Protein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
PhosphoSafe™ Extraction Buffer extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. The reagent, an enhancement of the CytoBuster™ Protein Extraction reagent, includes four phosphatase inhibitors. PhosphoSafe is compatible with Western blotting, immunoprecipitations, and kinase assays.
Purification of His•Tag fusion proteins by metal chelation chromatographyThe His•Bind® family of products offers a wide selection of supports designed for rapid one-step purification of proteins containing the His•Tag® sequence by immobilized metal affinity chromatography (IMAC). The His•Tag sequence (6, 8 or 10 consecutive histidine residues) binds to divalent cations (Ni2+) immobilized on NTA*- and IDA-based His•Bind resins.…
miVac is a range of centrifugal vacuum concentrators and freeze driers capable of removing water and organic solvents from a variety of sample formats including tubes, microplates, and vials. miVac concentrators come in 2 sizes, the smaller Duo and a larger Quattro. There is a choice of vacuum pump and rotor according to application and sample format. To speed concentration built-in special runs for working with water and…
RFP -Trap® - use your fluorescence fusion as tag for biochemical studies Curiously RFP, a widely used labeling tag in cell biology, is rarely used for biochemical analyses. This may be due in part to limited RFP antibody availability and specificity as well as co-eluted heavy and light antibody chains that interfere with subsequent analyses. Superior results in a fraction of the time The ChromoTek RFP-Trap® overcomes these pro…
Fusion of proteins to Glutathione S-Transferase (GST) of Schistosoma japonicum is a common technique to increase solubility and expression level of a protein. Furthermore, GST-tagged fusion proteins are often used in protein-protein interaction studies. Both approaches require highly specific tools to isolate and detect GST fusion proteins. GST-Trap The GST-Trap is a highly specific and robust GST-binding protein coupled to ma…
DNA methylation was the first epigenetic modification identified and has been intensively studied for more than half a century. By now it is clear that Dnmt1, the major eukaryotic DNA methyltransferase, faithfully maintains genome-wide methylation patterns and plays an essential role in the epigenetic network controlling gene expression and genome stability during development. However, the molecular mechanisms that ultimately…
Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles DNA and acts as a loading platform for multiple, transiently interacting factors participating in various DNA transactions. PCNA plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodeling. All these cellular processes are regulated by a complex n…
cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC (Immobilized Metal Affinity Chromatography) matrix for convenient single-step purification of His-tagged proteins from lysates. Roche's new proprietary nickel-chelate chemistry is compatible with commonly used reducing agents (such as DTT), chelating metalloprotease inhibitors (such as EDTA), and a wide range of buffer substances and salt conditions. The w…
Introducing an all-in-one centrifugal device for the protein purification workflow.Traditional protein purification is a long process with many steps and multiple devices, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon® Pro purification system. No matter what your workfl…
The PureEpi Chromatin Preparation and Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues. A detailed protocol is provided that includes examples of the many variables involved in making chromatin on multiple instruments and how they may affect ChIP results. Features & Benefits • Complete set of materials for up to…
