ADME-toxicology (ADME-Tox) studies are used in pharmacology and pharmacokinetics to assess the activity/toxicity of drugs <i>in vivo</i> or <i>in vitro</i>. Find bioassays for absorption, distribution, metabolism, and excretion of drug molecules including cytotoxicity, transporter/permeability, metabolism and activity assays as well as hepatocytes and cell lines for ADME. Find the best ADME-toxicology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-9 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the LEHD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate LEHD-pNA. The free pNA can be quantified using a spectropho…
The CHEMICON Glutathione Detection Kit is a quick method for detection of intracellular levels of reduced glutathione (GSH). Reduction in levels of GSH may be an early indicator of apoptosis.
MTT is a pale yellow substrate that is cleaved by living cells to yield a dark blue formazan product. This process requires active mitochondria, and even freshly dead cells do not cleave significant amounts of MTT. The colorimetric assay described below can be used for either proliferation or complement-mediated cytotoxicity assays.
CHEMICON®'s Osteogenesis Quantitation Kit provides all the necessary reagents and a protocol to enable quantification of osteogenesis using a standard plate reader. This product is useful for studying the effects of growth factors, drugs and toxic agents on bone formation and for screening candidate osteogenic pharmaceuticals. Also, studies examining the intracellular signaling pathways regulating osteoblast differentiation ca…
The UPSTATE Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts. This assay combines the principle of the electrophoretic mobility shift assay (EMSA) with the 96-well based enzyme-linked immunosorbent assay (ELISA), enabling manual or high-throughput sample analysis with greater sensitivity t…
ToxCount™ provides a two wavelength cell viability assay based on determination of live and dead cells. The assay uses fluorescent dyes that measure permeability of the plasma membrane of dying cells and the ability of living cells to retain intracellular esterases, respectively. Live cells are distinguished by the presence of ubiquitous intracellular esterase activity, determined by the enzymatic conversion of the non fluores…
Comet Assay IV's unique single-click scoring method and ultra-fast live video picture make it the most efficient and easy-to-use system available for measuring DNA damage using single cell gel electrophoresis. There are no complicated parameters to adjust and no hardware to install - simply connect the camera and start scoring. Working closely with leading experts in the field, Perceptive Instruments have released Comet Assa…
The In Situ beta-Galactosidase Staining Kit provides an easy and rapid method to determine transfection efficiency by indicating beta-Galactosidase activity in individual intact cells. In situ staining of cells directly in dishes without removing or manipulating cells Rapid detection of Ÿ-gal activity in transfected cells Results in 24 hours Maximum signal with low background Deep blue staining reaction Kit in…
The Thermo Scientific ToxInsight Drug Induced Liver Injury Assay Cartridge for the ToxInsight IVT Platform offers a new multi-parameter cell-based assay to determine the risk of liver injury by chemicals being tested for new drugs or for consumer products safety assessment. The ToxInsight DILI Cartridge is for identifying compound hepatotoxicity by simultaneous detection of multiplexed targets, including cell loss, nuclear DNA…
The Thermo Scientific ToxInsight Endocrine Profiler Panel offers a complete solution for consumer products companies needing to comply with regulatory Reproductive Toxicity testing requirements. Traditional methods of assessing endocrine disruption in vitro measure single targets in homogeneous formats and have a record of low predictive power. The Thermo Scientific ToxInsight Endocrine Profiler Panel leverages our proven tech…
