Western blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The Axygen Power Supplies are full-featured power sources ideal for molecular biology workflows including DNA and protein gel applications. The compact user interface has 4 sets of color-coded terminals allowing for use with up to 4 gel boxes simultaneously. Depending on the model, operating modes include constant voltage, current, or power all with automatic crossover feature. The PS250HA has the ability to set programs with…
The Axygen Power Supplies are full-featured power sources ideal for molecular biology workflows including DNA and protein gel applications. The compact user interface has 4 sets of color-coded terminals allowing for use with up to 4 gel boxes simultaneously. Depending on the model, operating modes include constant voltage, current, or power all with automatic crossover feature. The PS300GP can be quickly programmed to run with…
Biotin labelling of protein, amine reactive. Only 1 hour to get conjugates, all processes in a single filtration tube, high recovery of conjugates, applicable for 50-200 ug IgG
Our Chemifluorescence Western Blotting kit allows sensitive, Chemifluorescent detection of specific Protein targets. Attomolar detection sensitivity Non-isotopic detection with low background Quantitation over four orders of magnitude Optimized for use with the FMBIO® Fluorescence Imaging System During a chemifluorescent assay, a nonfluorogenic substrate is coverted to a fluorescent product by an enzyme such as alkaline phos…
Our Chemifluorescence Western Blotting kit allows sensitive, Chemifluorescent detection of specific Protein targets. Attomolar detection sensitivity Non-isotopic detection with low background Quantitation over four orders of magnitude Optimized for use with the FMBIO® Fluorescence Imaging System During a chemifluorescent assay, a nonfluorogenic substrate is coverted to a fluorescent product by an enzyme such as alkaline phos…
Dedicated electroblotting for mini and maxi gels Recently redesigned to accommodate up to 4 electroblotting cassettes, the Scie-Plas TV100-EBK and TV400-EBK electroblotters provide a cost-effective and safe and easy to use system for the transfer of proteins and nucleic acids from 10 x 10cm mini and 20.5 x 20cm maxi gels. Both units are supplied with asymmetric colour-coded cassettes as a unique design feature to prevent them…
These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting, typically 15 to 30 minutes. All units can be used for all types of blotting: western, southern and northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Each unit is compatible with their respective omniPAGE vertical gel system. Semi Dry…
These Capillary Blotting systems offer a contained unit for southern and northern blotting. These units incorporate optimized permanent wicks which eliminate the requirement for overlapping wicks, offering greater consistency. The porous polyethylene platform draws up transfer buffer faster than traditional systems allowing for faster transfer times. Side by side transfer of multiple gels can be performed in a single set up. A…
Four different sample number size and style of Hybridisation Manifold are offered: two types of dot blotter and two types of slot blotter.Typical applications include clone screening with DNA / RNA probes in Southern / Northern blots and immunological screening with antibodies in western blots. The units incorporate precision lapped surfaces to ensure uniform blotting membrane contact and a leak proof gasket. These prevent lat…
ApplicationThe kit is used for the quantitative determination of BrdU incorporated into cellular DNA using a 96-well microplate cell ELISA format. Benefits Safer: No radioisotopes are used. Accurate: The results strongly correlate to those obtained with the [3H]-thymidine method. Sensitive: As sensitive as [3H]-thymidine. Fast: The use of multiwell ELISA readers allows for processing of a large number of samples. Ea…
