Western Blotting Products & Reviews

The Axygen Power Supplies are full-featured power sources ideal for molecular biology workflows including DNA and protein gel applications. The compact user interface has 4 sets of color-coded terminals allowing for use with up to 4 gel boxes simultaneously. Depending on the model, operating modes include constant voltage, current, or power all with automatic crossover feature. The PS300GP can be quickly programmed to run with…

The Axygen Power Supplies are full-featured power sources ideal for molecular biology workflows including DNA and protein gel applications. The compact user interface has 4 sets of color-coded terminals allowing for use with up to 4 gel boxes simultaneously. Depending on the model, operating modes include constant voltage, current, or power all with automatic crossover feature. The PS250HA has the ability to set programs with…

The Axygen Power Supplies are full-featured power sources ideal for molecular biology workflows including DNA and protein gel applications. The compact user interface has 4 sets of color-coded terminals allowing for use with up to 4 gel boxes simultaneously. Depending on the model, operating modes include constant voltage, current, or power all with automatic crossover feature. The PS300GP can be quickly programmed to run with…

Biotin labelling of protein, amine reactive.  Only 1 hour to get conjugates, all processes in a single filtration tube, high recovery of conjugates, applicable for 50-200 ug IgG

Our Chemifluorescence Western Blotting kit allows sensitive, Chemifluorescent detection of specific Protein targets. Attomolar detection sensitivity Non-isotopic detection with low background Quantitation over four orders of magnitude Optimized for use with the FMBIO® Fluorescence Imaging System During a chemifluorescent assay, a nonfluorogenic substrate is coverted to a fluorescent product by an enzyme such as alkaline phos…

Our Chemifluorescence Western Blotting kit allows sensitive, Chemifluorescent detection of specific Protein targets. Attomolar detection sensitivity Non-isotopic detection with low background Quantitation over four orders of magnitude Optimized for use with the FMBIO® Fluorescence Imaging System During a chemifluorescent assay, a nonfluorogenic substrate is coverted to a fluorescent product by an enzyme such as alkaline phos…

Dedicated electroblotting for mini and maxi gels Recently redesigned to accommodate up to 4 electroblotting cassettes, the Scie-Plas TV100-EBK and TV400-EBK electroblotters provide a cost-effective and safe and easy to use system for the transfer of proteins and nucleic acids from 10 x 10cm mini and 20.5 x 20cm maxi gels. Both units are supplied with asymmetric colour-coded cassettes as a unique design feature to prevent them…

These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting, typically 15 to 30 minutes. All units can be used for all types of blotting: western, southern and northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Each unit is compatible with their respective omniPAGE vertical gel system. Semi Dry…

These Capillary Blotting systems offer a contained unit for southern and northern blotting. These units incorporate optimized permanent wicks which eliminate the requirement for overlapping wicks, offering greater consistency. The porous polyethylene platform draws up transfer buffer faster than traditional systems allowing for faster transfer times. Side by side transfer of multiple gels can be performed in a single set up. A…

Four different sample number size and style of Hybridisation Manifold are offered: two types of dot blotter and two types of slot blotter.Typical applications include clone screening with DNA / RNA probes in Southern / Northern blots and immunological screening with antibodies in western blots. The units incorporate precision lapped surfaces to ensure uniform blotting membrane contact and a leak proof gasket. These prevent lat…

ApplicationThe kit is used for the quantitative determination of BrdU incorporated into cellular DNA using a 96-well microplate cell ELISA format. Benefits Safer: No radioisotopes are used. Accurate: The results strongly correlate to those obtained with the [3H]-thymidine method. Sensitive: As sensitive as [3H]-thymidine. Fast: The use of multiwell ELISA readers allows for processing of a large number of samples. Ea…

The Western-Star™ system is a versatile, non-isotopic chemiluminescent immunodetection systems.

I-Block™ reagent is a highly purified casein-based blocking reagent.

The protein-free Blocking Buffer is a ready-to-use solution intended for blocking of the remaining free binding sites after membrane coating. The blocking is highly effective and stable.Our Synthetic Blocking Buffer™ eliminates the risk of false positive cross reactions in the assay. Current safety hazards related to the use of bovine material, for instance BSA are avoided. The buffer is ready-to-use and offers a high level of…

OPD, Ortho-Phenylenediamine, is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. The fizzing, buffer containing tablets are dissolved in distilled water within few minutes. This will give solution of pH 5.2…

OPD, Ortho-Phenylenediamine, is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. The fizzing, buffer containing tablets are dissolved in distilled water within few minutes. This will give solution of pH 5.2…

OPD is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. Stability life time is 18 months at 4°C. Our standard tablets are available with a content of 2 mg or 10 mg OPD.

AEC (3-amino-9-ethylcarbazole) is used as a chromogen substrate in immunoblotting and immunohistochemistry. AEC produces a rose-red colour during the enzymatic degradation of H2SO4 by horseradish peroxidase. Each tablet contains 5 mg of AEC and dissolves in approx. 5 minutes. 1 tablet is added 1 ml of 96 % alcohol, followed by addition of distilled water to 10 ml. This will give a solution of pH 5.1 + 0.1 buffered with 20 m…

DAB (3,3-diaminobenzidine tetrahydrochloride), is used as a chromogen substrate in immunoblotting.DAB produces a brown colour during the enzymatic degradation of H2SO4 by horseradish peroxidase. Each tablet contains 10 mg of DAB and dissolves in distilled water in less than 5 minutes. Stability life time is 18 months at 4°C.

Fast Red is used as a chromogen substrate in immunoblotting and immunohistochemistry using alkaline phosphatase antibody conjugates. Each tablet contains 0.5 mg naphthol phosphate, 2 mg FAST RED chromogen and 0.4 mg levamisole, an inhibitor of endogenous enzyme activity. Stability life time is 18 months at 4°C.

The pNPP (p-NitroPhenyl Phosphate) liquid, ready-to-use substrate is a highly active and stable formulation utilized for measuring AP- (Alkaline Phosphatase) probe activity. The solution is a solvent free buffer solution containing stabilized pNPP using sodium azide as preservative. In kinetic assays p-nitrophenol appears yellow and is measured at 405 /650 nm. If stopped reactions are preferred, add equal amounts of 1 .0 M s…

The liquid BCIP/NBT is the most commonly used substrate for colorimetric determination of Alkaline Phosphatase (AP) activity in membrane tests. We offer an aqueous buffered solution containing NBT (NitroBlue Tetrazolium) and BCIP (Bromo-Chloro-Indolyl Phosphate) forming a blue/purple precipitate at the positive sites. The colour develops when AP catalyses the dephosphorylation of BCIP. The intense blue/purple precipitate is…

TMB (3,3´,5,5´- tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP). Our mono-component, liquid TMB solutions combine TMB, substrate buffer and hydrogen peroxide for a safe, ready-to-use, one step, chromogen system. The products do not contain harmful solvents. Recommended for ELISA procedures, liquid TMB yields a soluble blue end product read at 655 nm. The TMB reaction may be stopped with 0.2 M H2SO4 resu…

This new type of TMB with a red indicator combines all the important qualities of TMB ONE, eliminating possible mistakes during pipetting. Further the red colour may be useful in automatic analyzers giving the warning "Reagent low" when it is time for filling up the substrate.The red indicator disappears after addition of the stopping solutionand will neither affect the sensitivity nor the stability of the substrate. Stability…

The TMB PLUS has a higher activity level, resulting in a higher sensitivity and a reduced incubation time than found using the TMB ONE. TMB is a substrate for HRP. The mono-component, liquid TMB solution combines TMB, substrate buffer and hydrogen peroxide for a safe, ready-to-use, one step, chromogen system.The product does not contain harmful solvents. Recommended for ELISA procedures, liquid TMB yields a soluble blue end pr…