nm lasers with an emission peak at 461 nm. FxCycle Violet Ready Flow Reagent is an ideal choice for DNA content analysis and can be included in multi-color cell-cycle studi…

The AbC Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry. It addresses issues associated with cellular-based compensation controls such as poorly expressed antigens and dimly stained cells.FeaturesSpecies reactivity—for use with all isotypes of…

The AbC Total Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse, rat, hamster, or rabbit antibodies with any laser in flow cytometry. It addresses issues associated with cellular-based compensation controls such as poorly expressed antigens and dimly stained cells.FeaturesBroadest multispecies react…

ArC Amine Reactive Compensation Bead Kit is a ready-to-use, bead-based compensation tool specifically optimized for use with LIVE/DEAD™ Fixable dead cell stain kits. The kit contains two components: the ArC reactive beads (Component A) bind any of the amine-reactive dyes and provide a positive signal; the ArC negative beads (Component B) have no reactivity and provide a negative compensation control. Results are analyzed by fl…

CountBright™ Absolute Counting Beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range from UV to 635 nm excitation wavelengths and 385-800 nm emission wavelengths. CountBright™ Absolute Counting Beads can be used to compare bead events to cell events using flow cytometry, allowing the absolute number of cells in a sample to be calculated. Cell counts are more accurate and less compl…

The Flow Cytometry Size Calibration Kit uses nonfluorescent particle-size calibration standards for a simple, accurate way to determine cell sizes by flow cytometry. The kit contains six suspensions of highly uniform polystyrene microspheres with different diameters: 1.0 µm, 2.0 µm, 4.0 µm, 6.0 µm, 10.0 µm and 15.0 µm. All of the microsphere suspensions are provided in convenient dropper vials.

The LIVE/DEAD BacLight Bacterial Viability and Counting Kit allows researchers to reliably distinguish and quantitate live and dead bacteria with the aid of a flow cytometer, even in a mixed population containing a range of bacterial types. This kit uses a mixture of two nucleic acid stains - green-fluorescent SYTO™ 9 dye and red-fluorescent propidium iodide - for viability determinations, and a calibrated suspension of micros…

The LIVE/DEAD FungaLight Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO™ 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. SYTO 9 stain generally labels all yeast in a population, with or without intact membranes. Propidium iodide penetrates only…

The LIVE/DEAD BacLight Bacterial Viability Kit for microscopy and quantitative assays provides a convenient and easy-to-use solution for monitoring the viability of bacterial populations based on the membrane integrity of the cell. Cells with a compromised membrane that are considered to be dead or dying will stain red, whereas cells with an intact membrane will stain green.

The pHrodo™ Green E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry enables the detection of phagocytic activity in whole blood samples and live cell lines using flow cytometry. With no need for quenching and wash steps, the kit saves time and minimizes uncertainty over particle signals from internalized particles.The pHrodo™ Green E. coli BioParticles™ assay provides sensitive detection without the need for quenching…

The pHrodo Green S. aureus BioParticles™ Phagocytosis Kit for Flow Cytometry enables the detection of phagocytic activity in whole blood samples and live cell lines using flow cytometry. With no need for quenching and wash steps, the kit saves time and minimizes uncertainty over particle signals from internalized particles.The pHrodo Green S. aureus BioParticles assay provides sensitive detection without the need for quenching…

The LIVE/DEAD Fixable Blue Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Blue Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells wit…

The LIVE/DEAD Fixable Violet Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Violet Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells…

The LIVE/DEAD Fixable Aqua Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Aqua Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells wit…

The LIVE/DEAD Fixable Yellow Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Yellow Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells…

The LIVE/DEAD Fixable Green Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Green Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells w…

The LIVE/DEAD Fixable Red Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Red Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells with…