Gene Expression and Molecular Cloning Products & Reviews

Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.

Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.

The MBS Mammalian Transfection Kit increases transfection efficiencies by up to 100-fold. Cells are incubated with CaPO4/DNA coprecipitate for only 3 hours compared to 6 to 12 hours with traditional CaPO4 methods. 

The In Situ beta-Galactosidase Staining Kit provides an easy and rapid method to determine transfection efficiency by indicating beta-Galactosidase activity in individual intact cells.  In situ staining of cells directly in dishes without removing or manipulating cells  Rapid detection of Ÿ-gal activity in transfected cells  Results in 24 hours  Maximum signal with low background  Deep blue staining reaction  Kit in…

Our single-stranded DNA binding standard is a helix-destabilizing E. coli protein. The 75.6-kD protein is essential for replication, recombination, and repair processes.

The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD).  Detects protein-prote…

The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.  Tightly controlled T7 RNA polymerase-based gene expression in     E.coli    High-level expression upon induction  Convenien…

The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.  Tightly controlled T7 RNA polymerase-based gene expression in     E.coli    High-level expression upon induction  Convenien…

The InterPlay adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

The InterPlay adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression. The establishment of a highly regulated expression system in eukaryotic cells is an invaluable tool for the study of cell cycle, oncogenicity, cytogenicity, and the mechanisms of gene regulation.

The Luciferase Assay Kit provides rapid, sensitive, and quantitative measurement of American firefly (Photinus pyralis) luciferase activity in cultured mammalian cells.  Sensitive, widely used reporter assay  Rapid, quantitative results  Reporter activity immediately available upon translation  Useful in conjunction with PathDetect systems

The pDual and pDual GC expression vectors are designed for high-level expression of heterologous genes in both eukaryotic and prokaryotic systems.High-level protein expression in bacterial or mammalian systems Saves time by eliminating the need to subclone Express protein as a native or tagged fusion protein Epitope tagged for simple detection and affinity purification Clone seamlessly without compatible restriction sites Avai…

SureSelect Cancer CGP assay offers comprehensive genomic profiling for solid tumors. This pan-cancer NGS panel enables detection of somatic variants, including single nucleotide variants (SNVs), copy number variants (CNVs), insertions/deletions (indels), translocations (TLs), gene fusions, and the immuno-oncology biomarkers TMB (tumor mutational burden) and MSI (microsatellite instability).

The Agilent SurePrint G3 Human GE 8x60K Microarrays offer the same high sensitivity and accuracy provided by the earlier generation arrays but adds the benefits of extended coverage, greater throughput, and increased cost savings. The new SurePrint G3 Human GE 8x60K Microarrays and Human GE 4x44K v2 Microarrays are based on updated transcriptome databases for mRNA targets, while the SurePrint G3 arrays also include probes for…

Amplify a CRISPR guide sequence derived from a CROP-style vector alongside your standard RNA Sequencing Library.

UltraScript® cDNA Synthesis Kit Separate Oligos contains all the components for efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. Anchored oligo(dT) and random hexamers are provided separately for user optimisation depending on the type of analysis needed.

UltraScript 2.0 cDNA Synthesis Kit Separate Oligos containins all the components required for fast, reliable and unbiased cDNA synthesis of the most challenging RNA sample types. Anchored oligo(dT) and random hexamers are provided separately for user optimisation depending on the type of analysis needed.

UltraScript® 2.0 cDNA Synthesis Kit is a 2-tube kit containing all the components required for fast, reliable and unbiased cDNA synthesis of the most challenging RNA sample types.

UltraScript® 2.0 Reverse Transcriptase is a highly thermostable reverse transcriptase designed for cDNA synthesis of the most challenging RNA sample types. This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55 °C, giving improved specificity, higher cDNA yields and more full length cDNA product.

UltraScript® cDNA Synthesis Kit is an easy-to-use 2-tube system specifically developed to generate cDNA for use in real-time PCR. The kit provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.

Get ultra high yields with MEGAscript™ kits designed for the synthesis of large amounts of unlabeled or low specific activity RNA for in vivo or in vitro translation studies.

Predesigned probe assays for gene expression analysis

Modulation of gene expression with modified oligos

Unleash the full power of a well-designed library. With Twist Bioscience, you are assured that the library you design contains the specific modifications, exactly where you want them, saving you valuable resources for the downstream screening process.