Gene Expression and Molecular Cloning Products & Reviews

The TOPO™ XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb).

The second generation of our QuikChange method that provides improved fidelity over our original kit, while maintaining greater than 80% mutation efficiency for single site mutagenesis.

The second generation of our QuikChange method that provides improved fidelity over our original kit, while maintaining greater than 80% mutation efficiency for single site mutagenesis.

The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase. These kits are available for both blunt-end and UA cloning. 

Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.

The original QuikChange Site-Directed Mutagenesis Kits eliminate the need for subcloning into M13-based bacteriophage vectors and for ss-DNA rescue. This makes site-directed mutagenesis studies simple and reliable since it allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. 

The High Sensitivity ß-Galactosidase Assay Kit provides an easy, rapid, and sensitive method for determining the ß-galactosidase activity in the lysates of cells transfected with a ß-galactosidase expression construct. Rapid, simple and sensitive >= 10-fold increase in sensitivity over ONPG-based kits Reduces amount of cell extract needed for assay Useful for cells that are difficult to transfect and have low ß-gal expression…

Human iPSC-derived glutamatergic neurons expressing Cas9 for rapid gene knockout generation

UltraScript® Reverse Transcriptase is a robust and thermostable modified MMLV reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation.

Broad portfolio of products for knockdown of gene expression

Morphology driven high plex profiling on a single tissue section

Double-stranded DNA fragments for high-throughput screening applications

The Model P-97 micropipette puller is the latest generation of the Flaming/Brown type puller for fabrication of micropipettes, patch pipettes and microinjection needles. While retaining many of the features on the Model P-87, the P-97 offers improvements in mechanical, electronic and software design. The result is better control of the pulling process and a higher degree of reproducibility. Features: Environmental chamber…

Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high quality DNA synthesis and assembly; qualities that allow Twist to create perfect genes of various lengths and difficulty, NGS-verified and delivered on-time when you need them.

Twist Oligo Pools are highly diverse collections of single-stranded oligonucleotides synthesized using our silicon-based DNA writing technology. Our synthesis platform enables massively parallel production of hundreds of thousands of high-quality, accurate oligos per run.

Twist Bioscience Gene Fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.

Unleash the full power of a well-designed library. With Twist Bioscience, you are assured that the library you design contains the specific modifications, exactly where you want them, saving you valuable resources for the downstream screening process.

The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high- and ultrahigh-throughput applications, the ONE-Glo™ Assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor than standard luciferase assay reagents…

jetPRIME™ is a new versatile and powerful DNA and siRNA transfection reagent for day-to-day experiments. jetPRIME™ ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells and is ideal for DNA/siRNA co-transfection. jetPRIME™ is very gentle to cells since it requires low amounts of reagent and nucleic acid during transfection. Effective and nontoxic DNA and siRNA delivery is essentia…

shRNAmir-GFP: Visualize RNAi Thermo Scientific GIPZ Lentiviral shRNAmir advantages: shRNAmir design for specificity and increased knockdown efficiency TurboGFP marks cells expressing shRNAmir Efficient low copy knockdown- Important for pooled screens Genome-wide human and mouse coverage Perform efficient RNAi in primary and non-dividing cells Guaranteed* knockdown Please note that the pGIPZ and pTRIPZ vectors a…

The LightSwitch 3’UTR reporter GoClone collection includes transfection-ready luciferase reporter constructs utilizing the novel RenSP luciferase technology for over 12,000 human 3’UTRs and highly optimized LightSwitch reagents. Measure RNA stability, translation efficiency and the functional impact of miRNAs on a gene-by-gene basis. Measuring 3’UTR activity characterizes: The impact of the UTR in post-transcriptional gene…

A high performance, animal-free, broad spectrum DNA transfection reagent. Broad Spectrum DNA Delivery - Achieve high expression in many cell types, including hard to transfect cell lines and primary cells Outperforms Competitor Reagents - TransIT®-2020 demonstrated higher protein yield when compared to FuGENE® HD, Lipofectamine™ 2000, and Lipofectamine™ 2000 CD Superior Transfection of Insect Cells - Obtain higher expre…

TaqMan® Gene Expression Assays are a comprehensive collection of over 1,000,000 probe and primer sets for quantitative gene expression analysis of 13 species including human, mouse, rat, Arabidopsis, Drosophila, and C. elegans genes. These assays let you quickly, easily, and affordably perform precise gene expression studies using real-time PCR. TaqMan®-based assays provide unmatched sensitivity, specificity, and reproducibi…

Lateral flow DNA detection

Real-time fluorescent RNA detection