Gene Expression and Molecular Cloning Products & Reviews

Human iPSC-derived glutamatergic neurons expressing Cas9 for rapid gene knockout generation

The TOPO™ XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb).

UltraScript® Reverse Transcriptase is a robust and thermostable modified MMLV reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation.

Morphology driven high plex profiling on a single tissue section

Double-stranded DNA fragments for high-throughput screening applications

The new InjectMan® 4 is ideal for microinjection into adherent cells, smaller organisms and embryos in the early stages of development. The combination of InjectMan 4, FemtoJet® 4i or 4x enables a fast, semi-automatic injection. Furthermore, the InjectMan 4 is the ideal micromanipulator for all complex applications that require a dynamic movement mode and direct control of the injection process via the joystick button. The ax…

The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high- and ultrahigh-throughput applications, the ONE-Glo™ Assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor than standard luciferase assay reagents…

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes).   Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification pri…

ViaFect™ Transfection Reagent allows high-efficiency transfection of a wide range of cell types, including iPS Cells, without compromising cell viability.   ViaFect™ Transfection Reagent enables the design of biologically relevant assays in workhorse adherent cell models, suspension cells important for studying cell signaling, and even differentiated cell lines derived from stem cells. ViaFect™ Transfection Reagent offer…

Capped or Uncapped RNA Transcripts for in vitro or in vivo Translation

MOA-based functional bioassay to measure Fc effector activity.

A best-in-class DNA transfection reagent for your research.

The pGEM®-T Easy Vector Systems are convienent systems to clone PCR products.   They offer all of the advantages of the pGEM®-T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM(R)-T Easy Vectr System II contains JM109 Competent Cells in addition to all pf the…

Broad portfolio of products for knockdown of gene expression

Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high quality DNA synthesis and assembly; qualities that allow Twist to create perfect genes of various lengths and difficulty, NGS-verified and delivered on-time when you need them.

Twist Oligo Pools are highly diverse collections of single-stranded oligonucleotides synthesized using our silicon-based DNA writing technology. Our synthesis platform enables massively parallel production of hundreds of thousands of high-quality, accurate oligos per run.

Twist Bioscience Gene Fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.

Unleash the full power of a well-designed library. With Twist Bioscience, you are assured that the library you design contains the specific modifications, exactly where you want them, saving you valuable resources for the downstream screening process.

jetPRIME™ is a new versatile and powerful DNA and siRNA transfection reagent for day-to-day experiments. jetPRIME™ ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells and is ideal for DNA/siRNA co-transfection. jetPRIME™ is very gentle to cells since it requires low amounts of reagent and nucleic acid during transfection. Effective and nontoxic DNA and siRNA delivery is essentia…

shRNAmir-GFP: Visualize RNAi Thermo Scientific GIPZ Lentiviral shRNAmir advantages: shRNAmir design for specificity and increased knockdown efficiency TurboGFP marks cells expressing shRNAmir Efficient low copy knockdown- Important for pooled screens Genome-wide human and mouse coverage Perform efficient RNAi in primary and non-dividing cells Guaranteed* knockdown Please note that the pGIPZ and pTRIPZ vectors a…

The second generation of our QuikChange method that provides improved fidelity over our original kit, while maintaining greater than 80% mutation efficiency for single site mutagenesis.

The second generation of our QuikChange method that provides improved fidelity over our original kit, while maintaining greater than 80% mutation efficiency for single site mutagenesis.

The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase. These kits are available for both blunt-end and UA cloning. 

Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.

The LightSwitch 3’UTR reporter GoClone collection includes transfection-ready luciferase reporter constructs utilizing the novel RenSP luciferase technology for over 12,000 human 3’UTRs and highly optimized LightSwitch reagents. Measure RNA stability, translation efficiency and the functional impact of miRNAs on a gene-by-gene basis. Measuring 3’UTR activity characterizes: The impact of the UTR in post-transcriptional gene…