Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
Hamamatsu’s brilliantly designed ORCA-Flash4.0 is truly a game changer in the world of scientific imaging. Built on a revolutionary new Gen II sCMOS detector, the ORCA-Flash4.0 is the first sCMOS camera that challenges the performance of all CCD, EM-CCD, and Gen I sCMOS cameras. With its combination of low noise and high quantum efficiency, the ORCA-Flash4.0 delivers unprecedented sensitivity as well as high dynamic range, bla…
SeqSolve™ is an advanced and user-friendly software solution for the tertiary analysis of Next Generation Sequencing (NGS) data. By integrating state-of-the-art bioinformatics methods into one-click analysis workflows, SeqSolve is the only software able to provide scientists with such a combination of both cutting edge and intuitive user experience. Its Click And Go® technology enables the automatic processing of the data, whi…
5 mL capacity with round bottom Self-standing design Black graduations and larger marking spots for easier filling and sample identification Manufactured from polypropylene to withstand temperatures to -196°C Silicone washer for a secure seal Vials may be color coded with inserts (see Cat. No. 430499) Special base design allows vials to be locked into cryogenic rack and tray (Cat. No. 430525 or 431131) for single-hande…
An arrayed or non-arrayed BAC library is the preferred choice for downstream applications such as hybridization screening; full-genome sequencing; BAC end sequencing or physical mapping projects. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Deliverables for ARRAYED BAC library: Clones will be arrayed and amplifie…
A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. We typically construct a minimum 5X BAC library and BAC clones are then pooled and distributed in 96-well plates. Each well will contain about 400-500 original BAC clones. HMW DNA isolati…
Our uncloned normalized cDNA is created using proprietary technology which features full-length-enriched and high quality size-fractionated cDNA. Our cDNA has been used successfully with FLX, Titanium an Illumina sequencing.
Our cDNA libraries feature full-length-enriched and high quality size-fractionated cDNA. cDNA library construction is usually done in slightly-modified versions of pBluescript or pcDNA3.1. Insert sizes will range from about 0.5Kb to 4Kb. Concerning QC steps, we evaluate the average insert size, empty clone rate and clone concentration/tube before shipping out. We always provide a gel picture of randomly selected clones showing…
APAgene™ GOLD Genome Walking Kits are designed to rapidly and reliably amplify unknown genomic DNA using our patented APA technology: 1. Isolate unknown genomic sequences flanking transgenes (including T-DNAs, gene traps, transposons), sequence-tagged sites (STSs) or expressed sequence tags (ESTs). 2. Isolate localized sequences flanking known sequences from large clones and genomic DNAs. 3. Sequence insert ends of large clone…
ASH is APA-based, full-length-enriched, single-stranded cDNA subtraction hybridization library. - ASH cDNA subtraction is full-length-enriched and independent of PCR suppression. - ASH cloning is directional and does not involve normalization. - ASH method provides non-exponential amplification before subtraction because double-stranded cDNA is not required. - ASH method has a better subtraction effect because 1) a high ratio…
Bio S&T's high-quality RNA can be used directly for NxGen RNA sequencing as well as other downstream applications that require optimal-quality RNA such as quantitative real-time RT-PCR, microRNA cloning and amplification and Northern blotting.
