Gene Expression and Molecular Cloning Products & Reviews

The Agilent Model Organism Genome CGH Microarray brings optimal clarity to comparative genomic hybridization by enabling researchers to perform ultra high resolution, genome-wide DNA variation profiling without amplification or complexity reduction.  Coding and non-coding sequences represented  Format-dependent overall median probe spacing  Probes annotated against indicated sources  0.5g total genomic DNA input require…

The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved blue white color selection.  High-efficiency lambda cloning with versatili…

Lambda DASH II vector kits offer traditional genomic cloning of 9−23 Kb fragments. All of our vectors for genomic library construction contain T3 and T7 RNA promoters for rapid gene walking and high-resolution gene mapping.

The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved blue-white color selection.  High-efficiency lambda cloning with versatili…

Lambda DASH II vector kits offer traditional genomic cloning of 9−23 Kb fragments. All of our vectors for genomic library construction contain T3 and T7 RNA promoters for rapid gene walking and high-resolution gene mapping.

The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved blue-white color selection.  High-efficiency lambda cloning with versatili…

The Lambda ZAP II vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. 

The Lambda ZAP-CMV XR library construction kit uses a hybrid oligo(dT) linkerprimer that contains an Xho I restriction site. Messenger RNA is primed in the first-strand synthesis with the linkerprimer and is then reverse transcribed.  Create large, highly-representational libraries  Directional cloning reduces background  Easy to amplify and screen your library  Clone genes that are toxic in E.coli  Rapidly convert fr…

The Lambda ZAP-CMV XR library construction kit uses a hybrid oligo(dT) linkerprimer that contains an Xho I restriction site. Messenger RNA is primed in the first-strand synthesis with the linkerprimer and is then reverse transcribed.  Create large, highly-representational libraries  Directional cloning reduces background  Easy to amplify and screen your library  Clone genes that are toxic in E.coli  Rapidly convert fr…

The MycoSensor PCR Assay Kits detect Mycoplasma infection of cell cultures by PCR in less than two hours.

The MycoSensor PCR and qPCR assay kits detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) or PCR in less than two hours. The easy-to-use system can specifically detect the eight most common Mycoplasma species.

The MycoSensor PCR and qPCR assay kits detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) or PCR in less than two hours. The easy-to-use system can specifically detect the eight most common Mycoplasma species.

The VariFlex protein expression system offers vectors with unique tags that enhance the utility of E.coli as an expression host. Included in our tag offering are three different SET tags which increase protein solubility and the SBP purification tag for high-yield, pure proteins.  Unique SET tags increase protein solubility  All three SET tags in every vector set  Gentle SBP purification tag generates high-yield, pure pro…

The VariFlex protein expression system offers vectors with unique tags that enhance the utility of E.coli as an expression host. Included in our tag offering are three different SET tags which increase protein solubility and the SBP purification tag for high-yield, pure proteins.  Unique SET tags increase protein solubility  All three SET tags in every vector set  Gentle SBP purification tag generates high-yield, pure pro…

The VariFlex protein expression system offers vectors with unique tags that enhance the utility of E.coli as an expression host. Included in our tag offering are three different SET tags which increase protein solubility and the SBP purification tag for high-yield, pure proteins.  Unique SET tags increase protein solubility  All three SET tags in every vector set  Gentle SBP purification tag generates high-yield, pure pro…

The ViraPack Transfection Kit is based on a modified CaPO4 method of DNA transfection to introduce viral plasmids into HEK293 packaging cell lines with high efficiency.

Three different strains that provide varying levels of expression control with T7 promoter-driven vectors, such as the pCAL and pET vectors.  BL21 derivatives for tight expression control  Express toxic proteins  Use BL21, BL21-Gold or BL21-CodonPlus cells  High-level protein expression  Use for established expression constructs  Economical alternative

The Lambda ZAP II vector kit for rapid excision. These vectors enable prokaryotic expression of your gene of interest under control of the lac promoter. In vivo excision of the Lambda ZAP II vector yields the pBluescript SK- vector, which is a high-copy number pUC-based phagemid.

Helper phages for mass excision of Lambda ZAP, ZAP Express libraries, and for single strand rescue protocols.  Use for mass excision of Lambda ZAP®, and ZAP Express® libraries  Interference-resistant helper phage  Eliminates the possibility of co-infection from helper phage

The ExAssist helper phage with XOLR works with the Lambda expression vector system kits for mass excision of libraries in ZAP Express or Lambda ZAP vectors. The pCMV-Script or pBluescript phagemid will grow.

Agilent provides a range of Interference-resistant Helper Phages that enable ssDNA recovery from phagemid vectors. The pBlueScript II Phagemid Vector systems are available in R408 and VCSM13 format. R408 Interference-Resistant Helper Phages are stable and easy to grow, and are provided in a single-strand size of ~4 KB.

Helper phages for mass excision of Lambda ZAP, ZAP Express libraries, and for single strand rescue protocols.  Use for mass excision of Lambda ZAP®, and ZAP Express® libraries  Interference-resistant helper phage  Eliminates the possibility of co-infection from helper phage

Agilent miRNA qPCR Detection Reagents are high-specificity reagents for the polyadenylation of microRNAs (miRNAs) from total RNA. The miRNA qPCR Detection Reagents also enable the synthesis of first-strand cDNA from the tailed miRNAs.

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.  New cDNA synt…

pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.  CMV promoter for high-level protein expression in mammalian cells  Large multiple cloning site contains 15 unique sites  Clone PCR products generated with any PCR e…