High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
o Excellent retention and alternative selectivity for polar compoundso Completely stable under 100% aqueous conditionso Ideal for LC/MS applications Product detail: AQUASIL C18 columns provide more retention for difficult-to-retain polar compounds due to a unique hydrophillic end-capping. The combination of a moderate coverage C18 group and an additional polar group gives new selectivity compared to a traditional C18.…
HyPURITY ADVANCE Columns - offer exceptional chromatographic performance and is ideal for high throughoput laboratories with complex and variable sample types. Product detail: HyPURITY ADVANCE contains a polar amide group embedded within a C8 chain, and is fully endcapped. The columns are based on our metal free HyPURITY silica and, compared to other phases, produces a competely new selectivity. A polar functiona…
Hypersil BDS C18 Columnso Sharp peaks and improved symmetry for basic compoundso Value and performance from a globally referenced columno Long column lifetimes with excellent column-to-column and batch-to-batch reproduciblity Product detail:Hypersil BDS C18 receives a special base deactivation treatment during silica material manufacture to reduce silanophilic activity. Hypersil BDS C18 is endcapped and suitable for chro…
o Based on ultra pure 190 Å pore size silica for analysis of small molecules to proteinso Extensive quality control tests to ensure the ultimate in reproducibility and performanceo Unbeatable peak shapes for all sample typeso Select from five phases for a wide range of selectivity options Product detail: HyPURITY C18 columns are based on the latest in silica technology - HyPURITY silica, which has less than 10ppm total m…
To obtain precise information about the structure of synthetic peptides and proteins, it is imperative to achieve good separation (see illustration).Hypersil Amino acid columns have been developed for this purpose with excellent reproducibility and the highest reproducibility using high grade 5µm particle size silica with a high surface area. Achieve resolution of complex sequences of amino acids with sharp peak shapes.Hypersi…
High efficiency capillaries for high performance, sensitive separations 500, 320, 180, 100 & 75µm ID capillaries Availabe in a wide range of Thermo phases Rugged sleeved design for robustness 180µm ID flexible KAPPA for FinniganTM ProteomeX™ Workstation Product detail: Capillary and nanobore columns are ideal for applications which require high sensitivity. However, in order to take full benefit of the improvement i…
Run assays up to 5 times faster than a conventional HPLC. Increase mass spec sample throughput by a factor of 5. Eliminate dead time in your analysis and cut solvent usage by 99 percent. Achieve all this and more with the ExpressLC-100 system. TheExpressLC-100 system is a single-channel HPLC with binary solvent delivery, high pressure gradient mixing, variable volume injection, UV absorbance detection, and PC-based instrument…
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we h…
LabSolutions is Shimadzu´s software series for chromatography (GC, GCMS, LC, LCMS). The unified graphical interface makes it easy to work with Shimadzu chromatography products. The GCsolution and GCMSsolution is the perfect partner for the Shimadzu GC and GCMS product range. The GCsolution controls the GC-2010 and GC-2014 (from Ver. 2.3) as well as the previous models GC-14 and GC-17. The GCMSsolution Ver. 2 controls the GCMS-…
Agilent ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phase choices for method development optimization. These columns provide good peak shape over a wide pH range (2–9) for additional method development flexibility with one family of columns. Eclipse XDB columns can be used for method development at low pH (2–3) and the same column can be used for method development in the mid pH (6–8) region.
