Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
R&D Human G-CSF Quantikine ELISA Kit - Human G-CSF Immunoassay for the quantitative determination of human granulocyte colony stimulating factor (G-CSF) concentrations in cell culture supernates, serum, and plasma. The R&D Quantikine G-CSF ELISA is a 3.5 - 4.5 hour solid phase ELISA designed to measure G-CSF in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human G-CSF and antibodies rai…
Guava Mitochondrial Depolarization Assay for detection of early apoptosis. There is growing interest within the scientific community to evaluate changes in the mitochondrial membrane potential and further define its role in initiating apoptosis, cell cycle, and other cellular processes. Automated single-cell analysis is the technique of choice due to its sensitivity and high reproducibility. Guava Technologies has optimized an…
The Guava Caspase Assay Suite of fluorescent-based assays for Caspase 3/7, 8, 9 and multi-caspase employ the same protocol making it easier to perform multiple caspase assays in parallel. Further, Guava caspase reagents are membrane permeable allowing one to measure intracellular levels of the enzyme without using harsh lysis methods. The Guava Caspase Assays employ a FLICA (Fluorescent Labeled Inhibitor of Caspase) reagent th…
R&D Proteome Profiler Human Phospho-RTK Array Kit - For the parallel determination of the relative level of tyrosine phosphorylation of receptor tyrosine kinases (RTKs). Proteome Profiler Human Phospho-RTK Array Kit contains 4 membranes - each spotted in duplicate with 42 different RTK antibodies. The R&D Human Phospho-Receptor Tyrosine Kinase (Phospho-RTK) Array is a rapid, sensitive, and economical tool used to detect chang…
Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate - Rat IL-1/IL-1F2 Immunoassay for the quantitative determination of rat interleukin 1 beta (IL-1ß) concentrations in cell culture supernates, rat serum, and rat EDTA plasma. The R&D Quantikine Rat IL-1 beta ELISA is a 4.5 hour solid phase ELISA. This R&D ELISA employs the quantitative sandwich enzyme immunoassay technique. R&D Quantikine ELISA Kits are complete and ready-to-…
Human Total MMP-9 Quantikine ELISA Kit- Human MMP-9 (total) Immunoassay for the quantitative determination of human active and pro-Matrix Metalloproteinase 9 (total MMP-9) concentrations in cell culture supernates, saliva, serum, plasma, and urine.The R&D Quantikine MMP-9 Immunoassay is a 3.5 hour solid phase ELISA. This R&D Quantikine assay employs the quantitative sandwich enzyme immunoassay technique. R&D Systems Quantikin…
R&D Human sVCAM-1/CD106 Quantikine ELISA Kit - Human sVCAM-1 Immunoassay for the quantitative determination of human soluble Vascular Cell Adhesion Molecule-1 (sVCAM-1) concentrations in cell culture supernates, serum and plasma. The Quantikine Human sVCAM-1 ELISA is a 2 hour solid-phase ELISA. This R&D Quantikine assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for sVCAM-1h…
R&D Human IGF-I Quantikine ELISA Kit - Human IGF-I Immunoassay for the quantitative determination of human insulin-like growth factor I (IGF-I) concentrations in cell culture supernates, serum, and plasma. R&D Systems Quantikine ELISA Kits are complete and ready-to-use. Immunoassays are available for measuring a range of molecules including cytokines, chemokines, growth factors, proteases, and more. The R&D Quantikine Human I…
R&D Systems Mouse/Rat/Porcine TGF-beta 1 Quantikine ELISA Kit - Mouse/Rat/Porcine/Canine TGF-beta Immunoassay for the quantitative determination of activated mouse/rat/porcine/canine Transforming Growth Factor beta 1 (TGF-ß 1) concentrations in cell culture supernates, serum, and plasma.The Quantikine TGF-beta ELISA is a 4.5 hour solid phase ELISA. This R&D quantikine assay employs the quantitative sandwich enzyme immunoassay…
R&D Human IFN-gamma Quantikine ELISA Kit - Human IFN-gamma Immunoassay for the quantitative determination of human interferon gamma (IFN-gamma) concentrations in cell culture supernates, serum, and plasma. The R&D Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure IFN-gamma levels in cell culture supernates, serum, and plasma. The Quantikine ELISA contains E. coli-expressed recombinant…
R&D Prostaglandin E2 Parameter ELISA Kit - For the quantitative determination of Prostaglandin E2 (PGE2) in cell culture supernates, serum, plasma, and urine. The R&D Parameter PGE2 ELISA is a 3.5 hour forward sequential competitive enzyme immunoassay designed to measure PGE2 in cell culture supernates, serum, plasma, and urine. This R&D Paramerter assay is based on the forward sequential competitive binding technique in whic…
R&D Proteome Profiler Human Phospho-Kinase Array Kit - Human Phospho-Kinase Array Kit for the parallel determination of the relative levels of protein phosphorylation. The kit contains 4 sets of 2 membranes - each spotted in duplicate with 46 different kinase antibodies. The R&D Human Phospho-Kinase Array is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of phosphorylation of 46 kinase ph…
R&D VEGF -Recombinant Human VEGF 165R Protein Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. Specifications and Use for R&D VEGF 165RSource of R&D VEGF• A DNA sequence encoding the 165 amino acid residue variant of human VEGF (Leung, D.W. et al., 1989, Science 246:1306) was expressed in…
R&D Activin - Recombinant Activin A Protein from Human/Mouse/Rat Activins and inhibins, members of the TGF-β superfamily, have been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and reproductive physiology. Specifications and Use for R&D Activin ASource of R&D Activin• A DNA sequence encoding the human Activin A protein sequenc…
R&D Systems Recombinant Human BMP-4 BMP-4 is a TGF-β superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Specifications and Use of R&D BMP-4R&D BMP-4 Source • A DNA sequence encoding the human BMP-4 mature chain (amino acid r…
R&D Recombinant Mouse Wnt-3a Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23-24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development. Specifications and Use for R&D Wnt-3aSource of R&D Wnt-3a • A DNA sequence encoding the signal peptide from human CD33, Met 1 - Ala 16, joined with the mature mouse Wnt-3A, Ser 19 - Lys 352 (Accession # P27467; Roelink, H.…
R&D Systems Recombinant Human IL-4 Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa - 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four α-helix structure. Specifications and Use of R&D Systems Recombinant Human IL-4Source • A DNA sequence en…
R&D TNF-alpha / TNFSF1A - Recombinant Human Protein Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. Specifications and Use for R&D TNF-alphaSource of R&D TNF• A DNA sequence encoding the mature human TNF-α protein sequence (Accession…
R&D Systems Recombinant Human FGF basic (146 aa) FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35 - 60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. Specifications and Use of R&D FGF basic Source of R&D FGF • A DNA sequenc…
R&D Systems Recombinant Human IL-6 Interleukin 6 (IL-6) is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic Specifications and Use of R&D IL-6 Source• A DNA sequence encoding the mature human IL-6 protein sequen…
R&D Systems Recombinant Human IGF-I/IGF-1, CF Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides. These isoforms are differentially expressed by various tissues. Specifications and Use of R&D Recombinant Human IGF-1 / ICF-1, CFSource…
R&D Systems Recombinant SCF Protein Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. Specifications and Use for R&D SC…
R&D Mouse TNF-alpha/TNFSF1A DuoSet This R&D DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Tumor Necrosis Factor alpha (TNF-alpha). DuoSets are designed for the analysis of cell culture supernates. Other sample types, such as serum and plasma, need to be validated prior to use in this R&D DuoSet. Each R&D DuoSet ELISA kit cont…
R&D SCF - Recombinant Mouse Protein Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. Specifications and Use for R&D SC…
R&D Systems Recombinant Human LDL RThe low density lipoprotein receptor (LDLR) is the founding member of the LDLR family of scavenger receptors. This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form β-propellers. Specifications and Use for R&D LDL RSource of R&D LDL R• A DNA sequence encoding the signal peptide from human CD33 was…
