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The Click-iT Plus EdU Alexa Fluor 594 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed cova…

CellTrace CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace CFSE dye. Using this dye, eight or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of dist…

CellTrace Violet Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Violet dye. Using this dye, ten or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of di…

CellTrace Far Red Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Far Red dye. Using this dye, six or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of…

CellTrace Yellow Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Yellow dye. Using this dye, six or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of di…

CellTrace Blue Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Blue dye. Using this dye, five generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of distinct gene…

The LIVE/DEAD BacLight Bacterial Viability and Counting Kit allows researchers to reliably distinguish and quantitate live and dead bacteria with the aid of a flow cytometer, even in a mixed population containing a range of bacterial types. This kit uses a mixture of two nucleic acid stains - green-fluorescent SYTO™ 9 dye and red-fluorescent propidium iodide - for viability determinations, and a calibrated suspension of micros…

The LIVE/DEAD FungaLight Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO™ 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. SYTO 9 stain generally labels all yeast in a population, with or without intact membranes. Propidium iodide penetrates only…

The LIVE/DEAD BacLight Bacterial Viability Kit for microscopy and quantitative assays provides a convenient and easy-to-use solution for monitoring the viability of bacterial populations based on the membrane integrity of the cell. Cells with a compromised membrane that are considered to be dead or dying will stain red, whereas cells with an intact membrane will stain green.

The LIVE/DEAD Fixable Blue Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Blue Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells wit…

The LIVE/DEAD Fixable Violet Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Violet Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells…

The LIVE/DEAD Fixable Aqua Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Aqua Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells wit…

The LIVE/DEAD Fixable Yellow Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Yellow Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells…

The LIVE/DEAD Fixable Green Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Green Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells w…

The LIVE/DEAD Fixable Red Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Red Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells with…

The LIVE/DEAD Fixable Far Red Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Far Red Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cel…

Propidium Iodide Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. Propidium iodide can only enter cells that have lost membrane intergrity (e.g., dead cells). Once inside, it can bind with nucleic acids, emtting a red fluorescence, which has excitation/emission maxima of 535/617 nm. Propidium iodide is commonly excited with the 488 nm laser,…

TO-PRO3 Ready Flow Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. TO-PRO3 Ready Flow Reagent can only enter cells that have lost membrane intergrity (e.g., dead cells). Once inside, it can bind with nucleic acids, emitting a red fluorescence, which has an excitation/emission maxima of 642/661 nm, similar to Alexa Fluor™ 647 or allophycocyanin (APC).Fe…

SYTOX AADvanced Ready Flow Reagent is a high-affinity nucleic acid stain for the detection of dead cells in flow cytometry. SYTOX AADvanced Ready Flow Reagent can only enter cells that have lost membrane intergrity (e.g., dead cells). Once inside, it can bind with nucleic acids, emitting a red fluorescence, which has an excitation/emission maxima of 546/647 nm. The SYTOX AADvanced Ready Flow Reagent dye is spectrally similar t…

SYTOX™ Dead Cell Stain Sampler Kit contains sample sizes of five nucleic acid stains to identify the most appropriate satin to work in multi-color experiments. Stains include: SYTOX Blue (for the 405 nm violet laser), SYTOX Green (for the 488 nm blue laser with green emission), SYTOX Orange (for the 532 nm green laser), SYTOX AADvanced (for the 488 nm blue laser with red emission), and SYTOX Red (for the 633/5 nm red laser).St…

Annexin V Alexa Fluor 488 Ready Flow Conjugate is a bright, easy-to-use stain for apoptosis with high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. The Annexin V Alex Fluor 488 conjugate is excitable at 495 nm with an emission peak at 519 nm.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteCan be added directl…

Annexin V Alexa Fluor 647 Ready Flow Conjugate is a bright, easy-to-use stain for apoptosis with high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. The Annexin V Alex Fluor 647 conjugate is excitable at 650 nm with an emission peak at 665 nm.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteCan be added directl…

Annexin V APC Ready Flow Conjugate is a bright, easy-to-use stain for apoptosis with high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. The Annexin V APC conjugate is excitable at 650 nm with an emission peak at 660 nm.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteCan be added directly to Ca2+-containing me…

Annexin V Pacific Blue Ready Flow Conjugate is a bright, easy-to-use stain for apoptosis with high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. The Annexin V Pacific Blue conjugate is excitable at 410 nm with an emission peak at 455 nm.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteCan be added directly to…

Neurofilament light (NF-L) is a 68 kDa cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 125 kDa Neurofilament medium(NF-M) and the 200 kDa Neurofilament heavy (NF-H) to form neurofilaments. They are major components of the neuronal cytoskeleton, and are believed to function primarily to provide structural support for the axon and to regulate axon diameter. Neurofilaments can be re…