Gene Expression and Molecular Cloning Products & Reviews

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Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Lambda FIX II/Xho I Partial Fill-in Vector

Agilent Technologies

Agilent Lambda FIX II/Xho I Partial Fill-in Vectors offer traditional genomic cloning of samples containing from 9 to 23 KB fragments. All of our vectors for genomic library construction contain T3 and T7 RNA promoters to ensure rapid gene walking and high-resolution gene mapping.

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Lambda ZAP -CMV XR Vector Gigapack Kit

Agilent Technologies

The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved blue-white color selection.  High-efficiency lambda cloning with versatili…

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Lambda ZAP II Vector Kit

Agilent Technologies

The Lambda ZAP II vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. They enable prokaryotic expression of your gene of interest under control of the lac promoter. In vivo excision of the Lambda ZAP II vector yields the pBluescript SK- vector, which is a high-copy number pUC-based phagemid.  

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Lambda ZAP II/EcoR I/CIAP Treated Vector

Agilent Technologies

The Lambda ZAP II vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. They enable prokaryotic expression of your gene of interest under control of the lac promoter. In vivo excision of the Lambda ZAP II vector yields the pBluescript SK- vector, which is a high-copy number pUC-based phagemid.  

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pAAV-hrGFP Vector

Agilent Technologies

The pAAV-hrGFP vector is used as a positive control in the AAV Helper-Free System. This control vector uses humanized Renilla GFP (hrGFP) reporter gene for easy detection of expression in vivo using fluorescence microscopy, fluorescence-activated cell sorting (FACS) analysis, or fluorometry. 

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pAAV-IRES-hrGFP Vector

Agilent Technologies

The pAAV-hrGFP vector is used as a positive control in the AAV Helper-Free System. This control vector uses humanized Renilla GFP (hrGFP) reporter gene for easy detection of expression in vivo using fluorescence microscopy, fluorescence-activated cell sorting (FACS) analysis, or fluorometry.

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pAdEasy-1 Vector

Agilent Technologies

Recombinant adenoviruses are a versatile tool for gene delivery and expression. Choose pAdEasy-1 vector or any of these three shuttle vectors, pShuttle, pShuttle-CMV, or pShuttle-CMV-lacZ vector, to clone DNA of interest with the AdEasy adenoviral vector system.

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PathDetect c/EBP Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect DR1/RXRE Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect DR3/VDR Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect DR4/TR Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect DR5/RARE Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect Egr-1 Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect GRE Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect LILRE Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect NFAT Cis-Reporter Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect p53-Luc Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect pAP-1-hrGFP Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect pAP1-Luc Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect pCIS-CK Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect pCRE-hrGFP Cis-Reporting Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect pCRE-Luc Plasmid

Agilent Technologies

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.  Identify pathway-specific transcription activation  Easier,…

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PathDetect pFA-ATF2 Plasmid

Agilent Technologies

The PathDetect in vivo signal transduction pathway trans-reporting systems are designed for specific, rapid, and convenient assessment of the in vivo activation of transcription activators and upstream signal transduction pathways. The systems are useful for identifying whether a newly cloned gene is involved in a signal transduction pathway, and if so, at which step of the pathway.  Identify specific stimuli that leads to th…

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