Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element. Identify pathway-specific transcription activation Easier,…
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element. Identify pathway-specific transcription activation Easier,…
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element. Identify pathway-specific transcription activation Easier,…
The pBC vectors were derived from the pBluescript II phagemid. The ampicillin-resistance gene has been replaced with the chloramphenicol resistance gene. pBC phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and g…
The pBC vectors were derived from the pBluescript II phagemid. The ampicillin-resistance gene has been replaced with the chloramphenicol resistance gene. pBC phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and g…
The pBC vectors were derived from the pBluescript II phagemid. The ampicillin-resistance gene has been replaced with the chloramphenicol resistance gene. pBC phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and g…
The pBC vectors were derived from the pBluescript II phagemid. The ampicillin-resistance gene has been replaced with the chloramphenicol resistance gene. pBC phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and g…
The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector.?
Agilent pBlueScript II Vectors are powerful cloning vectors for a range of research applications. Featuring an extensive polylinker with 21 unique restriction enzyme recognition sites, the vectors are suitable for a range of DNA sequencing and cloning processes.
Agilent pBlueScript II Vectors are powerful cloning vectors for a range of research applications. Featuring an extensive polylinker with 21 unique restriction enzyme recognition sites, the vectors are suitable for a range of DNA sequencing and cloning processes.
The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin (available separately). pCAL vectors derived from T7 RNA polymerase-based pET vectors€_€_ Novel purification tag combines small size, gentle el…
The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin (available separately). pCAL vectors derived from T7 RNA polymerase-based pET vectors€_€_ Novel purification tag combines small size, gentle el…
The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin (available separately). pCAL vectors derived from T7 RNA polymerase-based pET vectors€_€_ Novel purification tag combines small size, gentle el…
The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin (available separately). pCAL vectors derived from T7 RNA polymerase-based pET vectors€_€_ Novel purification tag combines small size, gentle el…
Dose-responsive inducible mammalian expression Induction as high as 1,700 fold Tightly repressed Novel IRES site allows expression of both receptor subunits from the same CMV promoter Retroviral and plasmid gene delivery systems available Stable receptor cell lines available
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study.
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-3Tag vectors are a series of epitope tagging mammalian expression vectors featuring three copies of the epitope tag in a variety of configurations. pCMV-Tag vectors have a single copy of the available epitope tags, resulting in weaker tagging. Stronger signal in immunological assays Small tags do not interfere with functional analysis studies Three reading frames for both N- and C- terminus tags
The pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1 hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. The pCMV-Script vector is derived from a high-copy-number pUC-based plasmid and is designed to allow protein expression in mammalian systems. Mammalian expression…
The pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1 hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.
