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Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.

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iDeal ChIP-seq kit for Transcription Factors

Diagenode

The iDeal ChIP Kit for Transcription Factors. Diagenode’s iDeal ChIP-seq Kit for Transcriptions Factors is a highly specialized solution for robust ChIP-seq results transcription factors. Unlike competing solutions, our kit utilizes a highly optimized protocol and is backed by validation with a broad number and range of transcription factors. The kit provides high yields with excellent specificity and sensitivity.   Adv…

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iDeal ChIP-seq Kit for Histones

Diagenode

Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit for Histones has everything you need for a successful start-to-finish ChIP of histones prior to Next-Generation Sequencing. The complete kit contains all buffers and reagents for cell lysis, chromatin shearing, immunoprecipitation and DNA purification. In addition, unlike competing solutions, the kit contains positive and ne…

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Tag Kit for ChIPmentation

Diagenode

Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fragments in one step. In our ChIPmentation technology we combine chromatin immunoprecipitation and tagmentation in one streamlined workflow where the tagmentation step occurs directly on chromatin. The TAG Kit for ChIPmentation has been developed for researchers who would like to perform Ch…

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Cas9 Nuclease Protein

Diagenode

Cas9 Nuclease protein NLS, in complex with guide RNA, offers benefits for CRISPR/Cas9 technology over plasmid or mRNA transfection: Off-target mutations rarely occur Prolonged expression of Cas9 increases off-target editing but RNP delivery is naturally transient Transfection/injection is easy Cas9-NLS bypasses transcription and translation by delivering the protein directly The efficiency of the guide RNA in vit…

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MagMeDIP qPCR Kit

Diagenode

Perform MeDIP (Methylated DNA Immunoprecipitation) followed by qPCR to estimate DNA methylation status of your sample using 5-methylcytosine antibody. Our kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol. Characteristics Generate highly consistent results with internal controls in 24h Minimize error with many reagents in 1 tube Optimized pu…

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MagMeDIP seq

Diagenode

Perform MeDIP (Methylated DNA Immunoprecipitation) followed by sequencing to get DNA methylation status of your sample using 5-methylcytosine antibody and magnetic beads. Our kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol. This package contains everything you need from gDNA extraction to library preparation and quality check. It allows you to perfo…

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scATAC-seq service

Diagenode

Assessing the genome-wide chromatin accessibility from individual cells provides unique insights of gene expression regulation for a number of fields such as development, oncology, and neurology. Bio-Rad’s SureCell ATAC-seq provides a fast, robust, and sensitive analysis of the epigenome at single-cell resolution. This method is powered by the combination of both Bio-Rad’s droplet-based microfluidic and a hyperactive Tn5 t…

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RRBS service

Diagenode

Single-base resolution CpG detection with our popular Premium RRBS kit (internal bisulfite conversion controls included) Latest technology in bisulfite-seq using Illumina sequencers: > 10x coverage per CpG More than 4 million CpGs detected in human samples As little as 100 ng in mammals, birds, fish ,and more Complete end-to-end service: wet lab to bioinformatics for only 550 per sample!

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EPIC array service

Diagenode

The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allows to quantitatively detect the methylation level of over 850,000 human CpG positions throughout the genome with single nucleotide resolution.   Briefly, upon treatment with bisulfite, unmethylated cytosine bases are converted to uracil, while methylated cytosine bases remain…

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ChIP-seq service

Diagenode

Integrative or individual analyses for chromatin with ENCODE standards. End-to-end wet lab service and full ChIP-seq analysis Collaborative and customized project design to meet your needs Dedicated in-house expert Integrative epigenomics data analysis and presentation-ready graphs Expert, extensive bioinformatics

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