RNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.
Agilent’s Quick Amp Labeling Kits reliably amplify and label target RNA for the robust generation of cRNA. Sample input of 200 ng total RNA Multiple kit formats available with one- or two-color labeling protocols Includes reagents for 20 reactions For Research Use Only. Not for Use in Diagnostic Procedures.
Agilent’s Quick Amp Labeling Kits reliably amplify and label target RNA for the robust generation of cRNA.
Agilent’s Quick Amp Labeling Kits reliably amplify and label target RNA for the robust generation of cRNA.
The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli.
The Big Blue Lambda LIZ (LacI/Z) shuttle vector is the bacteriophage lambda vector used in the Big Blue transgenic rodent mutation assay. The utility of this vector is its ability to act as a target for mutations in vivo that can subsequently be shuttled to E.coli for analysis. Ideal shuttle vector for any plaque assay Suitable for standard CaPO4 transformations Available to researchers for transfection of existing cel…
The Complete Control Inducible Mammalian Expression System provides the tightest control of expression available in mammalian cells.
InterPlay Adenoviral C-Terminal Vector, part of the InterPlay Adenoviral TAP System, combines Agilent’s unique tandem affinity purification system with our exclusive adenoviral gene delivery system for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
InterPlay Adenoviral C-Terminal Vector, part of the InterPlay Adenoviral TAP System, combines Agilent’s unique tandem affinity purification system with our exclusive adenoviral gene delivery system for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
The InterPlay Mammalian TAP System allows you to recover interacting proteins from mammalian cells. This method is based on expression of a protein of interest fused to two affinity tags: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP).
The InterPlay Mammalian TAP System allows you to recover interacting proteins from mammalian cells. This method is based on expression of a protein of interest fused to two affinity tags: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP).
Lambda DASH II vector kits offer traditional genomic cloning of 9−23 Kb fragments. All of our vectors for genomic library construction contain T3 and T7 RNA promoters for rapid gene walking and high-resolution gene mapping.
Lambda DASH II vector kits offer traditional genomic cloning of 9−23 Kb fragments. All of our vectors for genomic library construction contain T3 and T7 RNA promoters for rapid gene walking and high-resolution gene mapping.
Lambda DASH II vector kits offer traditional genomic cloning of 9−23 Kb fragments. All of our vectors for genomic library construction contain T3 and T7 RNA promoters for rapid gene walking and high-resolution gene mapping.
Agilent Lambda FIX II/Xho I Partial Fill-in Vectors offer traditional genomic cloning of samples containing from 9 to 23 KB fragments. All of our vectors for genomic library construction contain T3 and T7 RNA promoters to ensure rapid gene walking and high-resolution gene mapping.
Learn more about using Lambda gt11 Vector Kits to construct cDNA libraries. The Lambda gt11 expression vector accepts inserts up to 7.2 kilobases at a unique EcoR I site near the C-terminal end of the coding region of the lacZ gene.
The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved blue-white color selection. High-efficiency lambda cloning with versatili…
The Lambda ZAP II vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. They enable prokaryotic expression of your gene of interest under control of the lac promoter. In vivo excision of the Lambda ZAP II vector yields the pBluescript SK- vector, which is a high-copy number pUC-based phagemid.
The Lambda ZAP II vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems. They enable prokaryotic expression of your gene of interest under control of the lac promoter. In vivo excision of the Lambda ZAP II vector yields the pBluescript SK- vector, which is a high-copy number pUC-based phagemid.
The pAAV-hrGFP vector is used as a positive control in the AAV Helper-Free System. This control vector uses humanized Renilla GFP (hrGFP) reporter gene for easy detection of expression in vivo using fluorescence microscopy, fluorescence-activated cell sorting (FACS) analysis, or fluorometry.
The pAAV-hrGFP vector is used as a positive control in the AAV Helper-Free System. This control vector uses humanized Renilla GFP (hrGFP) reporter gene for easy detection of expression in vivo using fluorescence microscopy, fluorescence-activated cell sorting (FACS) analysis, or fluorometry.
Recombinant adenoviruses are a versatile tool for gene delivery and expression. Choose pAdEasy-1 vector or any of these three shuttle vectors, pShuttle, pShuttle-CMV, or pShuttle-CMV-lacZ vector, to clone DNA of interest with the AdEasy adenoviral vector system.
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element. Identify pathway-specific transcription activation Easier,…
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element. Identify pathway-specific transcription activation Easier,…
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element. Identify pathway-specific transcription activation Easier,…
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element. Identify pathway-specific transcription activation Easier,…
