Drug discovery > Basic Research Products & Reviews

When performing two-color detection, loading controls and normalization are essential for reliable, precise comparison of protein levels. LICORbio offers primary antibodies for normalization that can be used for two-color normalization when performing multiplex Western blots or for cell-based assay normalization.

A low-background membrane is essential for infrared Western blot success. Background can be attributed to membrane autofluorescence or to detection of antibody non-specifically binding to the membrane. PVDF membranes included in LICORbio kits are screened to ensure low autofluorescence to ensure high signal to noise.

WesternSure ECL Stripping Buffer provides a robust solution for stripping primary and secondary antibodies from Western blot membranes while maintaining target antigen integrity for efficient reprobing.

NewBlot IR Stripping Buffer is ideal for stripping and reprobing nitrocellulose and PVDF membranes detected with IRDye ® secondary antibodies. It may also be suitable for other dye-conjugated secondary antibodies.

CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.

Chameleon®  Pre-Stained Protein Ladders offer easy visualization of gel migration and protein size, and can be used to orient your gel or membrane quickly. These versatile protein markers offer multi-colored, pre-stained proteins for both visual inspection and two-color near-infrared detection. Choose from either single-channel or two-channel detection.

The WesternSure Protein Ladder is the only pre-stained, multi-colored protein ladder that provides chemiluminescent signal for film and digital detection. It will fit with your current protocol without optimization and does not rely on strep-tagged HRP or secondary antibody binding. The WesternSure protein ladder works with a variety of substrates and provides a vibrant color scheme that allows you to quickly orient your membr…

The D-DiGit Gel Scanner offers complete nucleic acid gel analysis documentation without the hazards. Minimize exposure to hazards like UV light and ethidium bromide when image nucleic acid gels.

4X Protein Sample Loading Buffer is optimized for use as a loading buffer for protein gel electrophoresis. This orange loading buffer is recommended for use with Odyssey® Imaging Systems as it does not fluoresce in the 700 channel.

Chameleon Vue Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for molecular weight estimation